Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut

Abstract Three class-specific monoclonal antibodies against aflatoxins were screened by a designed strategy in which aflatoxin G 2 was used as competitor in the screening ELISA system. With a high cross-reactivity (65%) to aflatoxin G 2 , antibody 10C9 had the most similar sensitivity for five aflatoxins (AFB 1 , AFB 2 , AFG 1 , AFG 2 and AFM 1 ), whose I 50 values were in a range of 2.1–3.2 ng ml −1 . So, antibody 10C9 was selected to develop an ELISA for determination of aflatoxin B 1 , B 2 , G 1 , G 2 and total of them in peanut samples. And spiked recoveries were from 87.5% to 102.0%. The results indicate that the ELISA developed can accurately determine total aflatoxins in samples of peanuts after the simple and rapid extraction procedure.

[1]  A. Magnoli,et al.  Commercial bentonites as detoxifier of broiler feed contaminated with aflatoxin , 2008 .

[2]  Lisa C Shriver-Lake,et al.  Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor. , 2006, Biosensors & bioelectronics.

[3]  B. Hammock,et al.  Development of a class selective immunoassay for the type II pyrethroid insecticides , 2005 .

[4]  Xiao Liang,et al.  Development of a sensitive ELISA for the analysis of the organophosphorous insecticide fenthion in fruit samples , 2008 .

[5]  J. M. Soriano,et al.  Presence of aflatoxin M1 in pasteurized milk from Morocco. , 2007, International journal of food microbiology.

[6]  K. Ahn,et al.  Hapten heterology for a specific and sensitive indirect enzyme-linked immunosorbent assay for organophosphorus insecticide fenthion. , 2007, Analytica chimica acta.

[7]  A. Pfohl-Leszkowicz,et al.  Occurrence of aflatoxin B1, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam , 2007 .

[8]  C. Milstein,et al.  [1] Preparation of monoclonal antibodies: Strategies and procedures , 1981 .

[9]  A. Formigoni,et al.  High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese. , 2005, Journal of chromatography. A.

[10]  Gathumbi,et al.  Production of ultrasensitive antibodies against aflatoxin B1 , 2001, Letters in applied microbiology.

[11]  Reinhard Niessner,et al.  Comparison of hybridoma screening methods for the efficient detection of high-affinity hapten-specific monoclonal antibodies. , 2008, Journal of immunological methods.

[12]  J. Smith,et al.  Aflatoxin monoclonals: academic development to commercial production , 1990, Letters in Applied Microbiology.

[13]  S. Ding,et al.  Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds. , 2007, Analytical chemistry.

[14]  Á. Móricz,et al.  Overpressured layer chromatographic determination of aflatoxin B1, B2, G1 and G2 in red paprika , 2007 .

[15]  G. Raab.  Comparison of a logistic and a mass-action curve for radioimmunoassay data. , 1983, Clinical chemistry.

[16]  G. Massolini,et al.  Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions. , 2007, Journal of pharmaceutical and biomedical analysis.

[17]  P Delfosse,et al.  Production and characterization of monoclonal antibodies for aflatoxin B1 , 1999, Letters in applied microbiology.

[18]  Bulent Kabak,et al.  Survey of aflatoxin B1 in helva, a traditional Turkish food, by TLC , 2007 .

[19]  M. Wilkinson,et al.  A survey of the incidence and level of aflatoxin contamination in a range of imported spice preparations on the Irish retail market , 2008 .

[20]  H. Ayçiçek,et al.  Determination of aflatoxin levels in some dairy and food products which consumed in Ankara, Turkey , 2005 .

[21]  K. Ehrlich,et al.  Aflatoxin-producing Aspergillus species from Thailand. , 2007, International journal of food microbiology.

[22]  John Davis Groopman,et al.  The toxicology of aflatoxins: human health, veterinary and agricultural significance. , 1993 .

[23]  J. Beatty,et al.  Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. , 1987, Journal of immunological methods.

[24]  J. Groopman,et al.  High-affinity monoclonal antibodies for aflatoxins and their application to solid-phase immunoassays. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[25]  J. Hernández-Hierro,et al.  Potential of near infrared spectroscopy for the analysis of mycotoxins applied to naturally contaminated red paprika found in the Spanish market. , 2008, Analytica chimica acta.