Protein annotation by EBIMed

VOLUME 24 NUMBER 8 AUGUST 2006 NATURE BIOTECHNOLOGY is described, the control of cell lysis and passive leakage of proteins is highly relevant. The single presented lysis control (antiMalE) is not convincing as such and several cytoplasmic and periplasmic markers for cell lysis should be included. We agree completely with the conclusion made by Gál et al. that the controversial and apparently complex nature of the flagellar secretion signal should be investigated further. 1. Ghosh, P. Microbiol. Mol. Biol. Rev. 68, 771–795 (2004). 2. Végh, B. et al. Biochem. Biophys. Res. Commun. 345, 93–98 (2006). 3. Minamino, T. & Namba, K. J. Mol. Microbiol. Biotechnol. 7, 5–17 (2004) 4. Young, G.M. et al. Proc. Natl. Acad. Sci. USA 96, 6456–6461 (1999). 5. Lee, S. H. & Galán, J. Mol. Microbiol. 51, 483–495 (2004). 6. Ren, C– P. et al. J. Bacteriol. 186, 3547–3560 (2004). 7. Ren C.–P. et al. J. Bacteriol. 187, 1430–1440 (2005).