Polybrominated diphenyl ethers (PBDEs) were examined in fish fillets collected from two large Virginia watersheds. Emphasis was on the tetra- to hexabrominated congeners since these exhibit the greatest bioaccumulation and toxicological potentials. These congeners are dominant constituents of Penta-, a commercial PBDE product used to flame retard polyurethane foam. In 1999, North America accounted for98% of global Penta-demand. Concentrations of total tetra- to hexabrominated congeners in fillets ranged from <5 to 47,900 microg/kg (lipid basis). BDE-47, one of the two major constituents of Penta-, was detected in 89% of samples and contributed 40-70% of the total PBDEs observed. Concentrations of BDE-99, the second major constituent of the Penta- commercial mixture, were much lower in fish. While some differences in PBDE profiles between fish species were apparent, dominant congeners were consistent with those in surficial sediments from the Virginia sites and recently published data for U.S. air samples. PCB and PBDE concentrations in fish were generally associated. These factors point to exposure from nonpoint sources. Exceptions existed, likely due to inputs from local sources. The Virginia study area has historically served as a center for furniture and textile manufacturing, although polyurethane foam production here has been limited.