Effect of Metalloproteinase Inhibitors on the Microtensile Bond Strength of Composite Resin to Er:YAG Laser-Irradiated Dentin.

This study evaluated the effect of matrix metalloproteinase (MMP) inhibitors - 2% (CHX) and sodium fluoride (NaF) (5000 ppm) - on microtensile bond strength (μTBS) of composite resin to Er:YAG laser-irradiated dentin after chemical degradation of the bond interface. The occlusal surface of forty sound human molars was removed exposing the dentin surface (n=10), which was polished, irradiated with Er:YAG laser, acid etched and dried. Twenty specimens were rewetted with 2% CHX (control group) and 20 were rewetted with NaF (5000 ppm). The adhesive system was applied and a 4-mm-high plateau of light-cured composite resin was built up. Resin-dentin sticks were obtained with a rectangular cross-sectional area (0.8-1 mm2) and were either stored in water at 37 ?#61616;C for 24 h or submitted to chemical degradation. For chemical degradation, they were immersed in 10% NaOCl aqueous solution for 5 h and rinsed in water for 1 h. The sticks were submitted to microtensile test in a mechanical testing machine at 0.5 mm/min until failure. Fracture pattern was analyzed using SEM. μTBS values were calculated in MPa and submitted to analysis of variance ANOVA (α=0.05). The variance analysis showed that the 'MMP inhibitor' and 'degradation' factors (p=0.214 and p=0.093, respectively) and interaction between the factors were not statistically significant (p=0.143). Mixed failure predominated in all groups. In conclusion, the 2% CHX and NaF 5000 ppm presented similar μTBS of composite resin to laser-irradiated dentin before and after chemical degradation.

[1]  F. Tay,et al.  Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability--A literature review. , 2016, Dental materials : official publication of the Academy of Dental Materials.

[2]  A. Secilmis,et al.  The use of Erbium: Yttrium-aluminum-garnet laser in cavity preparation and surface treatment: 3-year follow-up , 2015, European journal of dentistry.

[3]  T. Salo,et al.  Sodium Fluoride Inhibits MMP-2 and MMP-9 , 2014, Journal of dental research.

[4]  Silmara Aparecida Milori Corona,et al.  Long‐term chlorhexidine effect on bond strength to Er:YAG laser irradiated‐dentin , 2014, Microscopy research and technique.

[5]  L. Breschi.  Chlorhexidine application to stabilize the adhesive interface: why and how? , 2013, The journal of adhesive dentistry.

[6]  M. Toledano,et al.  Resistance to Degradation of Resin-Dentin Bonds Produced by One-Step Self-Etch Adhesives , 2012, Microscopy and Microanalysis.

[7]  D. Pashley,et al.  Chlorhexidine Inhibits the Activity of Dental Cysteine Cathepsins , 2012, Journal of dental research.

[8]  Bart Van Meerbeek,et al.  Current concepts and techniques for caries excavation and adhesion to residual dentin. , 2011, The journal of adhesive dentistry.

[9]  A. Ruggeri,et al.  Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study. , 2010, Dental materials : official publication of the Academy of Dental Materials.

[10]  R. Palma-Dibb,et al.  Assessment of in vitro methods used to promote adhesive interface degradation: a critical review. , 2007, Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.].

[11]  Christian Apel,et al.  Analysis of the interfacial micromorphology of adhesive systems in cavities prepared with Er,Cr:YSGG, Er:YAG laser and bur , 2007, Microscopy research and technique.

[12]  M F de Goes,et al.  In vivo Preservation of the Hybrid Layer by Chlorhexidine , 2007, Journal of dental research.

[13]  M. Toledano,et al.  Resistance to degradation of resin-dentin bonds using a one-step HEMA-free adhesive. , 2007, Journal of dentistry.

[14]  M F de Goes,et al.  Chlorhexidine Preserves Dentin Bond in vitro , 2007, Journal of dental research.

[15]  M. Toledano,et al.  Differential effect of in vitro degradation on resin-dentin bonds produced by self-etch versus total-etch adhesives. , 2006, Journal of biomedical materials research. Part A.

[16]  M. Ateş,et al.  Bactericidal effect of Er,Cr:YSGG laser on Streptococcus mutans. , 2006, Dental materials journal.

[17]  Denise Maria Zezell,et al.  Effect of Er:YAG laser on enamel acid resistance: Morphlogical and atomic spectrometry analysis , 2005, Lasers in surgery and medicine.

[18]  F R Tay,et al.  Chlorhexidine Arrests Subclinical Degradation of Dentin Hybrid Layers in vivo , 2005, Journal of dental research.

[19]  F. Tay,et al.  Effect of load cycling and in vitro degradation on resin-dentin bonds using a self-etching primer. , 2005, Journal of biomedical materials research. Part A.

[20]  R. Carvalho,et al.  Collagen Degradation by Host-derived Enzymes during Aging , 2004, Journal of dental research.

[21]  M. Hashimoto,et al.  Deproteinizing effects on resin-tooth bond structures. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[22]  G. Nogueira,et al.  SEM evaluation of the interaction pattern between dentin and resin after cavity preparation using ER:YAG laser. , 2003, Journal of dentistry.

[23]  F R Tay,et al.  Bonding to Er-YAG-laser-treated dentin. , 2002, Journal of dental research.

[24]  Timo Sorsa,et al.  Inhibition of the Activities of Matrix Metalloproteinases 2, 8, and 9 by Chlorhexidine , 1999, Clinical Diagnostic Laboratory Immunology.

[25]  D Heidemann,et al.  Erbium:YAG laser application in caries therapy. Evaluation of patient perception and acceptance. , 1998, Journal of dentistry.