Matching Gene Expression with Hypometabolism after Cerebral Ischemia in the Nonhuman Primate

To correlate brain metabolic status with the molecular events during cerebral ischemia, a cDNA array was performed after positron emission tomography scanning in a model of focal cerebral ischemia in baboons. Cluster analysis for the expression of 74 genes allowed the identification of 4 groups of genes. In each of the distinct groups, the authors observed a marked inflection in the pattern of gene expression when the CMRo2 was reduced by 48% to 66%. These patterns of coordinated modifications in gene expression could define molecular checkpoints for the development of an ischemic infarct and a molecular definition of the penumbra.

[1]  F. Sharp,et al.  Multiple Molecular Penumbras after Focal Cerebral Ischemia , 2000, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[2]  Otto W Witte,et al.  Gene Expression Profiling in Perilesional and Contralateral Areas after Ischemia in Rat Brain , 2002, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[3]  V Beaudouin,et al.  Sequential studies of severely hypometabolic tissue volumes after permanent middle cerebral artery occlusion. A positron emission tomographic investigation in anesthetized baboons. , 1995, Stroke.

[4]  G. Mies,et al.  Evolution of Brain Infarction after Transient Focal Cerebral Ischemia in Mice , 2000, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[5]  A. Dehejia,et al.  The genexpress IMAGE knowledge base of the human muscle transcriptome: a resource of structural, functional, and positional candidate genes for muscle physiology and pathologies. , 1999, Genome research.

[6]  K. Jin,et al.  Microarray analysis of hippocampal gene expression in global cerebral ischemia , 2001, Annals of neurology.

[7]  S. Bates,et al.  Stroke Genomics: Approaches to Identify, Validate, and Understand Ischemic Stroke Gene Expression , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[8]  C. Auffray,et al.  Reverse transcription in the presence of dideoxynucleotides to increase the sensitivity of expression monitoring with cDNA arrays. , 1999, BioTechniques.

[9]  Z. Ke.  Mapping the ischemic penumbra with PET , 2000 .

[10]  J. Baron,et al.  Relationships between High Oxygen Extraction Fraction in the Acute Stage and Final Infarction in Reversible Middle Cerebral Artery Occlusion: An Investigation in Anesthetized Baboons with Positron Emission Tomography , 1996, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[11]  C. Auffray,et al.  The Genexpress IMAGE knowledge base of the human brain transcriptome: a prototype integrated resource for functional and computational genomics. , 1999, Genome research.

[12]  U. Certa,et al.  Parallel Gene Expression Monitoring using Oligonucleotide Probe Arrays of Multiple Transcripts with an Animal Model of Focal Ischemia , 2000, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[13]  E. Mackenzie,et al.  The ischaemic penumbra , 2001, Current opinion in neurology.

[14]  E. Mackenzie,et al.  Increased Expression of Transforming Growth Factor-β after Cerebral Ischemia in the Baboon: An Endogenous Marker of Neuronal Stress? , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[15]  P. Lipton,et al.  Ischemic cell death in brain neurons. , 1999, Physiological reviews.