Development of fermented Hericium erinaceus juice with high content of L-glutamine and L-glutamic acid

Summary Fermented plant beverages (FPB) with a high content of desirable principle components are served as functional foods from several years. Hericium erinaceus is famous for its antimicrobial, antioxidant, antihypertensive and antidiabetic nature. Accordingly, the current study was aimed to produce fermented H. erinaceus juice with a high content of L-glutamine (Gln) and L-glutamic acid (GA) through lactic acid bacteria (LAB) isolated from fermented Thai foods. LAB isolates were screened and identified the potent protease-producing bacteria Enterococcus faecalis (G414/1) that facilitate the production of Gln and GA through protein hydrolysis. Box–Behnken design (BBD) and response surface methodology (RSM) were adapted for the optimisation of conditions for the increased production of Gln and GA during fermentation of H. erinaceus. We succeeded with an optimum concentration of cofactor (CaCl2), pH and temperature for improved protease activity and subsequent Gln and GA production. The ability of isolated E. faecalis strain to produce Gln and GA was demonstrated in this study. Further, upstream processes like strain improvement and media optimisation will direct the way to produce enriched H. erinaceus based FPB.

[1]  B. Sivamaruthi,et al.  Evaluation of factors that influence the L-glutamic and γ-aminobutyric acid production during Hericium erinaceus fermentation by lactic acid bacteria , 2016 .

[2]  L. Jespersen,et al.  The Use of Lactic Acid Bacteria Starter Culture in the Production of Nunu, a Spontaneously Fermented Milk Product in Ghana , 2014, International journal of food science.

[3]  B. Sivamaruthi,et al.  Screening and kinetics of glutaminase and glutamate decarboxylase producing lactic acid bacteria from fermented Thai foods , 2014 .

[4]  D. Kantachote,et al.  Bioaugmentation of latex rubber sheet wastewater treatment with stimulated indigenous purple nonsulfur bacteria by fermented pineapple extract , 2014 .

[5]  Sung-Hee Park,et al.  STATISTICAL OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION FROM BRACKISH ENVIRONMENT Bacillus sp. SKK11 BY SSF USING HORSE GRAM HUSK , 2014, Preparative biochemistry & biotechnology.

[6]  N. Saari,et al.  Modeling of glutamic acid production by Lactobacillus plantarum MNZ , 2013 .

[7]  C. L. L. Ferreira,et al.  The genus Enterococcus as probiotic: safety concerns , 2013 .

[8]  P. Wilson,et al.  Production and characterisation of protease enzyme produced by a novel moderate thermophilic bacterium (EP1001) isolated from an alkaline hot spring, Zimbabwe , 2012 .

[9]  L. Jabalameli,et al.  Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park , 2011, Enzyme research.

[10]  D. Kantachote,et al.  Enhancement of γ-aminobutyric acid in a fermented red seaweed beverage by starter culture Lactobacillus plantarum DW12 , 2011 .

[11]  Egwim Ec,et al.  Proximate composition, phytochemical screening and antioxidant activity of ten selected wild edible Nigerian mushrooms , 2011 .

[12]  Miguel Anxo Murado,et al.  Recovery of Proteolytic and Collagenolytic Activities from Viscera By-products of Rayfish (Raja clavata) , 2009, Marine drugs.

[13]  F. Herold,et al.  Biosynthesis of selenium-containing polysaccharides with antioxidant activity in liquid culture of Hericium erinaceum , 2009 .

[14]  M. Garro,et al.  Enzymatic hydrolysis of soybean protein using lactic acid bacteria , 2008 .

[15]  Sunil Kumar,et al.  Application of Box-Benhken experimental design for optimization of laccase production by Coriolus versicolor MTCC138 in solid-state fermentation , 2008 .

[16]  Jun Huang,et al.  Biosynthesis of γ-aminobutyric acid (GABA) using immobilized whole cells of Lactobacillus brevis , 2007 .

[17]  S. A. Rasool,et al.  Screening, Isolation and Characterization of Alkaline Protease Producing Bacteria from Soil , 2006 .

[18]  H. Ingmer,et al.  Proteolytic systems of lactic acid bacteria , 2006, Applied Microbiology and Biotechnology.

[19]  L De Vuyst,et al.  The role and application of enterococci in food and health. , 2006, International journal of food microbiology.

[20]  S. Bakalova,et al.  Evidence for Proteolytic Activity of Lactobacilli Isolated from Kefir Grains , 2006 .

[21]  J. Swings,et al.  Phenotypic and genetic diversity of enterococci isolated from Italian cheeses , 2001, Journal of Dairy Research.

[22]  D. Juang,et al.  The applicability of the API 20E and API Rapid NFT systems for the identification of bacteria from activated sludge , 2001 .

[23]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[24]  Seung-Ryeul Shin,et al.  Characteristics of Hericium erinaceus and its Extracts , 2003 .

[25]  M. Rea,et al.  Biochemical properties of enterococci relevant to their technological performance , 2001 .

[26]  A. Weis,et al.  Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: a modern perspective. , 1999, Critical reviews in immunology.

[27]  A. Haandrikman,et al.  Proteolytic enzymes of lactic acid bacteria , 1997 .