Analysis systems for the detection of ammonia based on micromachined components modular hybrid versus monolithic integrated approach

This paper presents two miniaturized analysis systems for measurement of the ammonia concentration in aqueous solutions. Both systems are based on identical micromachined components. In the first system the components are integrated on one chip, thus literally forming a "lab-on-a-chip". The second system uses the components as separate dies assembled in or on a printed circuit board (PCB) and connected via a micromachined fluidic channel plate. The latter modular system provides easy replacement of components, either to replace a malfunctioning component or to upgrade a component to an improved version. Additionally, replacement of modules enables a change in the functionality of the system in order to perform a different chemical analysis. With the monolithic and the modular system measurement of concentrations in the range 10-20 mM, respectively, 5.6-560 µM were demonstrated. With this paper, the authors intend to show that both the integrated as well as the modular approach of making a micromachined analytical system can result in working systems. Though the chemical behavior of both systems were not fully explored, it is made clear that both approaches can successfully be used for chemical analysis purposes.

[1]  Jan C.T. Eijkel,et al.  Micromachined heated chemical reactor for pre-column derivatisation , 1998 .

[2]  J Ruzicka,et al.  Micro sequential injection: fermentation monitoring of ammonia, glycerol, glucose, and free iron using the novel lab-on-valve system. , 2001, The Analyst.

[3]  J. G. Smits.  Piezoelectric micropump with three valves working peristaltically , 1990 .

[4]  D. J. Harrison,et al.  Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip , 1993, Science.

[5]  S. R. Crouch,et al.  Kinetic study of Berthelot reaction steps in the absence and presence of coupling reagents. , 1989, Talanta.

[6]  J. Michael Ramsey,et al.  Precolumn Reactions with Electrophoretic Analysis Integrated on a Microchip , 1994 .

[7]  M. A. Northrup,et al.  A miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers. , 1998, Analytical chemistry.

[8]  S. R. Crouch,et al.  Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia , 1977 .

[9]  P. L. Searle.  The berthelot or indophenol reaction and its use in the analytical chemistry of nitrogen. A review , 1984 .

[10]  Wolfgang Frenzel,et al.  Different approaches to the determination of ammonium ions at low levels by flow injection analysis , 1988 .

[11]  Albert van den Berg,et al.  Silicon microtechnology and microstructures in separation science , 1998 .

[12]  S. Terry,et al.  A gas chromatographic air analyzer fabricated on a silicon wafer , 1979, IEEE Transactions on Electron Devices.

[13]  Alan G. R. Evans,et al.  Two simple micromixers based on silicon , 1998 .

[14]  C. Strandman,et al.  Fabrication of 45/spl deg/ mirrors together with well-defined v-grooves using wet anisotropic etching of silicon , 1995 .

[15]  Norbert Schwesinger,et al.  A modular microfluid system with an integrated micromixer , 1996 .

[16]  Luke C. Adam,et al.  Hypochlorite Ion Decomposition: Effects of Temperature, Ionic Strength, and Chloride Ion. , 1999, Inorganic chemistry.

[17]  Louis Hellemans,et al.  Anisotropically Etched Silicon Mirrors for Optical Sensor Applications , 1995 .

[18]  T. T. Veenstra,et al.  Microfluidics and Microtechnology for Microreactor Systems , 2001 .

[19]  A. Berg,et al.  Micro Total Analysis Systems: Microfluidic Aspects, Integration Concept and Applications , 1997 .

[20]  Andreas Manz,et al.  Towards Integrated Continuous-Flow Chemical Reactors , 1999 .

[21]  M. A. Northrup,et al.  Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. , 1996, Analytical chemistry.

[22]  A. Manz,et al.  A silicon flow cell for optical detection in miniaturized total chemical analysis systems , 1992 .

[23]  Brian N. Johnson,et al.  An integrated nanoliter DNA analysis device. , 1998, Science.

[24]  J. Ruzicka.  Lab-on-valve: universal microflow analyzer based on sequential and bead injection , 2000 .

[25]  Peter De Dobbelaere,et al.  Micromachined Photodiode Submount with Integrated Mirror for Efficient Out-of-Plane Coupling to Planar Polymeric Waveguide Circuits , 1998 .

[26]  T. T. Veenstra.  MAFIAS: an integrated lab-on-a-chip for the measurement of ammonium , 2001 .

[27]  Albert van den Berg,et al.  Modular Setup for a Miniaturized Chemical Analysis System , 1993 .

[28]  J. Ruzicka,et al.  Flow injection analyses , 1975 .

[29]  Christopher J. Morris,et al.  Optimization of a circular piezoelectric bimorph for a micropump driver , 2000 .

[30]  W. E. van der Linden,et al.  Miniaturisation in flow injection analysis Practical limitations from a theoretical point of view , 1987 .

[31]  H. Lenhoff,et al.  Interference in determination of ammonia with the hypochlorite-alkaline phenol method of Berthelot , 1982 .

[32]  Miko Elwenspoek,et al.  Use of Selective Anodic Bonding to Create Micropump Chambers with Virtually No Dead Volume , 2001 .

[33]  A. Heuberger,et al.  Anisotropic Etching of Crystalline Silicon in Alkaline Solutions I . Orientation Dependence and Behavior of Passivation Layers , 1990 .

[34]  Jan H. J. Fluitman,et al.  Towards integrated microliquid handling systems , 1994 .

[35]  S. Jeanneret,et al.  Three-dimensional micro flow manifolds for miniaturized chemical analysis systems , 1994 .

[36]  D. J. Harrison,et al.  Capillary electrophoresis and sample injection systems integrated on a planar glass chip , 1992 .

[37]  Miko Elwenspoek,et al.  Characterization method for a new diffusion mixer applicable in micro flow injection analysis systems , 1999 .

[38]  J. McCaskill,et al.  Spatially resolved in vitro molecular ecology. , 1997, Biophysical chemistry.

[39]  T. T. Veenstra,et al.  A light detection cell to be used in a micro analysis system for ammonia. , 2002, Talanta.

[40]  A. Manz,et al.  Miniaturized total chemical analysis systems: A novel concept for chemical sensing , 1990 .

[41]  Dermot Diamond,et al.  Chemical sensing using an integrated microfluidic system based on the Berthelot reaction , 2001 .

[42]  Martin Richter,et al.  Robust design of gas and liquid micropumps , 1998 .