Fabricating of Acetylcholine Biosensor by a Layer‐by‐layer Deposition Technique for Determining Trichlorfon

An acetylcholine (ACh) biosensor has been fabricated with bienzymes/poly(diallyldimethylammonium chloride) (PDDA) multilayer film-modified platinum (Pt) electrodes by a layer-by-layer technique (LBL). The ACh biosensor was optimized and the properties are described. This ACh biosensor was used for the detection of organophosphate pesticide trichlorfon. The detection limits (found 0.001 μg/mL for trichlorfon) make it possible to detect the pollutants. This simple protocol of biosensor preparation, high sensitivity and stability are very promising for the determination of environmental pollutants in field conditions.

[1]  M. Šnejdárková,et al.  Acetylcholine minisensor based on metal‐supported lipid bilayers for determination of environmental pollutants , 1997 .

[2]  Qiang Chen,et al.  Avidin-Biotin System-Based Enzyme Multilayer Membranes for Biosensor Applications: Optimization of Loading of Choline Esterase and Choline Oxidase in the Bienzyme Membrane for Acetylcholine Biosensors , 1998 .

[3]  J. Anzai,et al.  Selective permeation of hydrogen peroxide through polyelectrolyte multilayer films and its use for amperometric biosensors. , 2001, Analytical chemistry.

[4]  Dimitrios P. Nikolelis,et al.  Acetylcholine Biosensor Based on Dendrimer Layers for Pesticides Detection , 2003 .

[5]  G. Evtugyn,et al.  Polyaniline-modified cholinesterase sensor for pesticide determination. , 2002, Bioelectrochemistry.

[6]  P. Atanasov,et al.  Development of a Disposable Organophosphate Biosensor , 1999 .

[7]  F. Díaz-Fierros,et al.  Measurement of cholinesterase activity inhibition for the detection of organophosphorus and carbamate pesticides in water , 1995 .

[8]  Vasif Hasirci,et al.  Determination of binary pesticide mixtures by an acetylcholinesterase-choline oxidase biosensor. , 2004, Biosensors & bioelectronics.

[9]  A Mulchandani,et al.  Amperometric microbial biosensor for direct determination of organophosphate pesticides using recombinant microorganism with surface expressed organophosphorus hydrolase. , 2001, Biosensors & bioelectronics.

[10]  Hidemoto Nakagawa,et al.  Re-activation of an amperometric organophosphate pesticide biosensor by 2-pyridinealdoxime methochloride , 2000 .

[11]  A. Ciucu,et al.  Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system. , 2003, Biosensors & bioelectronics.

[12]  A. Michael,et al.  Amperometric sensors for peroxide, choline, and acetylcholine based on electron transfer between horseradish peroxidase and a redox polymer. , 1993, Analytical chemistry.

[13]  Doretti,et al.  Acetylcholine biosensor involving entrapment of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane. , 2000, Enzyme and microbial technology.

[14]  Jean-Louis Marty,et al.  Screen-printed electrode based on AChE for the detection of pesticides in presence of organic solvents. , 2002, Talanta.

[15]  I. Karube,et al.  Development of acetylcholine sensor using carbon fiber (amperometric determination). , 1991, Biosensors & bioelectronics.

[16]  J. Anzai,et al.  Controlled deposition of glucose oxidase on platinum electrode based on an avidin/biotin system for the regulation of output current of glucose sensors. , 1995, Analytical chemistry.

[17]  T. H. Milby,et al.  Worker poisonings due to paraoxon residues. , 1977, Journal of occupational medicine. : official publication of the Industrial Medical Association.

[18]  Andrea Cagnini,et al.  DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING , 1995 .