Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates

Cell morphology, protein/DNA ratio, ganglioside analysis, taurine uptake, and the activity of neurone specific enolase showed that neuronal cells mature faster when grown on polyethyleneimine coated plates compared to cells grown on polylysine coated plates. Our results also show higher protein/DNA ratio and total and neuron specific enolase activities in cells grown in serum supplemented medium, when compared to their counterparts grown in synthetic medium. Moreover, our results show that only some specific markers can be used to determine the early and late events of cell maturation, whereas other markers continuously vary with time. © 1992 Wiley‐Liss, Inc.

[1]  E. Yavin,et al.  ATTACHMENT AND CULTURE OF DISSOCIATED CELLS FROM RAT EMBRYO CEREBRAL HEMISPHERES ON POLYLYSINE-COATED SURFACE , 1974, The Journal of cell biology.

[2]  M. Cousin,et al.  Detection and localization of 14-3-2 protein in primary cultures of embryonic rat brain , 1980, Brain Research.

[3]  Y. Courtois,et al.  Specific binding of basic fibroblast growth factor to basement membrane‐like structures and to purified heparan sulfate proteoglycan of the EHS tumor , 1988, Journal of cellular physiology.

[4]  L. Irwin,et al.  Ganglioside Patterns of Fetal Rat and Mouse Brain , 1980, Journal of neurochemistry.

[5]  G. G. Járos,et al.  Neuronal cells from chick embryo cerebral hemispheres cultivated on polylysine-coated surfaces. , 1978, Developmental neuroscience.

[6]  A. Lander,et al.  Purification of a factor that promotes neurite outgrowth: isolation of laminin and associated molecules , 1985, The Journal of cell biology.

[7]  W. Seifert,et al.  In-vitro and in-vivo studies on gangliosides in the developing and regenerating hippocampus of the rat. , 1984, Advances in experimental medicine and biology.

[8]  H. Kleinman,et al.  Use of extracellular matrix components for cell culture. , 1987, Analytical biochemistry.

[9]  D. Rifkin,et al.  Role of extracellular matrix in the action of basic fibroblast growth factor: Matrix as a source of growth factor for long‐term stimulation of plasminogen activator production and DNA synthesis , 1989, Journal of cellular physiology.

[10]  K. Kuriyama,et al.  Characteristics of taurine transport system and its developmental pattern in mouse cerebral cortical neurons in primary culture. , 1988, Biochimica et biophysica acta.

[11]  B. Pettmann,et al.  Morphological and biochemical maturation of neurones cultured in the absence of glial cells , 1979, Nature.

[12]  K. Paigen,et al.  A simple, rapid, and sensitive DNA assay procedure. , 1980, Analytical biochemistry.

[13]  M. Rusnati,et al.  Basic fibroblast growth factor is released from endothelial extracellular matrix in a biologically active form , 1989, Journal of cellular physiology.

[14]  P. Mandel,et al.  Neuronal and glial cell cultures, a tool for investigation of ganglioside function. , 1980, Advances in experimental medicine and biology.

[15]  P. Mandel,et al.  Gangliosides of glial cells: A comparative study of normal astroblasts in tissue culture and glial cells isolated on sucrose‐ficoll gradients , 1975, FEBS letters.

[16]  G. Rebel,et al.  Effect of hepes on the taurine uptake by cultured glial cells , 1989, Journal of neuroscience research.

[17]  F. Hefti,et al.  Growth of dissociated neurons in culture dishes coated with synthetic polymeric amines , 1984, Neuroscience Letters.

[18]  J. Louis,et al.  Long-term culture of neurones from human cerebral cortex in serum-free medium , 1983, Neuroscience Letters.

[19]  D. Reis,et al.  Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[20]  P. Mandel,et al.  Gangliosides in cultured neurons , 1980, Neuroscience.

[21]  K. Schilling,et al.  Expression of synaptophysin and neuron‐specific enolase during neuronal differentiation in vitro: Effects of dimethyl sulfoxide , 1989, Journal of neuroscience research.

[22]  K. Suzuki.  A SIMPLE AND ACCURATE MICROMETHOD FOR QUANTITATIVE DETERMINATION OF GANGLIOSIDE PATTERNS. , 1964, Life sciences.

[23]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[24]  M. Forbes,et al.  Phenotypic properties of catecholamine-positive cells that differentiate in avian neural crest cultures , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[25]  S. Avrameas,et al.  Modulation of cell behavior in vitro by the substratum in fibroblastic and leukemic mouse cell lines. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[26]  R. Adler.  Taurine uptake by chick embryo retinal neurons and glial cells in purified culture , 1983, Journal of neuroscience research.

[27]  G. Wilding,et al.  DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water. , 1990, Analytical biochemistry.

[28]  P. Mandel,et al.  Gangliosides of active and inactive neuroblastoma clones. , 1977, Differentiation; research in biological diversity.

[29]  J. Weyhenmeyer,et al.  Expression of neuron-specific enolase in cultured neurons from the fetal rat , 1983, Neuroscience Letters.

[30]  J. Goldman,et al.  GD3 ganglioside is a glycolipid characteristic of immature neuroectodermal cells , 1985, Journal of Neuroimmunology.

[31]  J. Wood,et al.  Ganglioside patterns and cholera toxin--peroxidase labeling of aggregating cells from the chick optic tectum. , 1979, Journal of neurobiology.

[32]  T. Chiba,et al.  PLASTICITY OF PHEOCHROMOCYTOMA (PC12) CELLS DEMONSTRATED BY NERVE GROWTH FACTOR OR GLUCOCORTICOID TREATMENT: A CATECHOLAMINE FLUORESCENCE AND ELECTRON MICROSCOPIC INVESTIGATION , 1981 .

[33]  F. Šmíd,et al.  A densitometric method for the determination of gangliosides after their separation by thin-layer chromatography and detection with resorcinol reagent. , 1973, Journal of chromatography.

[34]  R. Adler,et al.  Neuritic guidance by polyornithine-attached materials of ganglionic origin. , 1981, Developmental biology.

[35]  J. Mester,et al.  Involvement of serum factor(s) adsorbed to the dish in the response of cycloheximide-pretreated BP-A31 cells to serum pulses. , 1988, Experimental cell research.

[36]  T. Miettinen,et al.  Use of Butyl Acetate in Determination of Sialic Acid. , 1959 .

[37]  C. Schengrund,et al.  Neuron‐specific enolase levels in primary cultures of neurons , 1980, Journal of neuroscience research.

[38]  P C Letourneau,et al.  Possible roles for cell-to-substratum adhesion in neuronal morphogenesis. , 1975, Developmental biology.

[39]  E. Yavin,et al.  Ganglioside Profiles during Neural Tissue Development , 1979 .

[40]  G H Sato,et al.  Growth of a rat neuroblastoma cell line in serum-free supplemented medium. , 1979, Proceedings of the National Academy of Sciences of the United States of America.