Usage of the flow cytometer-cell sorter.

Abstract Flow cytometer-cell sorters are playing an increasingly important role in research in cellular immunology. We here review operating procedures and applications developed for our home-built flow cytometer-cell sorter which may be of general interest.

[1]  Y. Barenholz,et al.  Fluidity parameters of lipid regions determined by fluorescence polarization. , 1978, Biochimica et biophysica acta.

[2]  T. Jovin,et al.  Analysis and sorting of living cells according to deoxyribonucleic acid content. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[3]  M. Lalande,et al.  Quantitative studies on the precursors of cytotoxic lymphocytes. VI. Second signal requirements of specifically activated precursors isolated 12 h after stimulation , 1980, The Journal of experimental medicine.

[4]  H. Steen,et al.  Intracellular binding of fluorescein in lymphocytes. , 1981, Cytometry.

[5]  R. A. Phillips,et al.  Separation of cells by velocity sedimentation , 1969, Journal of cellular physiology.

[6]  R. Hill,et al.  Cell cycle distribution of chronically hypoxic cells and determination of the clonogenic potential of cells accumulated in G2 + M phases after irradiation of a solid tumor in vivo. , 1979, Cancer research.

[7]  L. Herzenberg,et al.  ANALYSIS OF CELL POPULATIONS WITH A FLUORESCENCE‐ACTIVATED CELL SORTER * , 1975, Annals of the New York Academy of Sciences.

[8]  L. Herzenberg,et al.  Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens * , 1979, Immunological reviews.

[9]  K. Shortman.  Physical procedures for the separation of animal cells. , 1972, Annual review of biophysics and bioengineering.

[10]  G. Price,et al.  Characterization of stem cells and progenitors of hemopoiesis by cell sorting. , 1979, Blood cells.

[11]  H. Steen,et al.  Flow cytometric measurement of the polarization of fluorescence from intracellular fluorescein in mammalian cells. , 1977, Biophysical journal.

[12]  R. Miller,et al.  A design for a real-time fluorescence polarization computer. , 1980, Cytometry.

[13]  L. Wheeless,et al.  Quantitative single cell analysis and sorting. , 1977, Science.

[14]  J. Meakin,et al.  A flow system adaptation of the SCM test for detection of lymphocyte response in patients with recurrent breast cancer. , 1979, Clinical immunology and immunopathology.

[15]  M. Shinitzky,et al.  Concomitant increase in antigen binding and in T cell membrane lipid viscosity induced by the lymphocyte-activating factor, LAF. , 1980, Journal of immunology.

[16]  T M Jovin,et al.  Automated cell sorting with flow systems. , 1978, Annual review of biophysics and bioengineering.

[17]  L. Cercek,et al.  Biophysical Differentiation Between Lymphocytes from Healthy Donors, Patients with Malignant Diseases and Other Disorders , 1974, British Journal of Cancer.

[18]  L. Cercek,et al.  Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: a review. , 1977, European journal of cancer.

[19]  M. McCutcheon,et al.  Measurement of cytoplasmic fluorescence depolarization of single cells in a flow system. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[20]  John G. Collard,et al.  Localization of the lipid probe 1,6-diphenyl-1,3,5 hexatriene (DPH) in intact cells by fluorescence microscopy. , 1978, Experimental cell research.

[21]  K. Shortman,et al.  The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris. Damaged cells and erythroid cells from lymphoid cell suspensions. , 1972, Journal of immunological methods.

[22]  R. Miller,et al.  Specific spontaneous cytotoxic activity in human T cell colonies. , 1980, Journal of immunology.

[23]  M. Loken.  Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  M. Lalande,et al.  Fluorescence flow analysis of lymphocyte activation using Hoechst 33342 dye. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[25]  P. Marks,et al.  Differentiation of normal and neoplastic hematopoietic cells , 1978 .

[26]  S. Latt,et al.  Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[27]  R G Sweet,et al.  Fluorescence activated cell sorting. , 1972, The Review of scientific instruments.

[28]  D. Golde.  Hematopoietic cell differentiation , 1978 .