The Influence of Temperature on Metabolisms of Phosphorus Accumulating Organisms in Biological Wastewater Treatment Plants in the Presence of Cu(II) Toxicity

The purpose of this study was to study how temperature variation affects the tolerance of phosphorus accumulating organisms (PAOs) in a toxic environment. To exclude the interference of glycogen accumulating organisms (GAOs), shock loading experiments were conducted to study the effect of Cu(II) toxicity on the metabolisms of PAOs in 10, 20, and 30 ◦C conditions. The experimental data showed that the temperature effects on aerobic phosphorus uptake, PHA degradation, and glycogen synthesis were remarkable in the presence of Cu(II). Nevertheless, insignificant effects on anaerobic phosphorus release and PHA synthesis were found. The largest inhibition of PAO metabolism occurred in the low temperature case (10 ◦C). This study also experimentally demonstrated the loss of PAO metabolic ability in the subsequent aerobic stage, after the anaerobic stage. The presence of Cu(II) toxicity mainly resulted from the inhibition of biochemical reactions in the aerobic stage, and it was irrelevant to the inhibition of previous anaerobic metabolisms.

[1]  Jian Yang,et al.  Effect of copper ion on the anaerobic and aerobic metabolism of phosphorus-accumulating organisms linked to intracellular storage compounds. , 2011, Journal of hazardous materials.

[2]  A. Dobson,et al.  Parameters affecting biological phosphate removal from wastewaters. , 2004, Environment international.

[3]  J. Sibony,et al.  Biological Phosphorus Removal in France , 1987 .

[4]  T. Pai,et al.  Effect of cadmium on composition and diversity of bacterial communities in activated sludges , 2005 .

[5]  Jae-Kwang Park,et al.  Competition between Polyphosphate‐ and Glycogen‐Accumulating Organisms in Enhanced‐Biological‐Phosphorus‐Removal Systems: Effect of Temperature and Sludge Age , 2006, Water environment research : a research publication of the Water Environment Federation.

[6]  J. Neethling,et al.  Factors Influencing the Reliability of Enhanced Biological Phosphorus Removal , 2015 .

[7]  S. You,et al.  Effect of heavy metals on nitrification performance in different activated sludge processes. , 2009, Journal of hazardous materials.

[8]  J. Heijnen,et al.  Microbiology and biochemistry of the enhanced biological phosphate removal process , 1998 .

[9]  J J Heijnen,et al.  Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence , 1994, Biotechnology and bioengineering.

[10]  A. Converti,et al.  Biological removal of phosphorus from wastewaters by alternating aerobic and anaerobic conditions , 1995 .

[11]  N. Ren,et al.  Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system , 2011 .

[12]  W. Wu,et al.  Estimating Biomass of Heterotrophic and Autotrophic Bacteria by our Batch Tests , 2005, Environmental technology.

[13]  Seyoum Y. Gebremariam,et al.  Research Advances and Challenges in the Microbiology of Enhanced Biological Phosphorus Removal—A Critical Review , 2011, Water environment research : a research publication of the Water Environment Federation.

[14]  Damir Brdjanovic,et al.  TEMPERATURE EFFECTS ON PHYSIOLOGY OF BIOLOGICAL PHOSPHORUS REMOVAL , 1997 .

[15]  J. Anotai,et al.  Temperature effect on microbial community of enhanced biological phosphorus removal system. , 2003, Water research.

[16]  Zhiguo Yuan,et al.  Advances in enhanced biological phosphorus removal: from micro to macro scale. , 2007, Water research.

[17]  T. Pai,et al.  Effect of Cd(II) on different bacterial species present in a single sludge activated sludge process for carbon and nutrient removal , 2006 .

[18]  D. Jenkins,et al.  The Effects of MCRT and Temperature on Enhanced Biological Phosphorus Removal , 1992 .

[19]  C. W. Randall,et al.  Effects of temperature and mean cell residence time on biological nutrient removal processes , 1993 .

[20]  S. You,et al.  Metabolic influence of lead on polyhydroxyalkanoates (PHA) production and phosphate uptake in activated sludge fed with glucose or acetic acid as carbon source. , 2011, Bioresource technology.

[21]  R. Speece,et al.  Elutriated acid fermentation of municipal primary sludge. , 2006, Water research.

[22]  S. Rayne,et al.  Evidence for Tin Inhibition of Enhanced Biological Phosphorus Removal at a Municipal Wastewater Treatment Plant , 2005, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[23]  C. M. Hooijmans,et al.  Modeling the PAO-GAO competition: effects of carbon source, pH and temperature. , 2009, Water research.

[24]  Y. Tsai,et al.  Influence of sludge retention time on tolerance of copper toxicity for polyphosphate accumulating organisms linked to polyhydroxyalkanoates metabolism and phosphate removal. , 2011, Bioresource technology.

[25]  S. You,et al.  Effects of Heavy Metals on the Specific Ammonia and Nitrate Uptake Rates in Activated Sludge , 2009 .

[26]  Jan-Wei Lin,et al.  Verification of enzymes deterioration due to Cu(II) presence in an enhanced biological phosphorus removal system. , 2013, Chemosphere.

[27]  H. Santos,et al.  Model for carbon metabolism in biological phosphorus removal processes based on in vivo13C-NMR labelling experiments , 1996 .