Mobility-Selected Ion Trapping and Enrichment Using Structures for Lossless Ion Manipulations.

The integration of ion mobility spectrometry (IMS) with mass spectrometry (MS) and the ability to trap ions in IMS-MS measurements is of great importance for performing reactions, accumulating ions, and increasing analytical measurement sensitivity. The development of Structures for Lossless Ion Manipulations (SLIM) offers the potential for ion manipulations in an extended and more effective manner, while opening opportunities for many more complex sequences of manipulations. Here, we demonstrate an ion separation and trapping module and a method based upon SLIM that consists of a linear mobility ion drift region, a switch/tee and a trapping region that allows the isolation and accumulation of mobility-separated species. The operation and optimization of the SLIM switch/tee and trap are described and demonstrated for the enrichment of the low abundance ions. A linear improvement in ion intensity was observed with the number of trapping/accumulation events using the SLIM trap, illustrating its potential for enhancing the sensitivity of low abundance or targeted species.

[1]  Yehia M. Ibrahim,et al.  Rectangular Ion Funnel: A New Ion Funnel Interface for Structures for Lossless Ion Manipulations , 2014, Analytical chemistry.

[2]  Zheng Ouyang,et al.  Preparing Protein Microarrays by Soft-Landing of Mass-Selected Ions , 2003, Science.

[3]  R. Cooks,et al.  Preparative linear ion trap mass spectrometer for separation and collection of purified proteins and peptides in arrays using ion soft landing. , 2004, Analytical Chemistry.

[4]  S. A. McLuckey,et al.  Collision-induced dissociation (CID) of peptides and proteins. , 2005, Methods in enzymology.

[5]  Yehia M. Ibrahim,et al.  Mobility-Resolved Ion Selection in Uniform Drift Field Ion Mobility Spectrometry/Mass Spectrometry: Dynamic Switching in Structures for Lossless Ion Manipulations , 2014, Analytical Chemistry.

[6]  Yehia M. Ibrahim,et al.  Characterization of Ion Dynamics in Structures for Lossless Ion Manipulations , 2014, Analytical chemistry.

[7]  Yehia M. Ibrahim,et al.  Ion Trapping, Storage, and Ejection in Structures for Lossless Ion Manipulations. , 2015, Analytical chemistry.

[8]  Yehia M. Ibrahim,et al.  Simulation of Electric Potentials and Ion Motion in Planar Electrode Structures for Lossless Ion Manipulations (SLIM) , 2014, Journal of The American Society for Mass Spectrometry.

[9]  Yehia M. Ibrahim,et al.  Experimental Evaluation and Optimization of Structures for Lossless Ion Manipulations for Ion Mobility Spectrometry with Time-of-Flight Mass Spectrometry , 2014, Analytical chemistry.

[10]  Yehia M. Ibrahim,et al.  Enhanced ion utilization efficiency using an electrodynamic ion funnel trap as an injection mechanism for ion mobility spectrometry. , 2008, Analytical chemistry.

[11]  A. Marshall,et al.  Stored waveform inverse Fourier transform axial excitation/ejection for quadrupole ion trap mass spectrometry. , 1993, Analytical chemistry.

[12]  J. Schwartz,et al.  High resolution parent-ion selection/isolation using a quadrupole ion-trap mass spectrometer , 1992 .

[13]  Yehia M. Ibrahim,et al.  Ion manipulations in structures for lossless ion manipulations (SLIM): computational evaluation of a 90° turn and a switch. , 2015, The Analyst.

[14]  Yehia M. Ibrahim,et al.  Pulsed multiple reaction monitoring approach to enhancing sensitivity of a tandem quadrupole mass spectrometer. , 2011, Analytical chemistry.

[15]  R. Cooks,et al.  Selective Injection and Isolation of Ions in Quadrupole Ion Trap Mass Spectrometry Using Notched Waveforms Created Using the Inverse Fourier Transform , 1994 .

[16]  F. J. Knorr,et al.  Fourier transform ion mobility spectrometry. , 1985, Analytical chemistry.

[17]  T. Wyttenbach,et al.  Design of a new electrospray ion mobility mass spectrometer , 2001 .

[18]  Richard D. Smith,et al.  Ion discrimination during ion accumulation in a quadrupole interface external to a Fourier transform ion cyclotron resonance mass spectrometer , 2001 .

[19]  Pietro Traldi,et al.  Rapid Commun. Mass Spectrom.10. 1629-1637 (1996) Matrix-assisted Laser Desorption/Ionisation Mass Spectrometry in Milk Science , 1997 .

[20]  Yehia M. Ibrahim,et al.  Ion funnel trap interface for orthogonal time-of-flight mass spectrometry. , 2007, Analytical chemistry.