Microvessel density and vascular endothelial growth factor expression in sinus augmentation using Bio-Oss.

AIM The aim of this study was to evaluate microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression in sinus augmentation with Bio-Oss. METHODS Twenty patients participated in this study. The sinuses were filled with 100% Bio-Oss. Implants were inserted after 3 months in group A, and 6 months in group B. A trephine was used to harvest bone cores. As control, the pre-existing subantral bone was used. RESULTS The mean MVD in control bone was 23.6 +/- 1.8. In the sites augmented with Bio-Oss, at 3 months, the MVD was 23.3 +/- 2.1, while in the sites retrieved at 6 months the MVD was 29.5 +/- 2.4. The difference in MVD between the control bone and group A was not statistically significant. The difference between the control bone and group B was statistically significant (P < 0.05). The statistical analysis showed that the difference in MVD between group A and group B was statistically significant (P < 0.05). CONCLUSIONS Bio-Oss seemed to induce an increase in MVD that reached a higher value after 6 months. The percentage of vessels positive to VEGF was higher in group A than in group B. Our data also showed a higher percentage of vessel and stromal cells positive to VEGF and higher MVD values in areas where there was newly formed bone compared with areas where maturation processes were occuring, and this fact could point to a close spatial relationship between angiogenesis and osteogenesis.

[1]  Georg N Duda,et al.  Initial vascularization and tissue differentiation are influenced by fixation stability , 2005, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[2]  Z. Shang,et al.  Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in oral squamous cell carcinoma: its correlation with angiogenesis and disease progression. , 2005, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[3]  Carlos E Nemcovsky,et al.  The amount of newly formed bone in sinus grafting procedures depends on tissue depth as well as the type and residual amount of the grafted material. , 2005, Journal of clinical periodontology.

[4]  D. Mooney,et al.  Combined Angiogenic and Osteogenic Factor Delivery Enhances Bone Marrow Stromal Cell‐Driven Bone Regeneration , 2004, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[5]  M. Battino,et al.  Immunohistochemical analysis of soft tissues in implants with healthy and peri-implantitis condition, and aggressive periodontitis. , 2004, Clinical oral implants research.

[6]  M. Lafage-Proust,et al.  Increase of Both Angiogenesis and Bone Mass in Response to Exercise Depends on VEGF , 2004, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[7]  H. Nygren,et al.  Implantation of hydrophilic and hydrophobic titanium discs in rat tibia: cellular reactions on the surfaces during the first 3 weeks in bone. , 2004, Biomaterials.

[8]  E. Tanaka,et al.  Immunolocalization of vascular endothelial growth factor in rat condylar cartilage during postnatal development , 2004, Histochemistry and Cell Biology.

[9]  R. Carano,et al.  Angiogenesis and bone repair. , 2003, Drug discovery today.

[10]  C. Maiorana,et al.  Maxillary sinus augmentation with anorganic bovine bone (Bio-Oss) and autologous platelet-rich plasma: preliminary clinical and histologic evaluations. , 2003, The International journal of periodontics & restorative dentistry.

[11]  A. Icaro Cornaglia,et al.  Ten-year follow-up in a maxillary sinus augmentation using anorganic bovine bone (Bio-Oss). A case report with histomorphometric evaluation. , 2003, Clinical oral implants research.

[12]  Kazuyoshi Obayashi,et al.  Microvascular response in the periodontal ligament following mucoperiosteal flap surgery. , 2003, Journal of periodontology.

[13]  I. Hvid,et al.  Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF‐inhibitor to the rabbit tibia during distraction osteogenesis , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[14]  E. Burger,et al.  Deproteinized cancellous bovine bone (Bio-Oss) as bone substitute for sinus floor elevation. A retrospective, histomorphometrical study of five cases. , 2003, Journal of clinical periodontology.

[15]  D. Buchbinder,et al.  Maxillary sinus augmentation with deproteinated bovine bone and platelet rich plasma with simultaneous insertion of endosseous implants. , 2003, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[16]  M. Merkx,et al.  Assessment of the value of anorganic bone additives in sinus floor augmentation: a review of clinical reports. , 2003, International journal of oral and maxillofacial surgery.

[17]  D. Dayan,et al.  Bovine-HA spongiosa blocks and immediate implant placement in sinus augmentation procedures. Histopathological and histomorphometric observations on different histological stainings in 10 consecutive patients. , 2002, Clinical oral implants research.

[18]  R. Haas,et al.  Porous hydroxyapatite for grafting the maxillary sinus: a comparative histomorphometric study in sheep. , 2002, The International journal of oral & maxillofacial implants.

[19]  S. Lindskog,et al.  A clinical histologic study of bovine hydroxyapatite in combination with autogenous bone and fibrin glue for maxillary sinus floor augmentation. Results after 6 to 8 months of healing. , 2001, Clinical oral implants research.

[20]  D. Carnes,et al.  Ability of deproteinized cancellous bovine bone to induce new bone formation. , 2000, Journal of periodontology.

[21]  A. Scarano,et al.  Bone reactions to anorganic bovine bone (Bio-Oss) used in sinus augmentation procedures: a histologic long-term report of 20 cases in humans. , 1999, The International journal of oral & maxillofacial implants.

[22]  R. B. Johnson,et al.  Vascular endothelial growth factors and progression of periodontal diseases. , 1999, Journal of periodontology.

[23]  H Winet,et al.  The role of microvasculature in normal and perturbed bone healing as revealed by intravital microscopy. , 1996, Bone.

[24]  M. Matsuda,et al.  Angiogenesis after sintered bone implantation in rat parietal bone. , 2003, Histology and histopathology.

[25]  J. Folkman.  Angiogenesis in cancer, vascular, rheumatoid and other disease , 1995, Nature Medicine.