Expression analysis of sex-specific and 17beta-estradiol-responsive genes in the Japanese medaka, Oryzias latipes, using oligonucleotide microarrays.

Gene profiling of Japanese medaka (Oryzias latipes) was performed using an oligonucleotide DNA microarray representing 22,587 TIGR O. latipes gene indices (OLGIs). The average correlation coefficients for gene expression between individual mature fish were high (>0.95) for both female and male, indicating that the physiological status of medaka is highly reproducible under prescribed growth conditions. Of the 22,587 OLGIs, 2575 showed significant differences in expression between female and male. Exposure to 17beta-estradiol (E2) revealed 381 E2-responsive OLGIs in male medaka. Feminization and male-dysfunction factors of the E2-treated males calculated using the combination of Pearson correlation coefficient and Euclidean distances indicate that E2 treatment "weakly feminized" male medaka, while male physiological functions were not significantly disrupted. This study demonstrates the possibility of using medaka microarrays to estimate the overall effects of hormonally active chemicals.

[1]  J. Sumpter,et al.  Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment. , 1995, Environmental health perspectives.

[2]  G. Ramsay.  DNA chips: State-of-the art , 1998, Nature Biotechnology.

[3]  Y. Kohara,et al.  Large-scale isolation of ESTs from medaka embryos and its application to medaka developmental genetics , 2004, Mechanisms of Development.

[4]  C. E. Budde.  FIG , 2022, ACM SIGMETRICS Performance Evaluation Review.

[5]  J. Volff,et al.  Cloning of the dmrt1 gene of Xiphophorus maculatus: dmY/dmrt1Y is not the master sex-determining gene in the platyfish. , 2003, Gene.

[6]  A. Kanamori.  Systematic identification of genes expressed during early oogenesis in medaka , 2000, Molecular reproduction and development.

[7]  M Schena,et al.  Microarrays: biotechnology's discovery platform for functional genomics. , 1998, Trends in biotechnology.

[8]  K. Suzuki,et al.  Protamines , 1973, Molecular Biology Biochemistry and Biophysics.

[9]  Dianne Dumanoski,et al.  Our Stolen Future , 1996 .

[10]  Y. Wakamatsu,et al.  Medaka as a model of transgenic fish. , 1992, Molecular marine biology and biotechnology.

[11]  J. Eberwine,et al.  Analysis of gene expression in single live neurons. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[12]  T. Kondo,et al.  Introduction of cyclin B induces activation of the maturation-promoting factor and breakdown of germinal vesicle in growing zebrafish oocytes unresponsive to the maturation-inducing hormone. , 1997, Developmental biology.

[13]  I. Kang,et al.  Effect of 17β-estradiol on the reproduction of Japanese medaka (Oryzias latipes) , 2002 .

[14]  Y. Shimizu,et al.  Requirement of protamine for maintaining nuclear condensation of medaka (Oryzias latipes) spermatozoa shed into water but not for promoting nuclear condensation during spermatogenesis. , 2000, The International journal of developmental biology.

[15]  M. Sussman,et al.  Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array , 1999, Nature Biotechnology.

[16]  S. Soyal,et al.  FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation. , 2000, Development.

[17]  M. Matsuda.  Sex determination in fish: Lessons from the sex‐determining gene of the teleost medaka, Oryzias latipes , 2003, Development, growth & differentiation.

[18]  Y. Ishikawa,et al.  Medakafish as a model system for vertebrate developmental genetics. , 2000, BioEssays : news and reviews in molecular, cellular and developmental biology.

[19]  M. Schartl.  A comparative view on sex determination in medaka , 2004, Mechanisms of Development.

[20]  T. Speed,et al.  Summaries of Affymetrix GeneChip probe level data. , 2003, Nucleic acids research.

[21]  A. Arukwe,et al.  Molecular cloning of rainbow trout (Oncorhynchus mykiss) eggshell zona radiata protein complementary DNA: mRNA expression in 17beta-estradiol- and nonylphenol-treated fish. , 2002, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[22]  Tohru Kobayashi,et al.  Expression of DMY and DMTR1 in Various Tissues of the Medaka (Oryzias latipes) , 2003 .

[23]  S. Deschamps,et al.  Two forms of elongation factor 1 alpha (EF-1 alpha O and 42Sp50), present in oocytes, but absent in somatic cells of Xenopus laevis , 1991, The Journal of cell biology.

[24]  A. Bernot,et al.  DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss. , 2000, Biochimica et biophysica acta.

[25]  H. Iwahashi,et al.  Standardization of cDNA microarray technology for toxicogenomics; essential data for initiating cDNA microarray studies , 2004 .

[26]  H. Feilotter.  A Biologist’s Guide to Analysis of DNA Microarray Data. , 2002 .

[27]  H. Denis,et al.  Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis. , 1990, Nucleic acids research.

[28]  山本 時男.  Medaka (killifish) : biology and strains , 1975 .

[29]  H. Mitani,et al.  Genomic organization of ZP domain containing egg envelope genes in medaka (Oryzias latipes). , 2003, Gene.

[30]  Franco Cerrina,et al.  Gene expression analysis using oligonucleotide arrays produced by maskless photolithography. , 2002, Genome research.

[31]  M. Schartl,et al.  Genomic organization and expression of the doublesex-related gene cluster in vertebrates and detection of putative regulatory regions for DMRT1. , 2001, Genomics.

[32]  W. Wahli.  Evolution and expression of vitellogenin genes. , 1988, Trends in genetics : TIG.

[33]  T. Yamamoto,et al.  Effects of estradiol, stilbestrol and some alkyl-carbonyl androstanes upon sex differentiation in the medaka. Orvzias latipes. , 1963, General and comparative endocrinology.

[34]  C. S. Raymond,et al.  Dmrt1, a gene related to worm and fly sexual regulators, is required for mammalian testis differentiation. , 2000, Genes & development.

[35]  Shuichi Asakawa,et al.  DMY is a Y-specific DM-domain gene required for male development in the medaka fish , 2002, Nature.

[36]  H. Denis,et al.  Three genes under different developmental control encode elongation factor 1-α in Xenopus laevis , 1990 .

[37]  P. Südbeck,et al.  Male specific expression suggests role of DMRT1 in human sex determination , 2000, Mechanisms of Development.

[38]  Y. Katsu,et al.  Behavior of the components of maturation-promoting factor, cdc2 kinase and cyclin B, during oocyte maturation of goldfish. , 1993, Developmental biology.

[39]  J. Volff,et al.  Molecular cloning and characterization of DMRT genes from the medaka Oryzias latipes and the platyfish Xiphophorus maculatus. , 2002, Gene.

[40]  E. Oberdörster,et al.  Gender benders at the beach: Endocrine disruption in marine and estuarine organisms , 2001, Environmental toxicology and chemistry.

[41]  Chulwoo Lee,et al.  Choriogenin mRNA induction in male medaka, Oryzias latipes as a biomarker of endocrine disruption. , 2002, Aquatic toxicology.

[42]  Rafael A Irizarry,et al.  Exploration, normalization, and summaries of high density oligonucleotide array probe level data. , 2003, Biostatistics.

[43]  Rafael A. Irizarry,et al.  An R Package for Analyses of Affymetrix Oligonucleotide Arrays , 2003 .

[44]  C. Metcalfe,et al.  Alterations to gonadal development and reproductive success in japanese medaka (Oryzias latipes) exposed to 17α‐ethinylestradiol , 2004, Environmental toxicology and chemistry.

[45]  S. Yasumasu,et al.  Cloning of cDNA and estrogen-induced hepatic gene expression for choriogenin H, a precursor protein of the fish egg envelope (chorion). , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[46]  Masanori Seki,et al.  Life‐cycle toxicity of 4‐nonylphenol to medaka (Oryzias latipes) , 2001, Environmental toxicology and chemistry.

[47]  C. Lathers.  Endocrine Disruptors: A New Scientific Role for Clinical Pharmacologists? Impact on Human Health, Wildlife, and the Environment , 2002, Journal of clinical pharmacology.

[48]  M. Schartl,et al.  A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[49]  Y. Kohara,et al.  Large-scale analysis of the genes involved in fin regeneration and blastema formation in the medaka, Oryzias latipes , 2004, Mechanisms of Development.

[50]  Thomas H Hutchinson,et al.  Ecotoxicogenomics: the challenge of integrating genomics into aquatic and terrestrial ecotoxicology. , 2004, Aquatic toxicology.