CBF/CMRO2 may be quantified using PET with 15O-tracers, but the conventional three-step technique (3S) requires a long study period, attributed to need for separate acquisition for three radioactive tracers for CBF (H215O or C15O2), CMRO2 (15O2), and CBV (C15O). Simultaneous determination of CBF, CMRO2 and CBV from a single dynamic 15O2 scan is known to suffer from statistical uncertainty. We recently developed a rapid technique that allows sequential administration of two of three tracers (for CBF and CMRO2) in a single PET scan, and generates quantitative CBF/CMRO2 images with a dual-tracer integration method (DTI). This study asked whether this technique is feasible in normal human subjects and how accurate quantitative values it provides in comparison with the 3S and non-linear fitting method (NLM). Eight normal subjects (24.2+-2.6 y.o) participated in this study. The protocol of the study was approved by the local ethical committee. All subjects received three emission scans, i.e., a 4-min scan after short bolus administration of C15O, a 9.5-min scan with dual administration of C15O2 followed by 15O2, and a 9-min scan with 15O2 followed by H215O. With correction for blood volume using the first C15O scan data, CBF/CMRO2 images were generated by 3S using the first part of the second (CO2) and the third scan (O2), whereas those by DTI method were calculated using both part of PET data in each of the second or the third scan. The calculation used a mathematical formula derived from a single-tissue compartment model including a correction for blood volume and an assumed partition coefficient for water. Regional quantitative values of CBF/CMRO2 in the DTI was compared with those by 3S and NLM. We also compared by voxel-by-voxel CBF or CMRO2 images between the second and the third DTI scan to test the regional difference. Global CBF or CMRO2 values for the DTI did not differ from those by the 3S, and between the second and third DTI scans. The cortical regional values of the DTI neither differed significantly from those of 3S and NLM. In contrast, CMRO2 values of the white matter region in both 3S and DTI differed significantly from NLM, as was predicted from a simulation study for the error in the assumed partition coefficient. Voxel-based comparison of CBF images between the second and the third DTI scan showed significant difference at brain regions around nasal cavity, and at the somatosensory areas that may come from the difference in methodology (inhalation or venous-injection of CBF tracer) and physiology (sensation of intravenous injection), respectively. These were also seen when compared CMRO2 images, but at a lower height threshold. These results suggest that this DTI technique generates CBF/CMRO2 images with a considerably shortened scan period, which are comparable to those by 3S, in terms of quantitative accuracy and image quality. The technique may be of use for clinical studies in patients particularly with acute stroke, and also for investigation of metabolic coupling during neuronal activation or pharmacological challenges in human.