ABSTRACT This study was designed to determine the effect on stereopsis of interocular retinal chromatic and illuminance imbalance in 30 subjects with normal binocularity. A Randot 3 Circle Stereotest, viewed through polarizing glasses only, was the control condition. In each of three additional conditions, illuminance and chromatic imbalances were created by commonly used red and green filters and neutral density filters combined with the polarizing filters. The effect of each of these experimental conditions on stereopsis was determined by comparing total stereo judgement errors on the Randot Circle Stereotest for each condition to the control condition. Total average flux through the filter combinations was held constant by adjusting the luminance level of light reflected from the target. The chromatic imbalance created by the red‐green filters significantly increased the number of stereo judgment errors (37%, p < 0.05). However, illuminance imbalance (0.2 log units) of the magnitude created by the red‐green filters did not significantly increase the number of errors (2.9%, p < 0.65). Finally, the combined illuminance and chromatic imbalance created by the red‐green filters significantly increased the error frequency (46%, p < 0.05). The chromatic imbalance caused by red‐green glasses significantly degrades stereopsis, whereas the illuminance imbalance caused by these filters has no significant effect on stereopsis.