2A2-O05 Tracking of Ascidian Spermatozoa using High-speed Visual Feedback

We propose a novel automatic tracking system capable of tracking fast-moving, small objects under extreme conditions by using high-speed visual feedback for assessing sperm chemotaxis. The system shows remarkable performance and versatility, good enough for practical use in biology. Experimental results showed that we successfully achieved continuous stable tracking of swimming ascidian spermatozoa with quality sufficient for assessing sperm motility, indicating the feasibility of our system to tracking almost any type of cell.

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