Exploring the effects of different combinations of predictor variables for the treatment of wastewater by microalgae and biomass production

Abstract Microalgae provide an alternative and cost-efficient method for treating wastewater with simultaneous recovery of resources in the form of biomass. In order to increase the biomass productivity and wastewater treatment capability of microalgae, it is essential to provide the right combination of predictor variables. However, it is practically impossible to determine the best combination of variables through conventional methods from a large dataset of published research. Machine learning algorithms can easily detect the pattern in the large dataset and provide the best combination as desired. In the present analysis, decision tree algorithm was used to determine the effects and the best combination of predictor variables, including microalgae class, pre-cultivation stage deciding factors and operating variables, resulting in high biomass productivity and wastewater treatment capability. Decision tree analysis detected 10 different combinations of predictor variables leading to high nitrogen removal efficiency, 10 combinations for high phosphorus removal efficiency and 8 combinations for increased biomass production. These combinations were tested on recently published experimental findings and nearly 80% accuracy was obtained. The results obtained through machine learning analysis can be used in constructing high throughput experimental designs, which may assist in carrying out the efficient wastewater treatment at large scale.

[1]  G. Markou.  Fed-batch cultivation of Arthrospira and Chlorella in ammonia-rich wastewater: Optimization of nutrient removal and biomass production. , 2015, Bioresource technology.

[2]  M. Danquah,et al.  Cultivation of Nannochloropsis sp. microalgae in palm oil mill effluent (POME) media for phycoremediation and biomass production: Effect of microalgae cells with and without beads , 2020 .

[3]  Kisay Lee,et al.  Treatment of real wastewater using co-culture of immobilized Chlorella vulgaris and suspended activated sludge. , 2017, Water research.

[4]  Chen Wentao,et al.  Strategy study on enhancing lipid productivity of filamentous oleaginous microalgae Tribonema. , 2016, Bioresource technology.

[5]  Libin Yang,et al.  Nutrients recycling and biomass production from Chlorella pyrenoidosa culture using anaerobic food processing wastewater in a pilot-scale tubular photobioreactor. , 2020, Chemosphere.

[6]  Wen-rong Hu,et al.  Biomass production and nutrient assimilation by a novel microalga, Monoraphidium spp. SDEC-17, cultivated in a high-ammonia wastewater , 2016 .

[7]  Zhang Chunmin,et al.  Cultivation of Chlorella pyrenoidosa in soybean processing wastewater. , 2011, Bioresource technology.

[8]  Lei Zhao,et al.  Hydrogen and lipid production from starch wastewater by co-culture of anaerobic sludge and oleaginous microalgae with simultaneous COD, nitrogen and phosphorus removal. , 2015, Water research.

[9]  Wai Yan Cheah,et al.  Enhancing biomass and lipid productions of microalgae in palm oil mill effluent using carbon and nutrient supplementation , 2018 .

[10]  D. Tran,et al.  Simultaneous removal of pollutants and high value biomaterials production by Chlorella variabilis TH03 from domestic wastewater , 2020, Clean Technologies and Environmental Policy.

[11]  P. Show,et al.  Optimum interaction of light intensity and CO2 concentration in bioremediating N-rich real wastewater via assimilation into attached microalgal biomass as the feedstock for biodiesel production , 2020 .

[12]  Richa Kothari,et al.  Production of biodiesel from microalgae Chlamydomonas polypyrenoideum grown on dairy industry wastewater. , 2013, Bioresource technology.

[13]  R. Sen,et al.  Performance evaluation of microalgae for concomitant wastewater bioremediation, CO2 biofixation and lipid biosynthesis for biodiesel application , 2016 .

[14]  Z. Lei,et al.  Microalgal-bacterial aggregates for wastewater treatment: A mini-review , 2019 .

[15]  Sanjeev Kumar Prajapati,et al.  Development and performance evaluation of an algal biofilm reactor for treatment of multiple wastewaters and characterization of biomass for diverse applications. , 2017, Bioresource technology.

[16]  Paul Chen,et al.  The influence of microalgae on vegetable production and nutrient removal in greenhouse hydroponics , 2020 .

[17]  Xiaobo Tan,et al.  Continuous cultivation of Chlorella pyrenoidosa using anaerobic digested starch processing wastewater in the outdoors. , 2015, Bioresource technology.

[18]  Valeria Villanova,et al.  Autochthonous microalgae grown in municipal wastewaters as a tool for effectively removing nitrogen and phosphorous , 2020 .

[19]  Paul Chen,et al.  Cultivation of Chlorella vulgaris in a pilot-scale photobioreactor using real centrate wastewater with waste glycerol for improving microalgae biomass production and wastewater nutrients removal. , 2017, Bioresource technology.

[20]  M. Ballesteros,et al.  From piggery wastewater nutrients to biogas: Microalgae biomass revalorization through anaerobic digestion , 2016 .

[21]  Paul Chen,et al.  A hetero-photoautotrophic two-stage cultivation process to improve wastewater nutrient removal and enhance algal lipid accumulation. , 2012, Bioresource technology.

[22]  Hafiz M.N. Iqbal,et al.  Algal-based removal strategies for hazardous contaminants from the environment - A review. , 2019, The Science of the total environment.

[23]  J. Pires,et al.  Biotechnological potential of Synechocystis salina co-cultures with selected microalgae and cyanobacteria: Nutrients removal, biomass and lipid production. , 2016, Bioresource technology.

[24]  Enrica Uggetti,et al.  Assessment of the mechanisms involved in the removal of emerging contaminants by microalgae from wastewater: a laboratory scale study. , 2016, Journal of hazardous materials.

[25]  Yinghua Lu,et al.  NaCS-PDMDAAC immobilized autotrophic cultivation of Chlorella sp. for wastewater nitrogen and phosphate removal , 2012 .

[26]  B. Cheirsilp,et al.  Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. , 2012, Bioresource technology.

[27]  Zhenjiang Li,et al.  Advanced treatment of the low concentration petrochemical wastewater by Tribonema sp. microalgae grown in the open photobioreactors coupled with the traditional Anaerobic/Oxic process. , 2018, Bioresource technology.

[28]  F. Nan,et al.  Integration of wastewater treatment and flocculation for harvesting biomass for lipid production by a newly isolated self-flocculating microalga Scenedesmus rubescens SX , 2019 .

[29]  P. Show,et al.  Auto-flocculation through cultivation of Chlorella vulgaris in seafood wastewater discharge: Influence of culture conditions on microalgae growth and nutrient removal. , 2019, Journal of bioscience and bioengineering.

[30]  F. Nan,et al.  Nutrients removal from undiluted cattle farm wastewater by the two-stage process of microalgae-based wastewater treatment. , 2018, Bioresource technology.

[31]  Yongjun Zhao,et al.  Nutrient and heavy metal removal from piggery wastewater and CH4 enrichment in biogas based on microalgae cultivation technology under different initial inoculum concentration , 2019, Water environment research : a research publication of the Water Environment Federation.

[32]  N. Ren,et al.  A new lipid-rich microalga Scenedesmus sp. strain R-16 isolated using Nile red staining: effects of carbon and nitrogen sources and initial pH on the biomass and lipid production , 2013, Biotechnology for Biofuels.

[33]  A Seco,et al.  Microalgae cultivation in wastewater: nutrient removal from anaerobic membrane bioreactor effluent. , 2012, Bioresource technology.

[34]  Seonghwan Park,et al.  Comparison of batch cultivation strategies for cost-effective biomass production of Micractinium inermum NLP-F014 using a blended wastewater medium. , 2017, Bioresource technology.

[35]  Patrick J. McGinn,et al.  Assessment of the bioenergy and bioremediation potentials of the microalga Scenedesmus sp. AMDD cultivated in municipal wastewater effluent in batch and continuous mode , 2012 .

[36]  Xinhua Xu,et al.  Effect of carbon source on biomass growth and nutrients removal of Scenedesmus obliquus for wastewater advanced treatment and lipid production. , 2015, Bioresource technology.

[37]  B. Riaño,et al.  Treatment of agro-industrial wastewater using microalgae-bacteria consortium combined with anaerobic digestion of the produced biomass. , 2013, Bioresource technology.

[38]  V. Tyagi,et al.  Production of algal biomass for its biochemical profile using slaughterhouse wastewater for treatment under axenic conditions. , 2020, Bioresource technology.

[39]  Li-Hua Cheng,et al.  Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. , 2010, Bioresource technology.

[40]  B. Cheirsilp,et al.  Immobilized oleaginous microalgae for production of lipid and phytoremediation of secondary effluent from palm oil mill in fluidized bed photobioreactor. , 2017, Bioresource technology.

[41]  Shengjun Luo,et al.  Biomass and lipid production of marine microalgae using municipal wastewater and high concentration of CO2 , 2011 .

[42]  George Tchobanoglous,et al.  Wastewater Engineering: Treatment and Resource Recovery , 2013 .

[43]  M. C. Matsudo,et al.  Nitrogen supplementation for the production of Chlorella vulgaris biomass in secondary effluent from dairy industry , 2021 .

[44]  C. Lan,et al.  Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent. , 2011, Bioresource technology.

[45]  Lieyun Ding,et al.  Machine learning in construction: From shallow to deep learning , 2021 .

[46]  P. McGinn,et al.  Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD , 2013 .

[47]  S. Shanthakumar,et al.  Effect of culture conditions on biomass yield of acclimatized microalgae in ozone pre-treated tannery effluent: A simultaneous exploration of bioremediation and lipid accumulation potential. , 2020, Journal of environmental management.

[48]  Sebastián Sánchez,et al.  Biomass production of Scenedesmus obliquus from mixtures of urban and olive-oil mill wastewaters used as culture medium , 2013 .

[49]  Paul Chen,et al.  Growing wastewater-born microalga Auxenochlorella protothecoides UMN280 on concentrated municipal wastewater for simultaneous nutrient removal and energy feedstock production , 2012 .

[50]  Shijie Liu,et al.  Enhanced Microalgae Growth for Biodiesel Production and Nutrients Removal in Raw Swine Wastewater by Carbon Sources Supplementation , 2020, Waste and Biomass Valorization.

[51]  Wai Yan Cheah,et al.  Microalgae cultivation in palm oil mill effluent (POME) for lipid production and pollutants removal , 2018, Energy Conversion and Management.

[52]  Pascale Champagne,et al.  Nutrient removal, microalgal biomass growth, harvesting and lipid yield in response to centrate wastewater loadings. , 2016, Water research.

[53]  S. Burn,et al.  Evaluation of growth, nutrient utilization and production of bioproducts by a wastewater-isolated microalga. , 2013, Bioresource technology.

[54]  E. Cowling,et al.  The Nitrogen Cascade , 2003 .

[55]  Keerthi Katam,et al.  Improving the performance of activated sludge process with integrated algal biofilm for domestic wastewater treatment: System behavior during the start-up phase , 2021 .

[56]  Yanyan Su.  Revisiting carbon, nitrogen, and phosphorus metabolisms in microalgae for wastewater treatment. , 2020, The Science of the total environment.

[57]  Poonam Singh,et al.  Microalgal cultivation using aquaculture wastewater: Integrated biomass generation and nutrient remediation , 2017 .

[58]  A. Elazzazy,et al.  Reuse of shrimp farm wastewater as growth medium for marine microalgae isolated from Red Sea – Jeddah , 2018, Journal of Cleaner Production.

[59]  Xiangying Zhao,et al.  Lipids production and nutrients recycling by microalgae mixotrophic culture in anaerobic digestate of sludge using wasted organics as carbon source. , 2019, Bioresource technology.

[60]  H. Xiong,et al.  Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. , 2012, Bioresource technology.

[61]  J. Costa,et al.  Spirulina sp. as a Bioremediation Agent for Aquaculture Wastewater: Production of High Added Value Compounds and Estimation of Theoretical Biodiesel , 2020, BioEnergy Research.

[62]  I. de Godos,et al.  A comparative evaluation of microalgae for the degradation of piggery wastewater under photosynthetic oxygenation. , 2010, Bioresource technology.

[63]  Supriyanto,et al.  Artificial neural networks model for estimating growth of polyculture microalgae in an open raceway pond , 2019, Biosystems Engineering.

[64]  Hwai Chyuan Ong,et al.  Cultivation of Chlorella vulgaris using nutrients source from domestic wastewater for biodiesel production: Growth condition and kinetic studies , 2017 .

[65]  R. Sen,et al.  A biorefinery for valorization of industrial waste-water and flue gas by microalgae for waste mitigation, carbon-dioxide sequestration and algal biomass production. , 2019, The Science of the total environment.

[66]  A. Carlsson,et al.  Treatment of drainage solution from hydroponic greenhouse production with microalgae. , 2013, Bioresource technology.

[67]  Chih-Sheng Lin,et al.  Reduction of CO2 by a high-density culture of Chlorella sp. in a semicontinuous photobioreactor. , 2008, Bioresource technology.

[68]  Wenguang Zhou,et al.  Recycling nutrients from soy sauce wastewater to culture value-added Spirulina maxima , 2021 .

[69]  C. Ojha,et al.  Chlorella sorokiniana for integrated wastewater treatment, biomass accumulation and value-added product estimation under varying photoperiod regimes: A comparative study , 2021 .

[70]  G. Zeng,et al.  Responses of microalgae Coelastrella sp. to stress of cupric ions in treatment of anaerobically digested swine wastewater. , 2018, Bioresource technology.

[71]  D. Kang,et al.  Long-term outdoor cultivation by perfusing spent medium for biodiesel production from Chlorella minutissima. , 2010, Journal of bioscience and bioengineering.

[72]  Wei Wei,et al.  Comparative study of wastewater treatment and nutrient recycle via activated sludge, microalgae and combination systems. , 2016, Bioresource technology.

[73]  Siew-Moi Phang,et al.  Use of Chlorella vulgaris for bioremediation of textile wastewater. , 2010, Bioresource technology.

[74]  N. Ladommatos,et al.  Combined remediation and lipid production using Chlorella sorokiniana grown on wastewater and exhaust gases. , 2014, Bioresource technology.

[75]  Bilian Chen,et al.  Removal of nutrients from undiluted anaerobically treated piggery wastewater by improved microalgae. , 2016, Bioresource technology.

[76]  Brigitte Urban,et al.  Coupled nutrient removal and biomass production with mixed algal culture: impact of biotic and abiotic factors. , 2012, Bioresource technology.

[77]  B. Min,et al.  Microalgae-biorefinery with cascading resource recovery design associated to dairy wastewater treatment. , 2019, Bioresource technology.

[78]  N. Mallick,et al.  Carbon dioxide biofixation and lipid accumulation potential of an indigenous microalga Scenedesmus obliquus (Turpin) Kützing GA 45 for biodiesel production , 2016 .

[79]  Sokhee P. Jung,et al.  Enhanced biomass production through optimization of carbon source and utilization of wastewater as a nutrient source. , 2016, Journal of environmental management.

[80]  M. Martins,et al.  Strain screening and ozone pretreatment for algae farming in wastewaters from sugarcane ethanol biorefinery , 2020 .

[81]  Jun Zhu,et al.  Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. , 2010, Bioresource technology.

[82]  P. Show,et al.  Factors affecting pollutants removal and biomass production capability of Chlorella variabilis TH03 in domestic wastewater , 2020 .

[83]  M. Gutterres,et al.  Influence of period light on cultivation of microalgae consortium for the treatment of tannery wastewaters from leather finishing stage , 2020 .

[84]  C. González‐Fernández,et al.  Performance comparison of two photobioreactors configurations (open and closed to the atmosphere) treating anaerobically degraded swine slurry. , 2010, Bioresource technology.

[85]  J. Venkatesan,et al.  Marine Microalgae Biotechnology: Present Trends and Future Advances , 2015 .

[86]  Yun Huang,et al.  An annular photobioreactor with ion-exchange-membrane for non-touch microalgae cultivation with wastewater. , 2016, Bioresource technology.

[87]  L. Thomsen,et al.  Two-step process: Enhanced strategy for wastewater treatment using microalgae. , 2018, Bioresource technology.

[88]  H. Kariminia,et al.  Lipid production in mixotrophic cultivation of Chlorella vulgaris in a mixture of primary and secondary municipal wastewater , 2014 .

[89]  P. Anderberg,et al.  Machine learning and microsimulation techniques on the prognosis of dementia: A systematic literature review , 2017, PloS one.

[90]  A. Bertucco,et al.  Microalgae cultivation in high rate algal ponds using slaughterhouse wastewater for biofuel applications , 2016 .

[91]  Utilization of sugarcane factories’ wastes as inexpensive source of nutrients and CO2 for microalgal biomass production: process coupling and potential evaluation , 2021, SN Applied Sciences.

[92]  G. Raghavan,et al.  Effect of environmental factors on the biomass and lipid production of microalgae grown in wastewaters , 2019, Algal Research.

[93]  L. Chai,et al.  Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8 , 2013, Biotechnology for Biofuels.

[94]  K. Mohanty,et al.  Augmentation of native microalgae based biofuel production through statistical optimization of campus sewage wastewater as low-cost growth media , 2018, Journal of Environmental Chemical Engineering.

[95]  Kyung-Hyuk Lee,et al.  Evaluation of mixed oxidants generated by an electrochemical method , 2009 .

[96]  Pankaj Kumar,et al.  Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under the influence of phosphorus, pH and light intensities. , 2016, Bioresource technology.

[97]  Ji-ti Zhou,et al.  Minimizing greenhouse gas emission from wastewater treatment process by integrating activated sludge and microalgae processes. , 2020, The Science of the total environment.

[98]  Poonam Singh,et al.  Assessment of municipal wastewaters at various stages of treatment process as potential growth media for Chlorella sorokiniana under different modes of cultivation. , 2017, Bioresource technology.

[99]  P. García-Encina,et al.  Comparative evaluation of piggery wastewater treatment in algal-bacterial photobioreactors under indoor and outdoor conditions. , 2017, Bioresource technology.

[100]  Su-Hyun Han,et al.  The effects of wavelength and wavelength mixing ratios on microalgae growth and nitrogen, phosphorus removal using Scenedesmus sp. for wastewater treatment. , 2013, Bioresource technology.

[101]  J. Perales,et al.  Urban wastewater treatment by seven species of microalgae and an algal bloom: Biomass production, N and P removal kinetics and harvestability. , 2015, Water research.

[102]  Jyoti Singh,et al.  Characterization of microalga Scenedesmus sp. ISTGA1 for potential CO2 sequestration and biodiesel production , 2015 .

[103]  S. Venkata Mohan,et al.  Microalgae cultivation as tertiary unit operation for treatment of pharmaceutical wastewater associated with lipid production. , 2016, Bioresource technology.

[104]  P. Champagne,et al.  Centrate wastewater treatment with Chlorella vulgaris : Simultaneous enhancement of nutrient removal, biomass and lipid production , 2018, Chemical Engineering Journal.

[105]  Sanjeev Kumar Prajapati,et al.  An eco-friendly strategy for dairy wastewater remediation with high lipid microalgae-bacterial biomass production. , 2021, Journal of environmental management.

[106]  L. Delgadillo-Mirquez,et al.  Nitrogen and phosphate removal from wastewater with a mixed microalgae and bacteria culture , 2016, Biotechnology reports.

[107]  A. F. Ferreira,et al.  Microalgae biomass production using wastewater: Treatment and costs , 2016 .

[108]  M. Bravi,et al.  Microalgal biomass production by using ultra- and nanofiltration membrane fractions of olive mill wastewater. , 2013, Water research.

[109]  Sandhya Mishra,et al.  Microalgal biomass generation by phycoremediation of dairy industry wastewater: An integrated approach towards sustainable biofuel production. , 2016, Bioresource technology.

[110]  I. Thakur,et al.  An integrated approach for phycoremediation of wastewater and sustainable biodiesel production by green microalgae, Scenedesmus sp. ISTGA1 , 2019, Renewable Energy.

[111]  Shumei Gao,et al.  Performance of different microalgae-based technologies in biogas slurry nutrient removal and biogas upgrading in response to various initial CO2 concentration and mixed light-emitting diode light wavelength treatments , 2017 .

[112]  R. Luque,et al.  A review on sustainable microalgae based biofuel and bioenergy production: Recent developments , 2018 .

[113]  R. Borja,et al.  Heavy Metal Removal by Microalgae , 1999, Bulletin of environmental contamination and toxicology.

[114]  Byong-Hun Jeon,et al.  Cultivation of microalgae species in tertiary municipal wastewater supplemented with CO2 for nutrient removal and biomass production , 2013 .

[115]  Enhanced Arthrospira platensis Biomass Production Combined with Anaerobic Cattle Wastewater Bioremediation , 2021, Bioenergy research.

[116]  Y. Kitamura,et al.  Combination of brewery wastewater purification and CO2 fixation with potential value-added ingredients production via different microalgae strains cultivation , 2020 .

[117]  J. Takala,et al.  Scale-up potential of cultivating Chlorella zofingiensis in piggery wastewater for biodiesel production. , 2013, Bioresource technology.

[118]  Paul Chen,et al.  Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. , 2011, Bioresource technology.

[119]  Giovanni Antonio Lutzu,et al.  Latest developments in wastewater treatment and biopolymer production by microalgae , 2020 .

[120]  J. Pruvost,et al.  Microalgae cultivation in urban wastewater: Nutrient removal and biomass production for biodiesel and methane , 2015 .

[121]  Zhiquan Hu,et al.  Operation of a vertical algal biofilm enhanced raceway pond for nutrient removal and microalgae-based byproducts production under different wastewater loadings. , 2018, Bioresource technology.

[122]  H. Oh,et al.  Stepwise treatment of undiluted raw piggery wastewater, using three microalgal species adapted to high ammonia. , 2020, Chemosphere.

[123]  Manjinder Singh,et al.  Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters , 2011 .

[124]  H. Oh,et al.  Algae-bacteria interactions: Evolution, ecology and emerging applications. , 2016, Biotechnology advances.

[125]  Jo‐Shu Chang,et al.  Nutrients and COD removal of swine wastewater with an isolated microalgal strain Neochloris aquatica CL-M1 accumulating high carbohydrate content used for biobutanol production. , 2017, Bioresource technology.

[126]  Xiaobo Tan,et al.  Nutrients removal and lipids production by Chlorella pyrenoidosa cultivation using anaerobic digested starch wastewater and alcohol wastewater. , 2015, Bioresource technology.

[127]  Laura E. Walls,et al.  Non-sterile heterotrophic cultivation of native wastewater yeast and microalgae for integrated municipal wastewater treatment and bioethanol production , 2019, Biochemical Engineering Journal.

[128]  Yu Liu,et al.  COD capture: a feasible option towards energy self-sufficient domestic wastewater treatment , 2016, Scientific Reports.

[129]  F. G. Acién,et al.  Monoalgal and mixed algal cultures discrimination by using an artificial neural network , 2019, Algal Research.

[130]  B. Cheirsilp,et al.  Mitigation of carbon dioxide by oleaginous microalgae for lipids and pigments production: Effect of light illumination and carbon dioxide feeding strategies. , 2016, Bioresource technology.

[131]  H. Oh,et al.  Effects of photoperiod on nutrient removal, biomass production, and algal-bacterial population dynamics in lab-scale photobioreactors treating municipal wastewater. , 2015, Water research.

[132]  Qiang Hu,et al.  Handbook of microalgal culture , 2003 .

[133]  J. Umamaheswari,et al.  Outdoor cultivation of Chlorella pyrenoidosa in paddy-soaked wastewater and a feasibility study on biodiesel production from wet algal biomass through in-situ transesterification , 2020 .

[134]  P. Mondal,et al.  Phycoremediation of wastewater by Chlorella pyrenoidosa and utilization of its biomass for biogas production , 2021 .

[135]  M. Begam,et al.  Parboiled rice effluent: A wastewater niche for microalgae and cyanobacteria with growth coupled to comprehensive remediation and phosphorus biofertilization , 2016 .

[136]  Li Fen Wu,et al.  The feasibility of biodiesel production by microalgae using industrial wastewater. , 2012, Bioresource technology.

[137]  Yongjun Zhao,et al.  Performance of different microalgae-based technologies in nutrient removal and biogas upgrading in response to various GR24 concentrations , 2021 .

[138]  T. Stephenson,et al.  Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. , 2010, Bioresource technology.

[139]  A. Idris,et al.  Enhancing growth and lipid production of marine microalgae for biodiesel production via the use of different LED wavelengths. , 2014, Bioresource technology.

[140]  V. Mishra,et al.  Bioremediation of Nutrients and Heavy Metals from Wastewater by Microalgal Cells: Mechanism and Kinetics , 2019, Microbial Genomics in Sustainable Agroecosystems.

[141]  S. Velasquez-Orta,et al.  Wastewater-leachate treatment by microalgae: Biomass, carbohydrate and lipid production. , 2019, Ecotoxicology and environmental safety.

[142]  Haiying Wang,et al.  Application of ozonated piggery wastewater for cultivation of oil-rich Chlorella pyrenoidosa. , 2014, Bioresource technology.

[143]  T. Hasunuma,et al.  Optimizing real swine wastewater treatment efficiency and carbohydrate productivity of newly microalga Chlamydomonas sp. QWY37 used for cell-displayed bioethanol production. , 2020, Bioresource technology.

[144]  E. Zeng,et al.  Cultivation of oleaginous microalgae for removal of nutrients and heavy metals from biogas digestates , 2017 .

[145]  J. Umamaheswari,et al.  Phycoremediation of paddy-soaked wastewater by indigenous microalgae in open and closed culture system. , 2019, Journal of environmental management.

[146]  M. Iqbal,et al.  Microalgae an ecofriendly and sustainable wastewater treatment option: Biomass application in biofuel and bio-fertilizer production. A review , 2021 .

[147]  C. Barone,et al.  Wastewater treatment by microalgae can generate high quality biodiesel feedstock , 2017 .

[148]  M. Scarsella,et al.  Effect of Nitrogen and Phosphorus Starvations on Chlorella vulgaris Lipids Productivity and Quality under Different Trophic Regimens for Biodiesel Production , 2013 .

[149]  Yunlong Luo,et al.  Characterisation of microalgae-based monocultures and mixed cultures for biomass production and wastewater treatment , 2020 .

[150]  D. Los,et al.  Waste-free technology of wastewater treatment to obtain microalgal biomass for biodiesel production , 2017 .

[151]  A. Incharoensakdi,et al.  Removal of nutrients from domestic wastewater by microalgae coupled to lipid augmentation for biodiesel production and influence of deoiled algal biomass as biofertilizer for Solanum lycopersicum cultivation. , 2020, Chemosphere.

[152]  Jiang Wei,et al.  Microalgal cultivation in wastewater from the fermentation effluent in Riboflavin (B2) manufacturing for biodiesel production. , 2013, Bioresource technology.

[153]  Philip Owende,et al.  Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products , 2010 .

[154]  M. Calijuri,et al.  Innovative hybrid system for wastewater treatment: High-rate algal ponds for effluent treatment and biofilm reactor for biomass production and harvesting. , 2020, Journal of environmental management.

[155]  J. Zhao,et al.  Simultaneous wastewater treatment and lipid production by Scenedesmus sp. HXY2. , 2020, Bioresource technology.

[156]  G. Hodaifa,et al.  Influence of hydrodynamic stress in the growth of Scenedesmus obliquus using a culture medium based on olive-mill wastewater , 2010 .

[157]  K. Gao,et al.  Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae , 2005 .

[158]  Paul Chen,et al.  Local bioprospecting for high-lipid producing microalgal strains to be grown on concentrated municipal wastewater for biofuel production. , 2011, Bioresource technology.

[159]  G. González-Benito,et al.  Production of biomass by Spirulina maxima using sugar beet vinasse in growth media. , 2010, New biotechnology.

[160]  J. Perales,et al.  Capability of different microalgae species for phytoremediation processes: wastewater tertiary treatment, CO2 bio-fixation and low cost biofuels production. , 2014, Water research.

[161]  L. A. Daniel,et al.  Nutrient and pathogen removal from anaerobically treated black water by microalgae. , 2020, Journal of environmental management.

[162]  Poonam Singh,et al.  Heterotrophic cultivation of microalgae using aquaculture wastewater: A biorefinery concept for biomass production and nutrient remediation , 2017 .

[163]  C. Ugwu,et al.  Photobioreactors for mass cultivation of algae. , 2008, Bioresource technology.

[164]  Noor Irma Nazashida Mohd Hakimi,et al.  Potential of the microalgae-based integrated wastewater treatment and CO2 fixation system to treat Palm Oil Mill Effluent (POME) by indigenous microalgae; Scenedesmus sp. and Chlorella sp , 2019 .

[165]  G. Zeng,et al.  Nutrient removal and lipid production by Coelastrella sp. in anaerobically and aerobically treated swine wastewater. , 2016, Bioresource technology.

[166]  Frantisek Kastanek,et al.  Utilization of distillery stillage for energy generation and concurrent production of valuable microalgal biomass in the sequence: Biogas-cogeneration-microalgae-products , 2010 .

[167]  Li Wang,et al.  Corrigendum: The Serum Profile of Hypercytokinemia Factors Identified in H7N9-Infected Patients can Predict Fatal Outcomes , 2016, Scientific reports.

[168]  Wei-hong Jin,et al.  Effect of organic carbon to nitrogen ratio in wastewater on growth, nutrient uptake and lipid accumulation of a mixotrophic microalgae Chlorella sp. , 2019, Bioresource technology.

[169]  Sunja Cho,et al.  Reuse of effluent water from a municipal wastewater treatment plant in microalgae cultivation for biofuel production. , 2011, Bioresource technology.

[170]  Yi Tao,et al.  Application of effluent from WWTP in cultivation of four microalgae for nutrients removal and lipid production under the supply of CO2 , 2020 .

[171]  Md. Hasibur Rahaman,et al.  Optimization of biomass production and nutrients removal by Spirulina platensis from municipal wastewater. , 2017 .

[172]  Guo-jing Yang,et al.  Simultaneous nutrient removal and biomass/lipid production by Chlorella sp. in seafood processing wastewater. , 2018, The Science of the total environment.

[173]  Jo‐Shu Chang,et al.  Cultivating Chlorella sorokiniana AK-1 with swine wastewater for simultaneous wastewater treatment and algal biomass production. , 2020, Bioresource technology.

[174]  Y. Nancharaiah,et al.  Development and performance of halophilic microalgae-colonized aerobic granular sludge for treating seawater-based wastewater , 2020 .

[175]  P. García-Encina,et al.  Evaluation of the dynamics of microalgae population structure and process performance during piggery wastewater treatment in algal-bacterial photobioreactors. , 2018, Bioresource technology.

[176]  Fei Han,et al.  Growth of large-cell and easily-sedimentation microalgae Golenkinia SDEC-16 for biofuel production and campus sewage treatment , 2018, Renewable Energy.

[177]  C. Trick,et al.  Growth at Low Temperature Mimics High-Light Acclimation in Chlorella vulgaris , 1994, Plant physiology.

[178]  H. Haider,et al.  Wastewater treatment by microalgal membrane bioreactor: Evaluating the effect of organic loading rate and hydraulic residence time. , 2020, Journal of environmental management.

[179]  Lucie Novoveská,et al.  Optimizing microalgae cultivation and wastewater treatment in large-scale offshore photobioreactors , 2016 .

[180]  K. N. Sorokina,et al.  Screening and comparative metabolic profiling of high lipid content microalgae strains for application in wastewater treatment. , 2018, Bioresource technology.

[181]  F. G. Acién,et al.  Influence of pH and CO2 source on the performance of microalgae-based secondary domestic wastewater treatment in outdoors pilot raceways , 2015 .

[182]  A. Harvey,et al.  Biodiesel production from indigenous microalgae grown in wastewater. , 2014, Bioresource technology.

[183]  M. Farid,et al.  Potential of five different isolated colonial algal species for wastewater treatment and biomass energy production , 2017 .

[184]  Belina Ribeiro,et al.  Combining urban wastewater treatment with biohydrogen production--an integrated microalgae-based approach. , 2015, Bioresource technology.

[185]  Seyedeh Fatemeh Mohsenpour,et al.  Integrating micro-algae into wastewater treatment: A review. , 2021, The Science of the total environment.

[186]  Paul Chen,et al.  Auto-flocculation microalgae species Tribonema sp. and Synechocystis sp. with T-IPL pretreatment to improve swine wastewater nutrient removal. , 2020, The Science of the total environment.

[187]  C. S. Lin,et al.  Cultivation of oleaginous microalga Scenedesmus obliquus coupled with wastewater treatment for enhanced biomass and lipid production , 2019, Biochemical Engineering Journal.

[188]  Shu-lin Chen,et al.  Two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production. , 2012, Bioresource technology.

[189]  Jason A. Papin,et al.  Metabolic network reconstruction of Chlamydomonas offers insight into light-driven algal metabolism , 2011, Molecular systems biology.

[190]  H. Ngo,et al.  Co-culture of microalgae-activated sludge for wastewater treatment and biomass production: Exploring their role under different inoculation ratios. , 2020, Bioresource technology.

[191]  G. Shurson,et al.  Growing Chlorella sp. on meat processing wastewater for nutrient removal and biomass production. , 2015, Bioresource technology.

[192]  N. Moheimani,et al.  Biomass production of marine microalga Tetraselmis suecica using biogas and wastewater as nutrients , 2021 .

[193]  S. Venkata Mohan,et al.  Heterotrophic microalgae cultivation to synergize biodiesel production with waste remediation: progress and perspectives. , 2015, Bioresource technology.

[194]  Longlong Ma,et al.  Cultivation of microalgae Chlorella zofingiensis on municipal wastewater and biogas slurry towards bioenergy. , 2018, Journal of bioscience and bioengineering.

[195]  Paul Chen,et al.  Integration of algae cultivation as biodiesel production feedstock with municipal wastewater treatment: strains screening and significance evaluation of environmental factors. , 2011, Bioresource technology.

[196]  Jo‐Shu Chang,et al.  Perspectives on microalgal CO₂-emission mitigation systems--a review. , 2011, Biotechnology advances.

[197]  J. Perales,et al.  Performance of a flat panel reactor in the continuous culture of microalgae in urban wastewater: prediction from a batch experiment. , 2013, Bioresource technology.

[198]  Animesh Dutta,et al.  Integrated Haematococcus pluvialis biomass production and nutrient removal using bioethanol plant waste effluent , 2017 .

[199]  D. Leung,et al.  Cultivation of Spirulina platensis for biomass production and nutrient removal from synthetic human urine , 2013 .

[200]  I. Ferrer,et al.  Integrating microalgae tertiary treatment into activated sludge systems for energy and nutrients recovery from wastewater. , 2018, Bioresource technology.

[201]  R. N. Singh,et al.  Development of suitable photobioreactor for algae production – A review , 2012 .

[202]  Wu Yin-hu,et al.  Culture of Scenedesmus sp. LX1 in the modified effluent of a wastewater treatment plant of an electric factory by photo-membrane bioreactor. , 2011, Bioresource technology.

[203]  Willy Verstraete,et al.  Decentralized two-stage sewage treatment by chemical-biological flocculation combined with microalgae biofilm for nutrient immobilization in a roof installed parallel plate reactor. , 2013, Bioresource technology.

[204]  A. Bhatnagar,et al.  Versatile applications of freshwater and marine water microalgae in dairy wastewater treatment, lipid extraction and tetracycline biosorption. , 2018, Bioresource technology.

[205]  Guanyi Chen,et al.  A novel concept of bicarbonate-carbon utilization via an absorption-microalgae hybrid process assisted with nutrient recycling from soybean wastewater , 2019, Journal of Cleaner Production.

[206]  Y. Bashan,et al.  Heterotrophic cultures of microalgae: metabolism and potential products. , 2011, Water research.

[207]  G. Zeng,et al.  Continuous microalgae cultivation in aquaculture wastewater by a membrane photobioreactor for biomass production and nutrients removal , 2016 .

[208]  Tsuyoshi Tanaka,et al.  Selection and characterization of microalgae with potential for nutrient removal from municipal wastewater and simultaneous lipid production. , 2020, Journal of bioscience and bioengineering.

[209]  V. Himabindu,et al.  Phycoremediation of sewage wastewater and industrial flue gases for biomass generation from microalgae , 2018, South African Journal of Chemical Engineering.

[210]  J. Ometto,et al.  Microalgae-mediated bioremediation and valorization of cattle wastewater previously digested in a hybrid anaerobic reactor using a photobioreactor: Comparison between batch and continuous operation. , 2018, The Science of the total environment.

[211]  B. Riaño,et al.  Microalgal-based systems for wastewater treatment: Effect of applied organic and nutrient loading rate on biomass composition , 2012 .

[212]  Feng Chen,et al.  Heterotrophic production of lutein by selected Chlorella strains , 1997, Journal of Applied Phycology.

[213]  Meilin He,et al.  Bioremediation of Pyropia-processing wastewater coupled with lipid production using Chlorella sp. , 2020, Bioresource technology.

[214]  A. Richmond,et al.  Biological Principles of Mass Cultivation , 2007 .

[215]  M. Erdem Günay,et al.  Constructing global models from past publications to improve design and operating conditions for direct alcohol fuel cells , 2016 .

[216]  Joo-Youp Lee,et al.  Removal of Ammonia from Wastewater Effluent by Chlorella Vulgaris , 2010 .

[217]  Poonam Singh,et al.  Prospects, recent advancements and challenges of different wastewater streams for microalgal cultivation. , 2017, Journal of environmental management.

[218]  J. Costa,et al.  Spirulina sp. LEB 18 cultivation in a raceway-type bioreactor using wastewater from desalination process: Production of carbohydrate-rich biomass. , 2020, Bioresource technology.

[219]  D. Das,et al.  Production of microbial biomass feedstock via co-cultivation of microalgae-bacteria consortium coupled with effective wastewater treatment: A sustainable approach , 2019, Algal Research.

[220]  Teresa M Mata,et al.  Parametric study of a brewery effluent treatment by microalgae Scenedesmus obliquus. , 2012, Bioresource technology.

[221]  Paul Chen,et al.  Culture of Microalgae Chlamydomonas reinhardtii in Wastewater for Biomass Feedstock Production , 2010, Applied biochemistry and biotechnology.

[222]  Q. Lu,et al.  Does exogenous carbon source always promote algal biomass and nutrients removal in algal-bacterial wastewater remediation? , 2021 .

[223]  G. Buitrón,et al.  Influence of solar irradiance levels on the formation of microalgae-bacteria aggregates for municipal wastewater treatment , 2017 .

[224]  Keerthi Katam,et al.  Simultaneous treatment of domestic wastewater and bio-lipid synthesis using immobilized and suspended cultures of microalgae and activated sludge , 2019, Journal of Industrial and Engineering Chemistry.

[225]  S. Hena,et al.  Cultivation of algae consortium in a dairy farm wastewater for biodiesel production , 2015 .

[226]  Yanna Liang,et al.  Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions , 2009, Biotechnology Letters.

[227]  Xiaonan Wang,et al.  Machine learning prediction of biochar yield and carbon contents in biochar based on biomass characteristics and pyrolysis conditions. , 2019, Bioresource technology.

[228]  R. Ruan,et al.  Magnetic field intervention on growth of the filamentous microalgae Tribonema sp. in starch wastewater for algal biomass production and nutrients removal: Influence of ambient temperature and operational strategy. , 2020, Bioresource technology.

[229]  Alireza Moosavi Zenooz,et al.  Comparison of different artificial neural network architectures in modeling of Chlorella sp. flocculation , 2017, Preparative biochemistry & biotechnology.

[230]  I. de Godos,et al.  A systematic comparison of the potential of microalgae-bacteria and purple phototrophic bacteria consortia for the treatment of piggery wastewater. , 2019, Bioresource technology.

[231]  S. Lam,et al.  Treatment of African catfish, Clarias gariepinus wastewater utilizing phytoremediation of microalgae, Chlorella sp. with Aspergillus niger bio-harvesting. , 2015, Bioresource technology.

[232]  J. C. Goldman.  Temperature effects on phytoplankton growth in continuous culture1 , 1977 .

[233]  Zheng Zheng,et al.  Effects of various LED light wavelengths and light intensity supply strategies on synthetic high-strength wastewater purification by Chlorella vulgaris , 2013, Biodegradation.

[234]  P. Bakonyi,et al.  Evaluation of gradual adaptation of mixed microalgae consortia cultivation using textile wastewater via fed batch operation , 2018, Biotechnology reports.

[235]  Iracema Andrade Nascimento,et al.  Comparing the use of different domestic wastewaters for coupling microalgal production and nutrient removal. , 2013, Bioresource technology.

[236]  Paul Chen,et al.  Co-culture of Chlorella and wastewater-borne bacteria in vinegar production wastewater: Enhancement of nutrients removal and influence of algal biomass generation , 2020 .

[237]  J. Vargas,et al.  Microalgae derived biomass and bioenergy production enhancement through biogas purification and wastewater treatment , 2021 .

[238]  Yi Wang,et al.  Integrating acidogenic fermentation and microalgae cultivation of bacterial-algal coupling system for mariculture wastewater treatment. , 2020, Bioresource technology.

[239]  M. T. O. Ledesma,et al.  Carbon, nitrogen, and phosphorus removal, and lipid production by three saline microalgae grown in synthetic wastewater irradiated with different photon fluxes , 2018 .

[240]  F. Almomani,et al.  Performance Of Chlorella Vulgaris, Neochloris Oleoabundans, and mixed indigenous microalgae for treatment of primary effluent, secondary effluent and centrate , 2016 .

[241]  Katarzyna Chojnacka,et al.  Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures , 2004 .

[242]  F. G. Fernández,et al.  Utilization of centrate from wastewater treatment for the outdoor production of Nannochloropsis gaditana biomass at pilot-scale , 2015 .

[243]  D. Batstone,et al.  Simultaneous treatment and single cell protein production from agri-industrial wastewaters using purple phototrophic bacteria or microalgae - A comparison. , 2018, Bioresource technology.

[244]  J. Pires,et al.  The effect of increasing CO2 concentrations on its capture, biomass production and wastewater bioremediation by microalgae and cyanobacteria , 2016 .

[245]  M. E. Günay,et al.  Exploring the critical factors of algal biomass and lipid production for renewable fuel production by machine learning , 2021 .

[246]  Hyun-Chul Kim,et al.  Microalgal species growing on piggery wastewater as a valuable candidate for nutrient removal and biodiesel production. , 2013, Journal of environmental management.

[247]  S. M. D. A. G. Souza,et al.  Assessing the potential of microalgae for nutrients removal from a landfill leachate using an innovative tubular photobioreactor , 2020 .

[248]  Guixia Ma,et al.  Integrated campus sewage treatment and biomass production by Scenedesmus quadricauda SDEC-13. , 2015, Bioresource technology.

[249]  A. A. H. Khalid,et al.  Assessing the feasibility of microalgae cultivation in agricultural wastewater: The nutrient characteristics , 2019, Environmental Technology & Innovation.

[250]  M. Işık,et al.  Optimization of membrane photobioreactor; the effect of hydraulic retention time on biomass production and nutrient removal by mixed microalgae culture , 2020 .

[251]  R. Lovitt,et al.  Integrated production of long chain polyunsaturated fatty acids (PUFA)-rich Schizochytrium biomass using a nutrient supplemented marine aquaculture wastewater , 2010 .

[252]  Y. Liu,et al.  Biomass production and nutrients removal by a new microalgae strain Desmodesmus sp. in anaerobic digestion wastewater. , 2014, Bioresource technology.

[253]  L. Ferro,et al.  Isolation and characterization of microalgal strains for biomass production and wastewater reclamation in Northern Sweden , 2018 .

[254]  D. Tran,et al.  Semi-continuous removal of nutrients and biomass production from domestic wastewater in raceway reactors using Chlorella variabilis TH03-bacteria consortia , 2020 .

[255]  I. Ferrer,et al.  The effect of primary treatment of wastewater in high rate algal pond systems: Biomass and bioenergy recovery. , 2019, Bioresource technology.

[256]  C. Soccol,et al.  Screening of microalgae with potential for biodiesel production and nutrient removal from treated domestic sewage , 2011 .

[257]  H. Jia,et al.  Nitrogen removal in photo sequence batch reactor using algae-bacteria consortium , 2018, Journal of Water Process Engineering.

[258]  Yulie E. Meneses,et al.  Integration of sodium hypochlorite pretreatment with co-immobilized microalgae/bacteria treatment of meat processing wastewater. , 2020, Bioresource technology.

[259]  P. Broady,et al.  The effects of CO₂ addition along a pH gradient on wastewater microalgal photo-physiology, biomass production and nutrient removal. , 2015, Water research.

[260]  Abhishek Guldhe,et al.  Wastewater to biofuels: Comprehensive evaluation of various flocculants on biochemical composition and yield of microalgae , 2018, Ecological Engineering.

[261]  Yuguang Zhou,et al.  Towards high-quality biodiesel production from microalgae using original and anaerobically-digested livestock wastewater. , 2020, Chemosphere.

[262]  Sanjeev Kumar Prajapati,et al.  Screening native microalgal consortia for biomass production and nutrient removal from rural wastewaters for bioenergy applications , 2016 .

[263]  K. Gao,et al.  Effects of CO2 concentrations on the freshwater microalgae, Chlamydomonas reinhardtii, Chlorella pyrenoidosa and Scenedesmus obliquus (Chlorophyta) , 2003, Journal of Applied Phycology.

[264]  H. Pei,et al.  Inclined algal biofilm photobioreactor (IABPBR) for cost-effective cultivation of lipid-rich microalgae and treatment of seawater-diluted anaerobically digested effluent from kitchen waste with the aid of phytohormones. , 2020, Bioresource technology.

[265]  S. Brar,et al.  Microalgae cultivation in thin stillage anaerobic digestate for nutrient recovery and bioproduct production , 2020 .

[266]  C. Lan,et al.  CO2 bio-mitigation using microalgae , 2008, Applied Microbiology and Biotechnology.

[267]  G. Spatafora,et al.  Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production , 2011 .

[268]  Paul Chen,et al.  Microalgae-based wastewater treatment for nutrients recovery: A review. , 2019, Bioresource technology.

[269]  E. Bouwer,et al.  Effects of inoculum size, light intensity, and dose of anaerobic digestion centrate on growth and productivity of Chlorella and Scenedesmus microalgae and their poly-culture in primary and secondary wastewater , 2016 .

[270]  M. Kornaros,et al.  Sequential cultivation of microalgae in raw and recycled dairy wastewater: Microalgal growth, wastewater treatment and biochemical composition. , 2019, Bioresource technology.

[271]  A. Konopka,et al.  Effect of Temperature on Blue-Green Algae (Cyanobacteria) in Lake Mendota , 1978, Applied and environmental microbiology.

[272]  Ganti S. Murthy,et al.  Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review , 2013 .

[273]  Richard T. Sayre,et al.  Microalgae: The Potential for Carbon Capture , 2010 .

[274]  Tonghui Xie,et al.  Swine digestate treatment by prior nitrogen-starved Chlorella vulgaris: The effect of over-compensation strategy on microalgal biomass production and nutrient removal. , 2021, The Science of the total environment.

[275]  Jo‐Shu Chang,et al.  Simultaneous microalgal biomass production and CO2 fixation by cultivating Chlorella sp. GD with aquaculture wastewater and boiler flue gas. , 2016, Bioresource technology.

[276]  G. S. Rolim,et al.  Influence of light intensity and tannery wastewater concentration on biomass production and nutrient removal by microalgae Scenedesmus sp. , 2017 .

[277]  S. Venkata Mohan,et al.  Heterotrophic cultivation of mixed microalgae for lipid accumulation and wastewater treatment during sequential growth and starvation phases: Effect of nutrient supplementation , 2012 .

[278]  L. Nghiem,et al.  A hybrid anaerobic and microalgal membrane reactor for energy and microalgal biomass production from wastewater , 2020 .

[279]  B. Riaño,et al.  Treatment of fish processing wastewater with microalgae-containing microbiota. , 2011, Bioresource technology.

[280]  Fangyuan Yu,et al.  A comparative study on growth characters and nutrients removal from wastewater by two microalgae under optimized light regimes , 2020 .

[281]  L. A. Daniel,et al.  Microalgae cultivation for municipal and piggery wastewater treatment in Brazil , 2019, Journal of Water Process Engineering.

[282]  S. Huo,et al.  Filamentous microalgae Tribonema sp. cultivation in the anaerobic/oxic effluents of petrochemical wastewater for evaluating the efficiency of recycling and treatment , 2019, Biochemical Engineering Journal.

[283]  Byung-Gon Ryu,et al.  Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity. , 2013, Bioresource technology.

[284]  Zhenhong Yuan,et al.  Treatment of low C/N ratio wastewater and biomass production using co-culture of Chlorella vulgaris and activated sludge in a batch photobioreactor. , 2019, Bioresource technology.

[285]  Chengwu Zhang,et al.  Resourceful treatment of cane sugar industry wastewater by Tribonema minus towards the production of valuable biomass. , 2020, Bioresource technology.

[286]  Y. Kitamura,et al.  Microalgae carbon fixation integrated with organic matters recycling from soybean wastewater: Effect of pH on the performance of hybrid system. , 2020, Chemosphere.

[287]  J. Costa,et al.  Spirulina sp. LEB 18 cultivation in outdoor pilot scale using aquaculture wastewater: High biomass, carotenoid, lipid and carbohydrate production , 2020 .

[288]  J. Takala,et al.  Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. , 2013, Water research.