15N NMR spectroscopy 31†—preliminary study on the insulin-a chain (bunte salt form)

The Bunte salt derivative was prepared from bovine insulin-A isolated from natural sources, and was purified by chromatography. Natural abundance 40.55 MHz 15N NMR spectra were measured in water and dimethyl sulphoxide. The latter solvent gave the best resolution, and 20 of the 22 possible amide nitrogen signals were detected. Several model oligopeptides were also measured and their usefulness for assignments is discussed.

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