The inflammatory response in asthma exacerbation: changes in circulating eosinophils, basophils and their progenitors

Circulating eosinophils, basophils and eosinophil/basophil (Eo/B) progenitors were examined in 12 patients at the time of an exacerbation of asthma accompanied by sputum eosinophilia and after resolution of the exacerbation with inhaled corticosteroid treatment. Differential counts were performed and peripheral blood non‐adherent mononuclear cells were cultured for 14 days in methyl‐cellulose to determine the number of Eo/B and granulocyte‐macrophage (GM) colonies without knowledge of the clinical conditions or findings. With resolution of the asthma exacerbation on beclomethasone therapy, there were significant falls in circulating eosinophils, basophils and Eo/B colonies whereas GM colonies were unchanged. To elucidate whether the observed changes could be due to systemic absorption or local action of inhaled corticosteroid, seven subjects with allergic rhinitis and no current evidence of lower airway inflammation (no symptoms of asthma and normal methacholine airway responsiveness) received 14 days' treatment with the same dose of inhaled beclomethasone or of placebo in a double‐blind randomized cross‐over study. No significant changes in airway function or in circulating cell counts were observed. The results suggest reduced production of eosinophils and basophils after the resolution of an exacerbation of asthma. This effect may be due to reduced levels of airway‐derived eosinophil‐basophil growth and differentiation factors.

[1]  D. Linch,et al.  Haemopoietic growth factors. , 1990, The Quarterly journal of medicine.

[2]  J. Denburg,et al.  Cellular characteristics of sputum from patients with asthma and chronic bronchitis. , 1989, Thorax.

[3]  A. Sheffer,et al.  Interleukin 5 and phenotypically altered eosinophils in the blood of patients with the idiopathic hypereosinophilic syndrome , 1989, The Journal of experimental medicine.

[4]  K. Saito,et al.  T cells from eosinophilic patients produce interleukin-5 with interleukin-2 stimulation. , 1989, Blood.

[5]  P. Valent,et al.  Interleukin-3 is a differentiation factor for human basophils. , 1989, Blood.

[6]  L. Ashman,et al.  Mast cell typing: demonstration of a distinct hematopoietic cell type and evidence for immunophenotypic relationship to mononuclear phagocytes. , 1989, Blood.

[7]  J. Denburg,et al.  Basophil mast cell and eosinophil growth and differentiation factors in human allergic disease , 1989, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[8]  D. Metcalf.  HAEMOPOIETIC GROWTH FACTORS 1 , 1989, The Lancet.

[9]  P. Valent,et al.  The in vivo effects of recombinant human interleukin-3: demonstration of basophil differentiation factor, histamine-producing activity, and priming of GM-CSF-responsive progenitors in nonhuman primates. , 1989, Blood.

[10]  J. Nadel,et al.  Mast cell tryptase causes airway smooth muscle hyperresponsiveness in dogs. , 1989, The Journal of clinical investigation.

[11]  P. Sehgal,et al.  Human IL-3 and GM-CSF act synergistically in stimulating hematopoiesis in primates. , 1988, Science.

[12]  S. Cannistra,et al.  Regulation of the production and function of granulocytes and monocytes. , 1988, Seminars in hematology.

[13]  W. Owen,et al.  Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3. , 1988, The Journal of clinical investigation.

[14]  M. Socinski,et al.  GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR EXPANDS THE CIRCULATING HAEMOPOIETIC PROGENITOR CELL COMPARTMENT IN MAN , 1988, The Lancet.

[15]  C. Corrigan,et al.  T LYMPHOCYTE ACTIVATION IN ACUTE SEVERE ASTHMA , 1988, The Lancet.

[16]  S. Wenzel,et al.  Activation of pulmonary mast cells by bronchoalveolar allergen challenge. In vivo release of histamine and tryptase in atopic subjects with and without asthma. , 1988, The American review of respiratory disease.

[17]  R. Schleimer,et al.  Adherence of human basophils to cultured umbilical vein endothelial cells. , 1988, The Journal of clinical investigation.

[18]  A. Donnenberg,et al.  Selective differentiation and proliferation of hematopoietic cells induced by recombinant human interleukins. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[19]  N. Flavahan,et al.  Human eosinophil major basic protein causes hyperreactivity of respiratory smooth muscle. Role of the epithelium. , 1988, The American review of respiratory disease.

[20]  A. Wardlaw,et al.  Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma. Relationship to bronchial hyperreactivity. , 1988, The American review of respiratory disease.

[21]  P. O'Byrne,et al.  Bronchoalveolar cell profiles of asthmatic and nonasthmatic subjects. , 1987, The American review of respiratory disease.

[22]  J. Denburg,et al.  Metachromatic cell progenitors and specific growth and differentiation factors in human nasal mucosa and polyps. , 1987, The American review of respiratory disease.

[23]  M. Dietrich,et al.  Eosinophil production in human bone‐marrow cultures induced by 80–85 kDa serum component(s) of patients with eosinophilia , 1987, British journal of haematology.

[24]  P. O'Byrne,et al.  Asthma and the role of inflammation. , 1986, European journal of respiratory diseases. Supplement.

[25]  J. Denburg,et al.  Increased numbers of circulating basophil progenitors in atopic patients. , 1985, The Journal of allergy and clinical immunology.

[26]  J. Denburg,et al.  Heterogeneity of human peripheral blood eosinophil-type colonies: evidence for a common basophil-eosinophil progenitor. , 1985, Blood.

[27]  E. Brown,et al.  Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. , 1985, Science.

[28]  H. Sluiter,et al.  Bronchoalveolar eosinophilia during allergen-induced late asthmatic reactions. , 1985, The American review of respiratory disease.

[29]  E. Juniper,et al.  Histamine inhalation tests: inhalation of aerosol via a facemask versus a valve box with mouthpiece. , 1984, Thorax.

[30]  E. Zanjani,et al.  Pulmonary carcinoma with eosinophilia. Demonstration of a tumor-derived eosinophilopoietic factor. , 1983, The New England journal of medicine.

[31]  N. Bersch,et al.  Production of erythroid-potentiating activity by a human T-lymphoblast cell line. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[32]  M. Hills,et al.  The two-period cross-over clinical trial. , 1979, British journal of clinical pharmacology.

[33]  F. Hargreave,et al.  Bronchial reactivity to inhaled histamine: a method and clinical survey , 1977, Clinical allergy.

[34]  Gordon My,et al.  The effect of peripheral blood contamination on colony yield from human bone marrow aspirates. , 1977 .

[35]  D. Cockcroft,et al.  Beclomethasone dipropionate aerosol in allergic rhinitis. , 1976, Canadian Medical Association journal.

[36]  E D Robin,et al.  Total eosinophil counts in the management of bronchial asthma. , 1975, The New England journal of medicine.

[37]  P. Gell,et al.  Clinical aspects of immunology , 1968 .