Antibody–nucleotide conjugate as a substrate for DNA polymerases† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01839a

Antibody-modified nucleotides are developed and their incorporation into nascent DNA by DNA polymerases exploited for the development of the naked-eye detection of DNA.

[1]  Rigid, conjugated, fluoresceinated thymidine triphosphates: syntheses and polymerase mediated incorporation into DNA analogues. , 2003, Chemistry.

[2]  Nancy F. Hansen,et al.  Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry , 2008, Nature.

[3]  A. Armston.  The immunoassay handbook , 2014, Annals of clinical biochemistry.

[4]  B. Bilgicer,et al.  Conjugation of a reactive thiol at the nucleotide binding site for site-specific antibody functionalization. , 2014, Bioconjugate chemistry.

[5]  Robert B. Hartlage,et al.  This PDF file includes: Materials and Methods , 2009 .

[6]  Xiaohui Wang,et al.  Simple Method To Prepare Oligonucleotide-Conjugated Antibodies and Its Application in Multiplex Protein Detection in Single Cells. , 2016, Bioconjugate chemistry.

[7]  E. Engvall,et al.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. , 1971, Immunochemistry.

[8]  F. Rutjes,et al.  Strain-Promoted 1,3-Dipolar Cycloaddition of Cycloalkynes and Organic Azides , 2016, Topics in Current Chemistry.

[9]  A. Marx,et al.  Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides. , 2016, Bioorganic & medicinal chemistry letters.

[10]  S. Quake,et al.  Effects of a Modified Dye-Labeled Nucleotide Spacer Arm on Incorporation by Thermophilic DNA Polymerases , 2006, Nucleosides, Nucleotides & Nucleic Acids.

[11]  D C Ward,et al.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[12]  A. Marx,et al.  Structural Insights into the Processing of Nucleobase-Modified Nucleotides by DNA Polymerases. , 2016, Accounts of chemical research.

[13]  Y. Ishino,et al.  DNA polymerases as useful reagents for biotechnology – the history of developmental research in the field , 2014, Front. Microbiol..

[14]  C. Anderson,et al.  Comparison of Conjugation Strategies of Cross-Bridged Macrocyclic Chelators with Cetuximab for Copper-64 Radiolabeling and PET Imaging of EGFR in Colorectal Tumor-Bearing Mice , 2014, Molecular pharmaceutics.

[15]  T. Waritani,et al.  An ELISA protocol to improve the accuracy and reliability of serological antibody assays , 2017, MethodsX.

[16]  A. Marx,et al.  Sequence-Specific Incorporation of Enzyme-Nucleotide Chimera by DNA Polymerases. , 2016, Angewandte Chemie.

[17]  A. Waggoner,et al.  Directly labeled DNA probes using fluorescent nucleotides with different length linkers. , 1994, Nucleic acids research.

[18]  S. Quake,et al.  Single-Molecule DNA Sequencing of a Viral Genome , 2008, Science.

[19]  Suleyman Aydin,et al.  A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA , 2015, Peptides.

[20]  Michal Hocek.  Synthesis of base-modified 2'-deoxyribonucleoside triphosphates and their use in enzymatic synthesis of modified DNA for applications in bioanalysis and chemical biology. , 2014, The Journal of organic chemistry.

[21]  W. Ganzenmüller.  Ein italienisches Goldlüsterrezept vom Ende des 14. Jahrhunderts , 1938 .

[22]  Gonçalo J. L. Bernardes,et al.  Construction of homogeneous antibody–drug conjugates using site-selective protein chemistry , 2016, Chemical science.

[23]  Sung Jae Kim,et al.  Increasing the sensitivity of enzyme-linked immunosorbent assay using multiplexed electrokinetic concentrator. , 2010, Analytical chemistry.

[24]  A. Nehorai,et al.  Antibody quantum dot conjugates developed via copper-free click chemistry for rapid analysis of biological samples using a microfluidic microsphere array system. , 2014, Bioconjugate chemistry.

[25]  P. Ross,et al.  Physical and Chemical Stability of Antibody Drug Conjugates: Current Status. , 2016, Journal of pharmaceutical sciences.

[26]  J. Otlewski,et al.  Current methods for the synthesis of homogeneous antibody-drug conjugates. , 2015, Biotechnology advances.

[27]  A. Marx,et al.  DNA polymerase-catalyzed incorporation of nucleotides modified with a G-quadruplex-derived DNAzyme. , 2015, Chemical communications.