Combined effect of grain refinement and surface modification of pure titanium on the attachment of mesenchymal stem cells and osteoblast-like SaOS-2 cells.

Surface modification is an important step in production of medical implants. Surface roughening creates additional surface area to enhance the bonding between the implant and the bone. Recent research provided a means to alter the microstructure of titanium by severe plastic deformation (SPD) in order to increase its strength, and thereby reduce the size of the implants (specifically, their diameter). The purpose of the present study was to examine the effect of bulk microstructure of commercially pure titanium with coarse-grained (CG) and ultrafine-grained (UFG) bulk structure on the surface state of these materials after surface modification by sand blasting and acid etching (SLA). It was shown that SLA-modified surface characteristics, in particular, roughness, chemistry, and wettability, were affected by prior SPD processing. Additionally, biocompatibility of UFG titanium was examined using osteosarcoma cell line SaOS-2 and primary human adipose-derived mesenchymal stem cell (adMSC) cultures. Enhanced cell viability as well as increased matrix mineralization during osteogenic differentiation of MSCs on the surface of ultrafine-grained titanium was shown.

[1]  Maryam Tabrizian,et al.  The significance of crystallographic texture of titanium alloy substrates on pre-osteoblast responses. , 2006, Biomaterials.

[2]  Yong Wang,et al.  Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(L-lactide). , 2005, Biomaterials.

[3]  I. Beyerlein,et al.  Texture evolution in equal-channel angular extrusion , 2009 .

[4]  Hyeran Noh,et al.  Surface energy effects on osteoblast spatial growth and mineralization. , 2008, Biomaterials.

[5]  Abraham Marmur,et al.  A review on the wettability of dental implant surfaces I: theoretical and experimental aspects. , 2014, Acta biomaterialia.

[6]  F Rupp,et al.  High surface energy enhances cell response to titanium substrate microstructure. , 2005, Journal of biomedical materials research. Part A.

[7]  Sriram Natarajan,et al.  Review: Micro- and nanostructured surface engineering for biomedical applications , 2013 .

[8]  Thomas J Webster,et al.  Increased osteoblast adhesion on nanophase metals: Ti, Ti6Al4V, and CoCrMo. , 2004, Biomaterials.

[9]  Y. Estrin,et al.  Accelerated stem cell attachment to ultrafine grained titanium. , 2011, Acta biomaterialia.

[10]  Y. Estrin,et al.  Fatigue behaviour of light alloys with ultrafine grain structure produced by severe plastic deformation: An overview , 2010 .

[11]  Richard O. Hynes,et al.  Integrins: Versatility, modulation, and signaling in cell adhesion , 1992, Cell.

[12]  Didem Öner,et al.  Ultrahydrophobic Surfaces. Effects of Topography Length Scales on Wettability , 2000 .

[13]  Wei Liu,et al.  Calculation of the surface energy of bcc-metals with the empirical electron theory , 2009 .

[14]  V. V. Latysh,et al.  Nanostructured Titanium for Biomedical Applications , 2008 .

[15]  T. Webster,et al.  The effect of nanotopography on calcium and phosphorus deposition on metallic materials in vitro. , 2006, Biomaterials.

[16]  S. Bondy.  The neurotoxicity of environmental aluminum is still an issue. , 2010, Neurotoxicology.

[17]  Bum-Su Kim,et al.  An analysis of failure of 5-year loaded tapered implants with SLA surface , 2014 .

[18]  C. Wen,et al.  The influence of surface energy of titanium-zirconium alloy on osteoblast cell functions in vitro. , 2011, Journal of biomedical materials research. Part A.

[19]  T. Albrektsson,et al.  Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations, surface roughness, crystal structure and chemical composition. , 2002, Biomaterials.

[20]  David F. Williams.  On the mechanisms of biocompatibility. , 2008, Biomaterials.

[21]  L. Gao,et al.  Hydrophilicity of TiO2 films prepared by liquid phase deposition , 2000 .

[22]  N. Orhan,et al.  Interface characterisation of diffusion bonded Ti–6Al–4V alloy and austenitic stainless steel couple , 2009 .

[23]  Maxence Bigerelle,et al.  The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour. , 2000, Biomaterials.

[24]  A. Iregren,et al.  Chapter 26 – Aluminum , 2015 .

[25]  D. L. Cochran,et al.  Osteoblast-Mediated Mineral Deposition in Culture is Dependent on Surface Microtopography , 2002, Calcified Tissue International.

[26]  M. van Griensven,et al.  Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue. , 2007, Tissue engineering.

[27]  T. Taylor,et al.  A 5-year prospective multicenter study of early loaded titanium implants with a sandblasted and acid-etched surface. , 2011, The International journal of oral & maxillofacial implants.

[28]  P. Layrolle,et al.  Surface treatments of titanium dental implants for rapid osseointegration. , 2007, Dental materials : official publication of the Academy of Dental Materials.

[29]  J. Bernard,et al.  Biological properties of acid etched titanium implants: effect of sandblasting on bone anchorage. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[30]  O. Akhavan,et al.  Self-accumulated Ag nanoparticles on mesoporous TiO2 thin film with high bactericidal activities , 2010 .

[31]  C. Wilkinson,et al.  Osteoprogenitor response to defined topographies with nanoscale depths. , 2006, Biomaterials.

[32]  W. Att,et al.  The effect of superficial chemistry of titanium on osteoblastic function. , 2008, Journal of biomedical materials research. Part A.

[33]  W. Soboyejo,et al.  Cell/surface interactions and adhesion on Ti-6Al-4V: effects of surface texture. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[34]  T. Albrektsson,et al.  Effects of titanium surface topography on bone integration: a systematic review. , 2009, Clinical oral implants research.

[35]  C. Wilkinson,et al.  The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. , 2007, Nature materials.

[36]  Anne E Meyer,et al.  Effect of cleaning and sterilization on titanium implant surface properties and cellular response. , 2012, Acta biomaterialia.

[37]  S. O. Azi,et al.  Surface energies of hcp metals using equivalent crystal theory , 2009 .

[38]  J. Pan,et al.  Electrochemical impedance spectroscopy study of the passive oxide film on titanium for implant application , 1996 .

[39]  Tomas Albrektsson,et al.  The bone response of oxidized bioactive and non-bioactive titanium implants. , 2005, Biomaterials.

[40]  T. Webster,et al.  Using mathematical models to understand the effect of nanoscale roughness on protein adsorption for improving medical devices , 2013, International journal of nanomedicine.

[41]  Y. Estrin,et al.  Extreme grain refinement by severe plastic deformation: A wealth of challenging science , 2013 .

[42]  Vaclav Svorcik,et al.  Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. , 2011, Biotechnology advances.

[43]  C J Murphy,et al.  Effects of synthetic micro- and nano-structured surfaces on cell behavior. , 1999, Biomaterials.

[44]  B. Nebe,et al.  Osteoblast Sensitivity to Topographical and Chemical Features of Titanium , 2010 .

[45]  R. Lapovok,et al.  Accelerated growth of preosteoblastic cells on ultrafine grained titanium. , 2009, Journal of biomedical materials research. Part A.

[46]  Lourdes Díaz-Rodríguez,et al.  Effect of roughness, wettability and morphology of engineered titanium surfaces on osteoblast-like cell adhesion , 2010 .

[47]  C. Ramseier,et al.  10-year survival and success rates of 511 titanium implants with a sandblasted and acid-etched surface: a retrospective study in 303 partially edentulous patients. , 2012, Clinical implant dentistry and related research.

[48]  J. Kollár,et al.  The surface energy of metals , 1998 .

[49]  H. Rack,et al.  Titanium alloys in total joint replacement--a materials science perspective. , 1998, Biomaterials.

[50]  B. B. Panigrahi,et al.  In vitro fibroblast response to ultra fine grained titanium produced by a severe plastic deformation process , 2008, Journal of materials science. Materials in medicine.

[51]  A. Mata,et al.  Fabrication of hierarchical micro–nanotopographies for cell attachment studies , 2013, Nanotechnology.

[52]  Doron Steinberg,et al.  Adsorption of human plasma proteins to modified titanium surfaces. , 2007, Clinical oral implants research.

[53]  Andrea R. Gerson,et al.  Resolving surface chemical states in XPS analysis of first row transition metals, oxides and hydroxides: Sc, Ti, V, Cu and Zn , 2010 .

[54]  M. Jäger,et al.  Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants , 2007, Journal of biomedicine & biotechnology.

[55]  K. Smetana,et al.  An in vivo Evaluation of Ultra-fine Grained Titanium Implants , 2009 .

[56]  In-Seop Lee,et al.  The biocompatibility of SLA-treated titanium implants , 2008, Biomedical materials.

[57]  N. Donos,et al.  Modified titanium surfaces promote accelerated osteogenic differentiation of mesenchymal stromal cells in vitro. , 2009, Bone.

[58]  H. Sakurai,et al.  Vanadium distribution in rats and DNA cleavage by vanadyl complex: implication for vanadium toxicity and biological effects. , 1994, Environmental health perspectives.

[59]  P. Layrolle,et al.  Osteoblastic cell behaviour on different titanium implant surfaces. , 2008, Acta biomaterialia.

[60]  B. Delmon,et al.  Influence of the amount of titania on the texture and structure of titania supported on silica , 1996 .

[61]  W. Lukiw,et al.  Towards the prevention of potential aluminum toxic effects and an effective treatment for Alzheimer's disease. , 2011, Journal of inorganic biochemistry.

[62]  Marion Frant,et al.  The effect of positively charged plasma polymerization on initial osteoblastic focal adhesion on titanium surfaces. , 2007, Biomaterials.

[63]  Robert M. Wallace,et al.  SiO2 film thickness metrology by x-ray photoelectron spectroscopy , 1997 .

[64]  Mark C. Biesinger,et al.  X-ray photoelectron spectroscopy studies of reactions on chromium metal and chromium oxide surfaces , 2011 .

[65]  T. Webster,et al.  Increased functions of osteoblasts on nanophase metals , 2007 .

[66]  D. Moratal,et al.  Effect of nanoscale topography on fibronectin adsorption, focal adhesion size and matrix organisation. , 2010, Colloids and surfaces. B, Biointerfaces.

[67]  Marcus Textor,et al.  Titanium in Medicine : material science, surface science, engineering, biological responses and medical applications , 2001 .

[68]  Y. Estrin,et al.  Comparison of laboratory-scale and industrial-scale equal channel angular pressing of commercial purity titanium , 2015 .

[69]  A. Oskarsson,et al.  Chapter 60 – Vanadium , 2015 .

[70]  H. Matusiewicz.  Potential release of in vivo trace metals from metallic medical implants in the human body: from ions to nanoparticles--a systematic analytical review. , 2014, Acta biomaterialia.

[71]  Yufeng Zheng,et al.  In vitro and in vivo evaluation of SLA titanium surfaces with further alkali or hydrogen peroxide and heat treatment , 2011, Biomedical materials.

[72]  Carlos Nelson Elias,et al.  Relationship between surface properties (roughness, wettability and morphology) of titanium and dental implant removal torque. , 2008, Journal of the mechanical behavior of biomedical materials.

[73]  Jan E Ellingsen,et al.  Titanium implant surface modification by cathodic reduction in hydrofluoric acid: surface characterization and in vivo performance. , 2009, Journal of biomedical materials research. Part A.

[74]  M. Dettin,et al.  Novel immobilizations of an adhesion peptide on the TiO2 surface: An XPS investigation , 2007 .

[75]  Yasuhiro Tanimoto,et al.  A review of improved fixation methods for dental implants. Part I: Surface optimization for rapid osseointegration. , 2015, Journal of prosthodontic research.

[76]  A. Singh,et al.  Ti based biomaterials, the ultimate choice for orthopaedic implants – A review , 2009 .

[77]  Newell R Washburn,et al.  Combinatorial screen of the effect of surface energy on fibronectin-mediated osteoblast adhesion, spreading and proliferation. , 2006, Biomaterials.

[78]  A. Medvedev,et al.  Effect of bulk microstructure of commercially pure titanium on surface characteristics and fatigue properties after surface modification by sand blasting and acid-etching. , 2016, Journal of the mechanical behavior of biomedical materials.

[79]  Megan S. Lord,et al.  Influence of nanoscale surface topography on protein adsorption and cellular response , 2010 .

[80]  T. Webster,et al.  Nanometer surface roughness increases select osteoblast adhesion on carbon nanofiber compacts. , 2004, Journal of biomedical materials research. Part A.

[81]  M. Cronin,et al.  Metals, toxicity and oxidative stress. , 2005, Current medicinal chemistry.

[82]  L. Schmidt,et al.  Sulfur on noble metal catalyst particles , 1982 .

[83]  T. Lee,et al.  Effects of nano-surface properties on initial osteoblast adhesion and Ca/P adsorption ability for titanium alloys , 2008, Nanotechnology.

[84]  Maxence Bigerelle,et al.  Wettability versus roughness: Multi-scales approach , 2015 .

[85]  JUAN JOSÉ RODRÍGUEZ MERCADO,et al.  Genotoxic effects of vanadium(IV) in human peripheral blood cells. , 2003, Toxicology letters.