A linkage map of the porcine genome from a large-scale White Duroc x Erhualian resource population and evaluation of factors affecting recombination rates.

A porcine genome linkage map composed of 194 microsatellite markers was constructed with a large-scale White Duroc x Erhualian resource population. The marker order on this linkage map was consistent with the USDA-MARC reference map except for two markers on SSC3, two markers on SSC13 and two markers on SSCX. The length of the sex-averaged map (2344.9 cM) was nearly the same as that of the USDA-MARC and NIAI map. Highly significant heterogeneity in recombination rates between sexes was observed. Except for SSC1 and SSC13, the female autosomes had higher average recombination rates than the male autosomes. Moreover, recombination rates in the pseudoautosomal region were greater in males than in females. These observations are consistent with those of previous reports. The recombination rates on each paternal and maternal chromosome of F(2) animals were calculated. Recombination rates were not significantly affected by the age (in days) or parity of the F(1) animals. However, recombination rates on paternal chromosomes were affected by the mating season of the F(1) animals. This could represent an effect of environmental temperature on spermatogenesis.

[1]  L. Andersson,et al.  A comprehensive linkage map of the pig based on a wild pig-Large White intercross. , 2009, Animal genetics.

[2]  L. Alexander,et al.  Map assignments of 373 previously unreported porcine microsatellites. , 2005, Animal genetics.

[3]  D. Milan,et al.  A physical map of large segments of pig Chromosome 7q11–q14: comparative analysis with human Chromosome 6p21 , 2004, Mammalian Genome.

[4]  L. Alexander,et al.  Radiation hybrid mapping of 273 previously unreported porcine microsatellites. , 2002, Animal genetics.

[5]  L. Alexander,et al.  Radiation hybrid mapping of 118 new porcine microsatellites. , 2002, Animal genetics.

[6]  E. Kobayashi,et al.  A linkage map of 243 DNA markers in an intercross of Göttingen miniature and Meishan pigs. , 1999, Animal genetics.

[7]  M. Neff,et al.  A second-generation genetic linkage map of the domestic dog, Canis familiaris. , 1999, Genetics.

[8]  M. Groenen,et al.  A comprehensive microsatellite linkage map of the chicken genome. , 1998, Genomics.

[9]  D. I. Våge,et al.  A medium-density genetic linkage map of the bovine genome , 1997, Mammalian Genome.

[10]  K Lange,et al.  Descent graphs in pedigree analysis: applications to haplotyping, location scores, and marker-sharing statistics. , 1996, American journal of human genetics.

[11]  L. Alexander,et al.  A comprehensive map of the porcine genome. , 1996, Genome research.

[12]  A. Rose,et al.  Mutant rec-1 eliminates the meiotic pattern of crossing over in Caenorhabditis elegans. , 1995, Genetics.

[13]  Muladno,et al.  The PiGMaP consortium linkage map of the pig (Sus scrofa) , 1995, Mammalian Genome.

[14]  L. Andersson,et al.  A primary linkage map of the porcine genome reveals a low rate of genetic recombination. , 1994, Genetics.

[15]  P. Green,et al.  Guidelines for human linkage maps: an international system for human linkage maps (ISLM, 1990). , 1991, Genomics.

[16]  M. Daly,et al.  A genetic linkage map of the human genome , 1987, Cell.

[17]  P. Perry,et al.  Recombination in male and female meiocytes contrasted. , 1977, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[18]  K. Lange,et al.  Age trends in human chiasma frequencies and recombination fractions. I. Chiasma frequencies. , 1975, American journal of human genetics.

[19]  G. Simchen,et al.  Fine and Coarse Controls of Genetic Recombination , 1969, Nature.

[20]  L. C. Dunn,et al.  Sex differences in recombination of linked genes in animals. , 1967, Genetical research.

[21]  J. Renwick,et al.  Male and female recombination fractions for the nail‐patella: ABO linkage in man , 1965 .

[22]  C. Bridges.  A linkage variation in Drosophila , 1915 .

[23]  J. Ren,et al.  Growth and meat quality QTL in pigs with special reference to a very large white Duroc × Erhualian resource population. , 2006 .

[24]  J C Murray,et al.  Pediatrics and , 1998 .

[25]  L. Alexander,et al.  A microsatellite linkage map of the porcine genome. , 1994, Genetics.

[26]  K. Sandberg,et al.  Genetic linkage in the horse. II. Distribution of male recombination estimates and the influence of age, breed and sex on recombination frequency. , 1984, Genetics.

[27]  R C Elston,et al.  Age trends in human chiasma frequencies and recombination fractions. II. Method for analyzing recombination fractions and applications to the ABO:nail-patella linkage. , 1976, American journal of human genetics.

[28]  E. Thorsby,et al.  The relation of parental sex and age to recombination in the HL-A system. , 1973, Human heredity.