Reversible thermo-pneumatic valves on centrifugal microfluidic platforms.

Centrifugal microfluidic systems utilize a conventional spindle motor to automate parallel biochemical assays on a single microfluidic disk. The integration of complex, sequential microfluidic procedures on these platforms relies on robust valving techniques that allow for the precise control and manipulation of fluid flow. The ability of valves to consistently return to their former conditions after each actuation plays a significant role in the real-time manipulation of fluidic operations. In this paper, we introduce an active valving technique that operates based on the deflection of a latex film with the potential for real-time flow manipulation in a wide range of operational spinning speeds. The reversible thermo-pneumatic valve (RTPV) seals or reopens an inlet when a trapped air volume is heated or cooled, respectively. The RTPV is a gas-impermeable valve composed of an air chamber enclosed by a latex membrane and a specially designed liquid transition chamber that enables the efficient usage of the applied thermal energy. Inputting thermo-pneumatic (TP) energy into the air chamber deflects the membrane into the liquid transition chamber against an inlet, sealing it and thus preventing fluid flow. From this point, a centrifugal pressure higher than the induced TP pressure in the air chamber reopens the fluid pathway. The behaviour of this newly introduced reversible valving system on a microfluidic disk is studied experimentally and theoretically over a range of rotational frequencies from 700 RPM to 2500 RPM. Furthermore, adding a physical component (e.g., a hemispherical rubber element) to induce initial flow resistance shifts the operational range of rotational frequencies of the RTPV to more than 6000 RPM. An analytical solution for the cooling of a heated RTPV on a spinning disk is also presented, which highlights the need for the future development of time-programmable RTPVs. Moreover, the reversibility and gas impermeability of the RTPV in the microfluidic networks are validated on a microfluidic disk designed for performing liquid circulation. Finally, an array of RTPVs is integrated into a microfluidic cartridge to enable sequential aliquoting for the conversion of dengue virus RNA to cDNA and the preparation of PCR reaction mixtures.

[1]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[2]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[3]  R. Mathies,et al.  Monolithic integrated microfluidic DNA amplification and capillary electrophoresis analysis system , 2000 .

[4]  Igor L. Medintz,et al.  Single-molecule DNA amplification and analysis in an integrated microfluidic device. , 2001, Analytical chemistry.

[5]  R. Mathies,et al.  Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis. , 2001, Lab on a chip.

[6]  Guangshan Li,et al.  Microfluidic devices for fluidic circulation and mixing improve hybridization signal intensity on DNA arrays. , 2003, Lab on a chip.

[7]  Vincent Studer,et al.  Scaling properties of a low-actuation pressure microfluidic valve , 2004 .

[8]  I. V. Shevchuk,et al.  Transient laminar conjugate heat transfer of a rotating disk: theory and numerical simulations , 2004 .

[9]  Paul Yager,et al.  Recirculating flow accelerates DNA microarray hybridization in a microfluidic device. , 2006, Lab on a chip.

[10]  I. V. Shevchuk.  Unsteady conjugate laminar heat transfer of a rotating non-uniformly heated disk: Application to the transient experimental technique , 2006 .

[11]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[12]  Chong H. Ahn,et al.  Institute of Physics Publishing Journal of Micromechanics and Microengineering a Review of Microvalves , 2022 .

[13]  Yoon‐Kyoung Cho,et al.  Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices. , 2007, Lab on a chip.

[14]  D. Xing,et al.  Micropumps, microvalves, and micromixers within PCR microfluidic chips: Advances and trends. , 2007, Biotechnology advances.

[15]  Igor V. Shevchuk,et al.  Convective Heat and Mass Transfer in Rotating Disk Systems , 2009 .

[16]  Jintae Kim,et al.  Centrifugal microfluidics for biomedical applications. , 2010, Lab on a chip.

[17]  Roland Zengerle,et al.  Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). , 2010, Lab on a chip.

[18]  Roland Zengerle,et al.  Microstructuring of polymer films for sensitive genotyping by real-time PCR on a centrifugal microfluidic platform. , 2010, Lab on a chip.

[19]  Dermot Diamond,et al.  Optically addressable single-use microfluidic valves by laser printer lithography. , 2010, Lab on a chip.

[20]  Jens Ducrée,et al.  Liquid recirculation in microfluidic channels by the interplay of capillary and centrifugal forces , 2010 .

[21]  Adisorn Tuantranont,et al.  DNA hybridization enhancement using piezoelectric microagitation through a liquid coupling medium. , 2011, Lab on a chip.

[22]  Yoon-Kyoung Cho,et al.  Elastomeric membrane valves in a disc. , 2011, Lab on a chip.

[23]  Rustem F Ismagilov,et al.  Digital isothermal quantification of nucleic acids via simultaneous chemical initiation of recombinase polymerase amplification reactions on SlipChip. , 2011, Analytical chemistry.

[24]  Albert Folch,et al.  Microvalves and Micropumps for BioMEMS , 2011, Micromachines.

[25]  W. Marsden.  I and J , 2012 .

[26]  L. Yerushalmi,et al.  Mixing characteristics and liquid circulation in a new multi-environment bioreactor , 2013, Bioprocess and Biosystems Engineering.

[27]  Mary Amasia,et al.  Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving , 2012 .

[28]  Jens Ducrée,et al.  Centrifugo-pneumatic valving utilizing dissolvable films. , 2012, Lab on a chip.

[29]  Jens Ducrée,et al.  At-line bioprocess monitoring by immunoassay with rotationally controlled serial siphoning and integrated supercritical angle fluorescence optics. , 2013, Analytica chimica acta.

[30]  R. Burger,et al.  Comprehensive integration of homogeneous bioassays via centrifugo-pneumatic cascading. , 2013, Lab on a chip.

[31]  Fatimah Ibrahim,et al.  Vacuum/Compression Valving (VCV) Using Parrafin-Wax on a Centrifugal Microfluidic CD Platform , 2013, PloS one.

[32]  Jens Ducrée,et al.  Fluidic Automation of Nitrate and Nitrite Bioassays in Whole Blood by Dissolvable-Film Based Centrifugo-Pneumatic Actuation , 2013, Sensors.

[33]  Fatimah Ibrahim,et al.  Push pull microfluidics on a multi-level 3D CD. , 2013, Lab on a chip.

[34]  Roland Zengerle,et al.  Rapid and highly sensitive luciferase reporter assay for the automated detection of botulinum toxin in the centrifugal microfluidic LabDisk platform , 2013 .

[35]  Jintae Kim,et al.  Electrochemical velocimetry on centrifugal microfluidic platforms. , 2013, Lab on a chip.

[36]  Tae-Hyeong Kim,et al.  Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms. , 2013, Lab on a chip.

[37]  Minseok S Kim,et al.  Fully automated circulating tumor cell isolation platform with large-volume capacity based on lab-on-a-disc. , 2014, Analytical chemistry.

[38]  Lee A. Walsh,et al.  Spin coating of hydrophilic polymeric films for enhanced centrifugal flow control by serial siphoning , 2014 .

[39]  Robert Burger,et al.  Automation of immunoassays through centrifugal lab-on-a-disc platforms , 2014 .

[40]  Dermot Diamond,et al.  A portable centrifugal analyser for liver function screening. , 2014, Biosensors & bioelectronics.

[41]  Fatimah Ibrahim,et al.  Latex micro-balloon pumping in centrifugal microfluidic platforms. , 2014, Lab on a chip.

[42]  Philip N Duncan,et al.  Microfluidic serial dilution ladder. , 2014, The Analyst.

[43]  Jens Ducrée,et al.  Event-triggered logical flow control for comprehensive process integration of multi-step assays on centrifugal microfluidic platforms. , 2014, Lab on a chip.

[44]  Tae-Hyeong Kim,et al.  Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. , 2014, Analytical chemistry.

[45]  M. Madou,et al.  Gating valve on spinning microfluidic platforms: A flow switch/control concept , 2014 .

[46]  Roland Zengerle,et al.  Real-time PCR based detection of a panel of food-borne pathogens on a centrifugal microfluidic “LabDisk” with on-disk quality controls and standards for quantification , 2014 .

[47]  Jens Ducrée,et al.  Solvent-selective routing for centrifugally automated solid-phase purification of RNA , 2014, Microfluidics and Nanofluidics.

[48]  M. Bergeron,et al.  From cellular lysis to microarray detection, an integrated thermoplastic elastomer (TPE) point of care Lab on a Disc. , 2015, Lab on a chip.

[49]  Jiwen Xiang,et al.  A magnetically actuated valve for centrifugal microfluidic applications , 2015 .

[50]  Fatimah Ibrahim,et al.  Guided routing on spinning microfluidic platforms , 2015 .

[51]  Hamzah Arof,et al.  Development of novel passive check valves for the microfluidic CD platform , 2015 .

[52]  Tae Seok Seo,et al.  Integrated centrifugal reverse transcriptase loop-mediated isothermal amplification microdevice for influenza A virus detection , 2014, Biosensors and Bioelectronics.

[53]  F. Schwemmer,et al.  Centrifugal microfluidic platforms: advanced unit operations and applications. , 2015, Chemical Society reviews.

[54]  Erin J Templeton,et al.  Rapid prototyping of pneumatically actuated hydrocarbon gel valves for centrifugal microfluidic devices. , 2015, Talanta.

[55]  S. Harun,et al.  Biosensing enhancement of dengue virus using microballoon mixers on centrifugal microfluidic platforms. , 2015, Biosensors & bioelectronics.