Seed-borne Probiotic Yeasts Foster Plant Growth and Elicit Health Protection in Black Gram (Vigna mungo L.)

Black gram is one of the most indispensable components of the world food basket and the growth and health of the crop get influenced by biotic and abiotic factors. Beneficial phyto-microbes are one among them that influence the crop growth, more particularly the seed borne microbes are comparatively beneficial, that they pass from generation to generation and are associated with the plants from establishment to development. In the present study, twenty seed-borne yeasts were characterized and tested for growth promotion of black gram and their antagonism against black gram phytopathogens. Two yeasts, Pichia kudriavzevii POY5 and Issatchenkia terricola GRY4, produced indole acetic acid (IAA), siderophore, 1-amino cyclopropane-1-carboxylic acid deaminase (ACCD), and plant defense enzymes. They solubilized phosphate and zinc and fixed atmospheric nitrogen. Inoculation of these two yeast isolates and Rhizobium BMBS1 improved the seed germination, physiological parameters and yield of black gram. Inoculation of Rhizoctonia solani-challenged plants with plant growth-promoting yeasts, resulted in the synthesis of defense-related enzymes such as peroxidases (POD), chitinases, catalase (CAT), and polyphenol oxidases (PPO). Thus, the seed-borne yeasts, Pichia kudriavzevii POY5 and Issatchenkia terricola GRY4, could be used as plant probiotics for black gram.

[1]  Komal A. Chandarana,et al.  Interaction between ciliate and plant growth promoting bacteria influences the root structure of rice plants, soil PLFAs and respiration properties , 2021, Rhizosphere.

[2]  K. Ragunath,et al.  Prospects of utilizing a multifarious yeast (MSD1), isolated from South Indian coast as an agriculture input , 2021, Biocatalysis and Agricultural Biotechnology.

[3]  H. E. El Enshasy,et al.  Production of Plant Beneficial and Antioxidants Metabolites by Klebsiella variicola under Salinity Stress , 2021, Molecules.

[4]  H. E. El Enshasy,et al.  Bacterial Plant Biostimulants: A Sustainable Way towards Improving Growth, Productivity, and Health of Crops , 2021, Sustainability.

[5]  A. Basu,et al.  Plant Growth Promoting Rhizobacteria (PGPR) as Green Bioinoculants: Recent Developments, Constraints, and Prospects , 2021, Sustainability.

[6]  S. Uthandi,et al.  Co- inoculant response of plant growth promoting non-rhizobial endophytic yeast Candida tropicalis VYW1 and Rhizobium sp. VRE1 for enhanced plant nutrition, nodulation, growth and soil nutrient status in Mungbean (Vigna mungo L.,) , 2020 .

[7]  H. Yasmin,et al.  Effect of 24-Epibrassinolide on plant growth, antioxidants defense system and endogenous hormones in two wheat varieties under drought stress. , 2020, Physiologia plantarum.

[8]  P. Ramteke,et al.  ACC deaminase and antioxidant enzymes producing halophilic Enterobacter sp. PR14 promotes the growth of rice and millets under salinity stress , 2020, Physiology and Molecular Biology of Plants.

[9]  J. Kumla,et al.  Evaluation of Multifarious Plant Growth Promoting Trials of Yeast Isolated from the Soil of Assam Tea (Camellia sinensis var. assamica) Plantations in Northern Thailand , 2020, Microorganisms.

[10]  S. Uthandi,et al.  Non-rhizobial endophytic (NRE) yeasts assist nodulation of Rhizobium in root nodules of blackgram (Vigna mungo L.) , 2020, Archives of Microbiology.

[11]  F. J. Corpas,et al.  Plant catalases as NO and H2S targets , 2020, Redox biology.

[12]  G. S. El-Sayyad,et al.  The effective antagonistic potential of plant growth-promoting rhizobacteria against Alternaria solani-causing early blight disease in tomato plant , 2020 .

[13]  M. Ancín,et al.  Plant growth-promoting traits of yeasts isolated from Spanish vineyards: benefits for seedling development. , 2020, Microbiological research.

[14]  A. Rathore,et al.  Morphological and molecular characterization of Macrophomina phaseolina isolated from three legume crops and evaluation of mungbean genotypes for resistance to dry root rot , 2020 .

[15]  C. Lucas,et al.  Potential of Yeasts as Biocontrol Agents of the Phytopathogen Causing Cacao Witches’ Broom Disease: Is Microbial Warfare a Solution? , 2019, Front. Microbiol..

[16]  R. Sayyed,et al.  Siderophore production in groundnut rhizosphere isolate, Achromobacter sp. RZS2 influenced by physicochemical factors and metal ions , 2019, Environmental Sustainability.

[17]  Anna Chailyan,et al.  Multi-omics characterization of the necrotrophic mycoparasite Saccharomycopsis schoenii , 2019, PLoS pathogens.

[18]  D. Laranjeira,et al.  Biological control of Rhizoctonia solani in cowpea plants using yeast , 2019, Tropical Plant Pathology.

[19]  C. Gostinčar,et al.  Stress-Tolerant Yeasts: Opportunistic Pathogenicity Versus Biocontrol Potential , 2019, Genes.

[20]  A. Thammasittirong,et al.  Antifungal activity of soil yeast (Lachancea kluyveri SP132) against rice pathogenic fungi and its plant growth promoting activity. , 2019 .

[21]  J. Larsen,et al.  Plant growth promotion traits of rhizosphere yeasts and their response to soil characteristics and crop cycle in maize agroecosystems , 2018, Rhizosphere.

[22]  E. Thines,et al.  Yeasts from Different Habitats and Their Potential as Biocontrol Agents , 2018 .

[23]  S. Doty.  Symbiotic Plant-Bacterial Endospheric Interactions , 2018, Microorganisms.

[24]  C. Bradley,et al.  Rhizoctonia solani: taxonomy, population biology and management of rhizoctonia seedling disease of soybean , 2022, Plant Pathology.

[25]  S. Doty,et al.  Endophytic Yeasts: Biology, Ecology and Applications , 2018 .

[26]  T. Ichor,et al.  Isolation and Characterization of Hydrocarbon Utilizing Yeast (HUY) Isolates from Palm Wine , 2016 .

[27]  W. Fang,et al.  Plant growth-promoting traits of yeasts isolated from the phyllosphere and rhizosphere of Drosera spatulata Lab. , 2016, Fungal biology.

[28]  P. Masniyom,et al.  Isolation of yeast and acetic acid bacteria from palmyra palm fruit pulp (Borassus flabellifer Linn.). , 2016 .

[29]  R. Berzhanova,et al.  Plant growth-promoting and antifungal activity of yeasts from dark chestnut soil. , 2015, Microbiological research.

[30]  P. Somta,et al.  Genetic diversity of the black gram [Vigna mungo (L.) Hepper] gene pool as revealed by SSR markers , 2015, Breeding science.

[31]  S. Limtong,et al.  Indole-3-acetic acid production by newly isolated red yeast Rhodosporidium paludigenum. , 2015, The Journal of general and applied microbiology.

[32]  W. Fang,et al.  Indole-3-Acetic Acid-Producing Yeasts in the Phyllosphere of the Carnivorous Plant Drosera indica L , 2014, PloS one.

[33]  S. Limtong,et al.  Plant growth-promoting traits of epiphytic and endophytic yeasts isolated from rice and sugar cane leaves in Thailand. , 2014, Fungal biology.

[34]  M. R. Espuny,et al.  Plant growth promotion in cereal and leguminous agricultural important plants: from microorganism capacities to crop production. , 2014, Microbiological research.

[35]  W. Okunowo,et al.  Isolation and characterization of yeast strains from local food crops , 2013 .

[36]  C. Young,et al.  Phosphate-solubilizing soil yeast Meyerozyma guilliermondii CC1 improves maize (Zea mays L.) productivity and minimizes requisite chemical fertilization , 2013, Plant and Soil.

[37]  Hien Thanh Nguyen,et al.  Plant growth promoting characteristics of soil yeast (Candida tropicalis HY) and its effectiveness for promoting rice growth , 2012 .

[38]  Young Cheol Kim,et al.  Enhancement of Plant Drought Tolerance by Microbes , 2012 .

[39]  S. Ghosh.  Study of yeast flora from fruit of Syzygium cumini (linn) skeel , 2011 .

[40]  A. Botha.  The importance and ecology of yeasts in soil , 2011 .

[41]  C. Kurtzman,et al.  Methods for isolation, phenotypic characterization and maintenance of yeasts , 2011 .

[42]  R. Schwan,et al.  Identification and characterization of yeasts in sugarcane silages , 2010, Journal of applied microbiology.

[43]  S. Lumyong,et al.  Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils , 2010 .

[44]  S. Doty,et al.  Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees. , 2009, Mycological research.

[45]  C. Ryu,et al.  Rhizosphere bacteria help plants tolerate abiotic stress. , 2009, Trends in plant science.

[46]  A. Botha,et al.  Evidence of Symbiosis Between the Soil Yeast Cryptococcus laurentii and a Sclerophyllous Medicinal Shrub, Agathosma betulina (Berg.) Pillans , 2009, Microbial Ecology.

[47]  D. Kleiner,et al.  Spores of the mycorrhizal fungus Glomus mosseae host yeasts that solubilize phosphate and accumulate polyphosphates , 2008, Mycorrhiza.

[48]  J. Michiels,et al.  Physiological and genetic analysis of root responsiveness to auxin-producing plant growth-promoting bacteria in common bean (Phaseolus vulgaris L.) , 2007, Plant and Soil.

[49]  K. El-Tarabily,et al.  Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots , 2005, Biology and Fertility of Soils.

[50]  M. D. Tosetti,et al.  Control of Botrytis cinerea strains resistant to iprodione in apple with rhodotorulic acid and yeasts , 2005 .

[51]  K. El-Tarabily,et al.  Suppression of Rhizoctonia solani diseases of sugar beet by antagonistic and plant growth‐promoting yeasts , 2004, Journal of applied microbiology.

[52]  M. Wisniewski,et al.  Characterization of extracellular lytic enzymes produced by the yeast biocontrol agent Candida oleophila , 2004, Current Genetics.

[53]  B. Glick,et al.  Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. , 2003, Physiologia plantarum.

[54]  M. Silva-Filho,et al.  Soybean leghemoglobin targeted to potato chloroplasts influences growth and development of transgenic plants , 2000, Plant Cell Reports.

[55]  H. Fukuhara,et al.  Secreted Phytase Activities of Yeasts , 2000, Bioscience, biotechnology, and biochemistry.

[56]  T. Boller,et al.  Colorimetric assay for chitinase , 1988 .

[57]  D. Eveleigh,et al.  Cellulase screening by iodine staining: An artefact , 1987 .

[58]  J. Neilands,et al.  Universal chemical assay for the detection and determination of siderophores. , 1987, Analytical biochemistry.

[59]  H. Greppin,et al.  Peroxidase Release Induced by Ozone in Sedum album Leaves: Involvement of Ca. , 1984, Plant physiology.

[60]  J. Lockwood,et al.  Powdered Chitin Agar as a Selective Medium for Enumeration of Actinomycetes in Water and Soil , 1975 .

[61]  A. Rovira,et al.  MICROBIOLOGICAL STUDIES OF SOME SUBANTARCTIC SOILS , 1955 .

[62]  S. A. Gordon,et al.  COLORIMETRIC ESTIMATION OF INDOLEACETIC ACID. , 1951, Plant physiology.

[63]  L. J. Wickerham,et al.  Carbon Assimilation Tests for the Classification of Yeasts , 1948, Journal of bacteriology.

[64]  J. S. Simmons.  A Culture Medium for Differentiating Organisms of Typhoid-Colon Aerogenes Groups and for Isolation of Certain Fungi With Colored Plate , 1926 .