Comparison of Fenestra LC, ExiTron nano 6000, and ExiTron nano 12000 for micro-CT imaging of liver and spleen in mice.

RATIONALE AND OBJECTIVES The purpose of this study was to compare different contrast agents for longitudinal liver and spleen imaging in a mouse model of liver metastasis. MATERIALS AND METHODS Mice developing liver metastases underwent longitudinal micro-computed tomography imaging after injection of Fenestra LC, ExiTron nano 6000, or ExiTron nano 12000. Elimination times and contrast enhancement of liver and spleen were compared. RESULTS For all contrast agents, liver contrast peaked at approximately 4 hours and spleen contrast at 48 hours postinjection. A single dose of 100 μL of ExiTron nano 6000 or 12000 resulted in longstanding enhancement of liver and spleen tissue for longer than 3 weeks, whereas repeated injections of 400 μL of Fenestra LC were required to retain contrast at acceptable levels and allowed imaging of the liver/spleen for up to 2 and 9 days, respectively. CONCLUSION Both ExiTron nano agents provide longer and stronger contrast enhancement of liver and spleen compared to Fenestra LC, and they do so at a 75% lower injection volume in mice.

[1]  A. Tischler,et al.  MicroCT for high‐resolution imaging of ectopic pheochromocytoma tumors in the liver of nude mice , 2006, International journal of cancer.

[2]  Jamey P Weichert,et al.  Imaging of murine liver tumor using microCT with a hepatocyte-selective contrast agent: accuracy is dependent on adequate contrast enhancement. , 2004, The Journal of surgical research.

[3]  Antoine Geissbuhler,et al.  Improved Visualization of Vessels and Hepatic Tumors by Micro-Computed Tomography (CT) Using Iodinated Liposomes , 2007, Investigative radiology.

[4]  Rudi Deklerck,et al.  Time-Course of Contrast Enhancement in Spleen and Liver with Exia 160, Fenestra LC, and VC , 2009, Molecular Imaging and Biology.

[5]  Maria Drangova,et al.  Contrast-Enhanced Microcomputed Tomography Using Intraperitoneal Contrast Injection for the Assessment of Tumor-Burden in Liver Metastasis Models , 2008, Investigative radiology.

[6]  R. Counsell,et al.  Polyiodinated triglyceride lipid emulsions for use as hepatoselective contrast agents in CT: effects of physicochemical properties on biodistribution and imaging profiles. , 2000, Investigative radiology.

[7]  M. Neumaier,et al.  Monoclonal anti-idiotype antibody 6G6.C4 fused to GM-CSF is capable of breaking tolerance to carcinoembryonic antigen (CEA) in CEA-transgenic mice. , 2005, Cancer research.

[8]  C. Groden,et al.  Three-Dimensional In Vivo Imaging of the Murine Liver: A Micro-Computed Tomography-Based Anatomical Study , 2012, PloS one.

[9]  R. Counsell,et al.  Polyiodinated triglyceride analogs as potential computed tomography imaging agents for the liver. , 1995, Journal of medicinal chemistry.

[10]  C. Groden,et al.  Evaluation of a Continuous-Rotation, High-Speed Scanning Protocol for Micro-Computed Tomography , 2011, Journal of computer assisted tomography.

[11]  David B. Stout,et al.  MicroCT Liver Contrast Agent Enhancement Over Time, Dose, and Mouse Strain , 2007, Molecular Imaging and Biology.

[12]  P. Choyke,et al.  Noninvasive monitoring of a murine model of metastatic pheochromocytoma: A comparison of contrast‐enhanced microCT and nonenhanced MRI , 2009, Journal of magnetic resonance imaging : JMRI.

[13]  R. Counsell,et al.  Formulation of Polyiodinated Triglyceride Analogues in a Chylomicron Remnant-Like Liver-Selective Delivery Vehicle , 1996, Pharmaceutical Research.

[14]  Michael Neumaier,et al.  Micro-CT Based Experimental Liver Imaging Using a Nanoparticulate Contrast Agent: A Longitudinal Study in Mice , 2011, PloS one.

[15]  C. Groden,et al.  High-Speed Single-Breath-Hold Micro-Computed Tomography of Thoracic and Abdominal Structures in Mice Using a Simplified Method for Intubation , 2010, Journal of computer assisted tomography.

[16]  F. Lee,et al.  Imaging Efficacy of a Hepatocyte-Selective Polyiodinated Triglyceride for Contrast-Enhanced Computed Tomography , 2001, American journal of therapeutics.

[17]  R. Counsell,et al.  Physicochemical characterization of a synthetic lipid emulsion for hepatocyte-selective delivery of lipophilic compounds: application to polyiodinated triglycerides as contrast agents for computed tomography. , 1996, Journal of pharmaceutical sciences.

[18]  Karel Pacak,et al.  In vivo micro-CT imaging of liver lesions in small animal models. , 2010, Methods.

[19]  Christoph Groden,et al.  Application of micro-CT in small animal imaging. , 2010, Methods.

[20]  M. Janier,et al.  In-vivo high-resolution X-ray microtomography for liver and spleen tumor assessment in mice. , 2007, Contrast media & molecular imaging.

[21]  C. Groden,et al.  Double-contrast micro-CT colonoscopy in live mice , 2011, International Journal of Colorectal Disease.

[22]  W. Zimmermann,et al.  Mice transgenic for the human carcinoembryonic antigen gene maintain its spatiotemporal expression pattern. , 1994, Cancer research.