Comparison of two fluorescence immunoassay methods for the detection of endocrine disrupting chemicals in water.

We describe two fluorescence immunoassays capable of detecting endocrine disrupting compounds in waste water. The first fluorescence method is a heterogeneous assay using total internal reflection fluorescence (TIRF) detection. The second method is a homogeneous assay that utilizes energy transfer (ETIA). Both fluorescence immunoassays are compared with respect to detection principle and ability to quantify the model analytes estrone, estradiol, and ethinylestradiol in a complex matrix regarding recovery rates and limits of detection. Calibrations were performed for the three analytes using both fluorescence methods. Limits of detection between 0.01 and 0.85 microg/l are achieved. In addition, measurements in synthetic waste water spiked with the analytes were performed. Both immunoassays allow the detection in waste water with recovery rates in the range of 70-112%.

[1]  Rosalind,et al.  Research needs for the risk assessment of health and environmental effects of endocrine disruptors: a report of the U.S. EPA-sponsored workshop. , 1996, Environmental health perspectives.

[2]  Lloyd A. Currie,et al.  Nomenclature in evaluation of analytical methods including detection and quantification capabilities1: (IUPAC Recommendations 1995) , 1999 .

[3]  G. Gauglitz,et al.  Surface modification for direct immunoprobes. , 1996, Biosensors & bioelectronics.

[4]  H. Komen,et al.  Feminization of male carp , 1996, Nature.

[5]  M. Pettersson,et al.  ETHINYLOESTRADIOL - AN UNDESIRED FISH CONTRACEPTIVE , 1999 .

[6]  H. Leffers,et al.  Comparison of short-term estrogenicity tests for identification of hormone-disrupting chemicals. , 1999 .

[7]  G. Gauglitz,et al.  New donor-acceptor pair for fluorescent immunoassays by energy transfer. , 1999, Bioconjugate chemistry.

[8]  T. Kubiak,et al.  Great Lakes embryo mortality, edema, and deformities syndrome (GLEMEDS) in colonial fish-eating birds: similarity to chick-edema disease. , 1991, Journal of toxicology and environmental health.

[9]  A R Woodward,et al.  Developmental abnormalities of the gonad and abnormal sex hormone concentrations in juvenile alligators from contaminated and control lakes in Florida. , 1994, Environmental health perspectives.

[10]  R. Holt,et al.  Pesticide and polychlorinated biphenyl residues in human adipose tissue from Northeast Louisiana , 1986, Bulletin of environmental contamination and toxicology.

[11]  D. J. Finney,et al.  Guidelines for immunoassay data processing. , 1985, Clinical chemistry.

[12]  G Gauglitz,et al.  Assessment of affinity constants by rapid solid phase detection of equilibrium binding in a flow system. , 1997, Journal of immunological methods.

[13]  Andreas Brecht,et al.  An integrated system for optical biomolecular interaction analysis , 1997 .