Hyperspectral digital holographic microscopy approach for reduction of coherence induced disturbances in quantitative phase imaging of biological specimens

Coherence induced noise and parasitic reflections in the experimental setup are main restrictions that limit the resolution and measurement accuracy in laser light-based digital holographic microscopy (DHM). We explored, if coherence properties of partial coherent light sources can be mimicked by utilizing spectrally tunable lasers. Moreover, the performance for label-free quantitative phase imaging of biological specimens is illustrated utilizing an experimental configuration including a commercial microscope and tunable super continuum laser sources with a wavelength range of up to 230 nm.

[1]  Patrik Langehanenberg,et al.  Reduction of parasitic interferences in digital holographic microscopy by numerically decreased coherence length , 2012 .

[2]  Björn Kemper,et al.  Simplified approach for quantitative digital holographic phase contrast imaging of living cells. , 2011, Journal of biomedical optics.

[3]  F. Dubois,et al.  Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence. , 1999, Applied optics.

[4]  Steffi Ketelhut,et al.  Multi-spectral digital holographic microscopy for enhanced quantitative phase imaging of living cells , 2018, BiOS.

[5]  Patrik Langehanenberg,et al.  Multi-wavelength digital holographic microscopy for high-resolution inspection of surfaces and imaging of phase specimen , 2010, Photonics Europe.

[6]  T. Nomura,et al.  Image quality improvement of digital holography by superposition of reconstructed images obtained by multiple wavelengths. , 2008, Applied optics.

[7]  M. Mir,et al.  Simultaneous optical measurements of cell motility and growth , 2011, Biomedical optics express.

[8]  Jong Chul Ye,et al.  Self-reference quantitative phase microscopy for microfluidic devices. , 2010, Optics letters.

[9]  Gabriel Popescu,et al.  Fourier phase microscopy for investigation of biological structures and dynamics. , 2004, Optics letters.

[10]  D. Dirksen,et al.  Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging. , 2008, Applied optics.

[11]  O. Haeberlé,et al.  High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples. , 2009, Optics letters.

[12]  Daniel Carl,et al.  Parameter-optimized digital holographic microscope for high-resolution living-cell analysis. , 2004, Applied optics.

[13]  Patrik Langehanenberg,et al.  Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces , 2008 .

[14]  B. Kemper,et al.  Digital holographic microscopy for live cell applications and technical inspection. , 2008, Applied optics.

[15]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[16]  Daniel Carl,et al.  Investigation of living pancreas tumor cells by digital holographic microscopy. , 2006, Journal of biomedical optics.

[17]  Steffi Ketelhut,et al.  Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing. , 2016, Journal of visualized experiments : JoVE.

[18]  R. Dasari,et al.  Diffraction phase microscopy for quantifying cell structure and dynamics. , 2006, Optics letters.

[19]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[20]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[21]  Daniel Carl,et al.  Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells , 2006, SPIE Photonics Europe.

[22]  Gabriel Popescu,et al.  Hilbert phase microscopy for investigating fast dynamics in transparent systems. , 2005, Optics letters.

[23]  Natan T Shaked,et al.  Dual-interference-channel quantitative-phase microscopy of live cell dynamics. , 2009, Optics letters.

[24]  Tomasz Kozacki,et al.  Reconstruction of refractive-index distribution in off-axis digital holography optical diffraction tomographic system. , 2009, Optics express.

[25]  Natan T Shaked,et al.  Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics. , 2010, Journal of biomedical optics.

[26]  Huafeng Ding,et al.  Instantaneous Spatial Light Interference Microscopy. , 2010, Optics express.

[27]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[28]  Patrik Langehanenberg,et al.  Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy. , 2010, Journal of biomedical optics.

[29]  B. Wattellier,et al.  Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells. , 2009, Optics express.