Introgression of durable blast resistance gene Pi-54 into indica rice cv. samba mahsuri, through Marker Assisted Backcross Breeding

Blast disease is one of the most significant diseases of rice, where severe infection results in more than 80% reduction in yield. It is caused by Pyricularia oryzae. New breeding strategies are essential for developing durable blast resistant varieties. The present study aimed to introgress Pi-54 gene from highly blast resistant genotype i.e. Tetep into elite rice cultivar Samba Mahsuri (BPT 5204), high yielding rice variety with good cooking quality ,but susceptible to blast disease, through Marker-Assisted Backcross Breeding programme (MABB). For foreground selection tightly linked molecular marker specific to Pi-54 gene (i.e. Pi-54MAS) which is located on chromosome 11, and it is utilized at each backcrossed generation to identify plants carrying heterozygous alleles for the targeted resistant gene. A total of 56 background markers were used to estimate the recovery of recurrent parent genome in each backcrossed generations. At BC2F2, a single plant carrying the targeted resistant gene Pi-54 with maximum recovery of recurrent parent genome (~92.80%; plant BT-8-47-22) was selected and forwarded to next generations through the selfing. Ancestry based selection procedure was employed for phenotypic disease screening and agro-morphological traits. Results confirmed that, resistance gene (Pi-54) was successfully incorporated into Samba Mahsuri. Six lines viz., BT-8-47-22-6-36, BT-8-47-22-6-55, BT-8-47-22-6-117, BT-8-47-22-6-159, BT-8-47-22-6-203 and BT-8-47-22-6-267 were identified at BC2F4 which were possessing high level of resistance to blast and agro-morphological traits similar to Samba Mahsuri. One NIL line (BT-8-47-22-6-203) was found to be better than recurrent parent Samba Mahsuri (BPT 5204) regarding grain yield per plant. This finding will be helpful in developing a blast resistant variety with highest recurrent parent genome recovery in less number of generations through the application of MABB.

[1]  R. Rani,et al.  Integrating marker-assisted background analysis with foreground selection for pyramiding bacterial blight resistance genes into Basmati rice. , 2018, Comptes rendus biologies.

[2]  Yuri Kotliarov,et al.  Assessment of Variability in the SOMAscan Assay , 2017, Scientific Reports.

[3]  T. Mou,et al.  Molecular breeding of thermo-sensitive genic male sterile (TGMS) lines of rice for blast resistance using Pi2 gene , 2015, Rice.

[4]  M. Rafii,et al.  Introgression of Pi-kh resistance gene into a Malaysian cultivar, MR264 using Marker-Assisted Backcrossing (MABC). , 2015 .

[5]  M. Seshumadhav.  Marker assisted introgression of BB and blast resistant genes in mega rice variety MTU1010 , 2014 .

[6]  R. K. Reddy,et al.  MARKER ASSISTED SELECTION IN DISEASE RESISTANCE BREEDING , 2013 .

[7]  M. Latif,et al.  Blast resistance in rice: a review of conventional breeding to molecular approaches , 2013, Molecular Biology Reports.

[8]  M. Alam,et al.  Marker-assisted Foreground Selection for Identification of Salt Tolerant Rice Genotypes , 2012 .

[9]  Ashutosh Kumar Singh,et al.  Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line, through marker assisted backcross breeding , 2012 .

[10]  Bijun Gao,et al.  Introgressing Blast Resistant Gene Pi-9(t) into Elite Rice Restorer Luhui17 by Marker-Assisted Selection , 2012 .

[11]  B. C. Viraktamath,et al.  Introgression of Blast Resistance Gene Pi-kh into Elite indica Rice Variety Improved Samba Mahsuri , 2012 .

[12]  B. C. Viraktamath,et al.  Marker‐assisted improvement of a stable restorer line, KMR‐3R and its derived hybrid KRH2 for bacterial blight resistance and grain quality , 2011 .

[13]  Ashutosh Kumar Singh,et al.  Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid , 2010, Molecular Breeding.

[14]  D. Mackill.  Breeding for Resistance to Abiotic Stresses in Rice: The Value of Quantitative Trait Loci , 2008 .

[15]  D. Mackill,et al.  Marker-assisted selection: an approach for precision plant breeding in the twenty-first century , 2008, Philosophical Transactions of the Royal Society B: Biological Sciences.

[16]  Han Qing-dian.  The screening of molecular markers closely linked to rice blast resistant gene Pi-1 and their application , 2005 .

[17]  N. Baisakh,et al.  Molecular Breeding for the Development of Blast and Bacterial Blight Resistance in Rice cv. IR50 , 2002 .

[18]  G. Lu,et al.  Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6 , 2002, Molecular Genetics and Genomics.

[19]  F. Hospital.  Size of donor chromosome segments around introgressed loci and reduction of linkage drag in marker-assisted backcross programs. , 2001, Genetics.

[20]  R. Berloo.  GGT: Software for the display of graphical genotypes , 1999 .

[21]  D. Somers,et al.  Molecular markers linked to white rust resistance in mustard Brassica juncea , 1998, Theoretical and Applied Genetics.

[22]  L. R. Pollard,et al.  Multiple disease and insect resistance for increased yield stability in rice. , 1989 .

[23]  K. Frey,et al.  Yield-component Analysis of Oat Isolines that Produce Different Grain Yields 1 , 1977 .