The protective effect of ozone oxidative preconditioning against hypoxia/reoxygenation injury in rat kidney cells

Abstract Ozone (O3) has been viewed as a novel treatment for different diseases in these years and oxidative stress and apoptosis play a key role in the pathogenesis of kidney diseases including renal ischemia and reperfusion (I/R). In the present study, we investigated the role of ozone oxidative preconditioning (OzoneOP) in attenuating oxidative stress and apoptosis in a hypoxia/reoxygenation (H/R) injury model using rat kidney cells. We induced H/R injury in kidney cells treated with or without OzoneOP. Oxidative stress parameters such as superoxide dismutase (SOD), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were determined, as well as some apoptotic proteins. We observed that oxidative stress and apoptosis were increased in H/R group compared to OzoneOP group; however, these changes were significantly decreased by the treatment with OzoneOP. We concluded that OzoneOP can protect the kidney cells against H/R injury and its mechanism may be through the reduction of oxidative stress and apoptosis.

[1]  Haixin Hong,et al.  Neutrophil gelatinase-associated lipocalin protects renal tubular epithelial cells in hypoxia–reperfusion by reducing apoptosis , 2014, International Urology and Nephrology.

[2]  B. Yuan,et al.  Effect of ischemia post-conditioning on skeletal muscle oxidative injury, mTOR, Bax, Bcl-2 proteins expression, and HIF-1α/β-actin mRNA, IL-6/β-actin mRNA and caveolin-3/β-actin mRNA expression in ischemia–reperfusion rabbits , 2012, Molecular Biology Reports.

[3]  Bo Yu,et al.  The protective effect of picroside II against hypoxia/reoxygenation injury in neonatal rat cardiomyocytes , 2012, Pharmaceutical biology.

[4]  A. Attia,et al.  Cardioprotective effects of ozone oxidative preconditioning in an in vivo model of ischemia/reperfusion injury in rats , 2012, Scandinavian journal of clinical and laboratory investigation.

[5]  E. Çakır,et al.  The Effects of Medical Ozone Therapy on Renal Ischemia/Reperfusion Injury , 2012, Renal failure.

[6]  A. Lau,et al.  Osteopontin Expressed in Tubular Epithelial Cells Regulates NK Cell-Mediated Kidney Ischemia Reperfusion Injury , 2010, The Journal of Immunology.

[7]  C. Wanner,et al.  Implementation of an in vitro Model System for Investigation of Reperfusion Damage after Renal Ischemia , 2009, Cellular Physiology and Biochemistry.

[8]  P. Chen,et al.  Ischemic postconditioning modified renal oxidative stress and lipid peroxidation caused by ischemic reperfusion injury in rats. , 2009, Transplantation proceedings.

[9]  G. Klintmalm,et al.  Acute kidney injury following liver transplantation: Definition and outcome , 2009, Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society.

[10]  T. Sun,et al.  Effects of different concentrations of oxygen – ozone on rats’ astrocytes in vitro , 2008, Neuroscience Letters.

[11]  Hui Chen,et al.  Ozone oxidative preconditioning inhibits inflammation and apoptosis in a rat model of renal ischemia/reperfusion injury. , 2008, European journal of pharmacology.

[12]  Alissa L. Russ,et al.  Experimental strategies to improve in vitro models of renal ischemia. , 2007, Experimental and molecular pathology.

[13]  E. Candelario-Jalil,et al.  Therapeutic efficacy of ozone in patients with diabetic foot. , 2005, European journal of pharmacology.

[14]  R. Ferrari,et al.  The occurrence of oxidative stress during reperfusion in experimental animals and men , 1991, Cardiovascular Drugs and Therapy.

[15]  G. Murray,et al.  Phosphocreatine restores high-energy phosphates in ischemic myocardium: implication for off-pump cardiac revascularization. , 2003, Journal of the American College of Surgeons.

[16]  R. Bartrons,et al.  Effect of ozone treatment on reactive oxygen species and adenosine production during hepatic ischemia-reperfusion , 2000, Free radical research.

[17]  G. Valacchi,et al.  Studies on the biological effects of ozone: 11. Release of factors from human endothelial cells. , 2000, Mediators of inflammation.

[18]  J. Roselló-Catafau,et al.  Protective effect of ozone treatment on the injury associated with hepatic ischemia-reperfusion: antioxidant-prooxidant balance. , 1999, Free radical research.

[19]  V Bocci,et al.  Biological and clinical effects of ozone. Has ozone therapy a future in medicine? , 1999, British journal of biomedical science.

[20]  R. Castillo,et al.  Ozone oxidative preconditioning: a protection against cellular damage by free radicals. , 1998, Mediators of Inflammation.

[21]  W. Pryor.  Mechanisms of radical formation from reactions of ozone with target molecules in the lung. , 1994, Free radical biology & medicine.