What is next for Brillouin microscopy in biology and medicine?

Brillouin microscopy is an emerging technique in biomedical imaging capable of non-invasive assessing viscoelastic properties on a microscopic scale. In this report, we outline the latest developments in Brillouin spectroscopy instrumentation and applications in an attempt to anticipate the future impact areas of this new imaging modality.

[1]  Rob Krams,et al.  Quantification of plaque stiffness by Brillouin microscopy in experimental thin cap fibroatheroma , 2015, Journal of The Royal Society Interface.

[2]  Carl Paterson,et al.  Elastic suppression in Brillouin imaging by destructive interference , 2015 .

[3]  Giuseppe Antonacci,et al.  Biomechanics of subcellular structures by non-invasive Brillouin microscopy , 2016, Scientific Reports.

[4]  Matt Clark,et al.  High resolution 3D imaging of living cells with sub-optical wavelength phonons , 2016, Scientific Reports.

[5]  Zhaokai Meng,et al.  Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy , 2016, Journal of biophotonics.

[6]  S. Yun,et al.  Confocal Brillouin microscopy for three-dimensional mechanical imaging. , 2007, Nature photonics.

[7]  Itay Remer,et al.  Background-free Brillouin spectroscopy in scattering media at 780  nm via stimulated Brillouin scattering. , 2016, Optics letters.

[8]  Carl Paterson,et al.  Spectral broadening in Brillouin imaging , 2013 .

[9]  Itay Remer,et al.  High-speed stimulated Brillouin scattering spectroscopy at 780 nm , 2016 .

[10]  Giuliano Scarcelli,et al.  Translating Ocular Biomechanics into Clinical Practice: Current State and Future Prospects , 2015, Current eye research.

[11]  Pilhan Kim,et al.  In vivo measurement of age-related stiffening in the crystalline lens by Brillouin optical microscopy. , 2011, Biophysical journal.

[12]  Marlan O Scully,et al.  Chemical analysis of molecular species through turbid medium. , 2014, Analytical chemistry.

[13]  Vladislav V. Yakovlev,et al.  Nonlinear optical susceptibility measurements of solutions using third-harmonic generation on the interface , 2003 .

[14]  Rajan Arora,et al.  Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing. , 2011, Journal of biomedical optics.

[15]  Vladislav V. Yakovlev,et al.  Background clean-up in Brillouin microspectroscopy of scattering medium. , 2014, Optics express.

[16]  Vladislav V. Yakovlev,et al.  Seeing cells in a new light: a renaissance of Brillouin spectroscopy , 2016 .

[17]  Marlan O Scully,et al.  Dual Raman-Brillouin Microscope for Chemical and Mechanical Characterization and Imaging. , 2015, Analytical chemistry.

[18]  S. Yun,et al.  Brillouin optical microscopy for corneal biomechanics. , 2012, Investigative ophthalmology & visual science.

[19]  S. Saltiel,et al.  Quantitative characterization of aqueous solutions probed by the third-harmonic generation microscopy. , 2004, Journal of structural biology.

[20]  Vladislav V. Yakovlev,et al.  Stimulated Brillouin Scattering Microscopic Imaging , 2015, Scientific Reports.

[21]  N Stone,et al.  Mechanical mapping with chemical specificity by confocal Brillouin and Raman microscopy. , 2014, The Analyst.

[22]  Giuliano Scarcelli,et al.  Etalon filters for Brillouin microscopy of highly scattering tissues. , 2016, Optics express.

[23]  Zhaokai Meng,et al.  Flow cytometry using Brillouin imaging and sensing via time-resolved optical (BISTRO) measurements. , 2015, The Analyst.

[24]  Zhaokai Meng,et al.  Precise Determination of Brillouin Scattering Spectrum Using a Virtually Imaged Phase Array (VIPA) Spectrometer and Charge-Coupled Device (CCD) Camera , 2016, Applied spectroscopy.

[25]  O. Stachs,et al.  Spatially resolved Brillouin spectroscopy to determine the rheological properties of the eye lens , 2011, Biomedical optics express.

[26]  Vladislav V. Yakovlev,et al.  Impulsive Brillouin microscopy , 2017 .

[27]  L. Brillouin.  Diffusion de la lumière et des rayons X par un corps transparent homogène - Influence de l'agitation thermique , 1922 .

[28]  Kareem Elsayad,et al.  Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging , 2016, Science Signaling.

[29]  J. Ophir,et al.  Elastography: A Quantitative Method for Imaging the Elasticity of Biological Tissues , 1991, Ultrasonic imaging.

[30]  J. Rao,et al.  Nanomechanical analysis of cells from cancer patients. , 2007, Nature nanotechnology.

[31]  Vladislav V. Yakovlev,et al.  Optimizing signal collection efficiency of the VIPA-based Brillouin spectrometer , 2015 .

[32]  Gary D Noojin,et al.  Assessment of tissue heating under tunable near-infrared radiation , 2014, Journal of biomedical optics.

[33]  Giuliano Scarcelli,et al.  Shear Brillouin light scattering microscope. , 2016, Optics express.

[34]  Giuliano Scarcelli,et al.  Line-scanning Brillouin microscopy for rapid non-invasive mechanical imaging , 2016, Scientific Reports.

[35]  Giancarlo Ruocco,et al.  Breaking the Contrast Limit in Single-Pass Fabry-Pérot Spectrometers , 2016 .

[36]  Vladislav V. Yakovlev,et al.  Nonlinear optical properties of collagen in solution , 2005 .

[37]  S. Yun,et al.  High-finesse sub-GHz-resolution spectrometer employing VIPA etalons of different dispersion. , 2015, Optics letters.

[38]  William J. Polacheck,et al.  Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy , 2015, Nature Methods.