A nano-sized Au electrode fabricated using lithographic technology for electrochemical detection of dopamine.

One big challenge of fabricating nanosensors for spatially resolved electrochemical detection of neurochemicals, such as dopamine (DA), is the difficulty to assembly nanometer-scale patternable and integrated sensors. In this work we develop a novel approach to precisely manufacture nano-Au-electrode (NAE) using lithographic fabrication technique, and characterize the NAE for DA detection. A negative photoresist, SU-8, is used as a substrate and protection layer for the 127-nm Au active sensing layer. The cross surface morphology and thickness of the Au layer are imaged by scanning electron microscopy and an interference microscopy. This NAE could be precisely controlled, repeatedly fabricated and conveniently renewed for several times. The electrochemical sensitivity and selectivity of the NAE towards DA detection are significantly higher than those of a standard Au thin-film electrode. This work demonstrates that the NAE could be used as an attractive means for electrochemically sensing and recording DA.

[1]  A. Kucernak,et al.  Fabrication of carbon microelectrodes with an effective radius of 1 nm , 2002 .

[2]  R. Adams,et al.  Nafion‐coated carbon fiber electrodes for neurochemical studies in brain tissue , 1990 .

[3]  Wei Zhou,et al.  Fabrication of size-controllable ultrasmall-disk electrode: monitoring single vesicle release kinetics at tiny structures with high spatio-temporal resolution. , 2009, Biosensors & bioelectronics.

[4]  W. Tseng,et al.  Phosphate-modified TiO2 nanoparticles for selective detection of dopamine, levodopa, adrenaline, and catechol based on fluorescence quenching. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[5]  S. Andreescu,et al.  Amperometric detection of dopamine in vivo with an enzyme based carbon fiber microbiosensor. , 2010, Analytical chemistry.

[6]  Barry Condron,et al.  Detection of endogenous dopamine changes in Drosophila melanogaster using fast-scan cyclic voltammetry. , 2009, Analytical chemistry.

[7]  D. Arrigan,et al.  Dopamine voltammetry at overoxidised polyindole electrodes , 2004 .

[8]  J. A. Jankowski,et al.  Zones of exocytotic release on bovine adrenal medullary cells in culture. , 1994, The Journal of biological chemistry.

[9]  Suw Young Ly,et al.  Detection of dopamine in the pharmacy with a carbon nanotube paste electrode using voltammetry. , 2006, Bioelectrochemistry.

[10]  B. J. Venton,et al.  Carbon nanotube-modified microelectrodes for simultaneous detection of dopamine and serotonin in vivo. , 2007, The Analyst.

[11]  W. Dehaen,et al.  Gold electrode incorporating corrole as an ion-channel mimetic sensor for determination of dopamine. , 2009, Analytical chemistry.

[12]  Kiyofumi Yamada,et al.  Role of the mesotelencephalic dopamine system in learning and memory processes in the rat. , 2003, European journal of pharmacology.

[13]  D. Pang,et al.  A method for the fabrication of low-noise carbon fiber nanoelectrodes. , 2001, Analytical chemistry.

[14]  Robert T Kennedy,et al.  Microfluidic chip for high efficiency electrophoretic analysis of segmented flow from a microdialysis probe and in vivo chemical monitoring. , 2009, Analytical chemistry.

[15]  P. Shizgal,et al.  Dynamic changes in dopamine tone during self-stimulation of the ventral tegmental area in rats , 2009, Behavioural Brain Research.

[16]  R. Wightman,et al.  Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo. , 2003, Clinical chemistry.

[17]  R. Wightman,et al.  Characterization of amperometry for in vivo measurement of dopamine dynamics in the rat brain. , 1994, Talanta.

[18]  W. Zhang,et al.  Fabrication, characterization, and potential application of carbon fiber cone nanometer-size electrodes. , 1996, Analytical chemistry.

[19]  I. Watanabe,et al.  ACTIVATION OF A GOLD ELECTRODE BY ELECTROCHEMICAL OXIDATION-REDUCTION PRETREATMENT IN HYDROCHLORIC ACID , 1991 .

[20]  M. Mirski,et al.  Patternable nanowire sensors for electrochemical recording of dopamine. , 2009, Analytical chemistry.

[21]  Kelly L. Adams,et al.  Highly sensitive detection of exocytotic dopamine release using a gold-nanoparticle-network microelectrode. , 2011, Analytical chemistry.

[22]  Zhuan Zhou,et al.  “Kiss-and-Run” Glutamate Secretion in Cultured and Freshly Isolated Rat Hippocampal Astrocytes , 2005, The Journal of Neuroscience.

[23]  T. Fukushima,et al.  A method to evaluate the renin-angiotensin system in rat renal cortex using a microdialysis technique combined with HPLC-fluorescence detection. , 2002, Analytical Chemistry.

[24]  Richard M. Crooks,et al.  Electrochemistry Using Single Carbon Nanotubes , 1999 .

[25]  N. Lewis,et al.  Fabrication and Use of Nanometer-Sized Electrodes in Electrochemistry , 1990, Science.

[26]  Yanming Liu,et al.  Determination of catecholamines by CE with direct chemiluminescence detection , 2007, Electrophoresis.

[27]  A. Ewing,et al.  Dopamine concentration in the cytoplasmic compartment of single neurons determined by capillary electrophoresis , 1990, Journal of Neuroscience Methods.

[28]  S. Furlanetto,et al.  Simultaneous liquid chromatographic analysis of catecholamines and 4-hydroxy-3-methoxyphenylethylene glycol in human plasma. Comparison of amperometric and coulometric detection. , 2004, Journal of chromatography. A.

[29]  Marcelo F de Oliveira,et al.  Electrooxidation and Determination of Dopamine Using a Nafion®-Cobalt Hexacyanoferrate Film Modified Electrode , 2008, Sensors.