A self-assembled conjugated micelle with improved sensitivity for monitoring alkaline phosphatase activity

Abstract An amphiphilic coumarin derivative which forms a π-extended micelle conformation 1 was designed and developed. The exciton efficiently migrates throughout the coumarin aggregates of 1, showing amplified fluorescence quenching in the presence of Cu2+ ions. The 1-Cu2+ complex displays a highly sensitive response to pyrophosphate (PPi), leading to 80% fluorescence recovery. The activity of alkaline phosphatase (ALP) was monitored by a real-time assay where a solution containing 1, Cu2+, and PPi in aqueous solution exhibits a sensitive turn-off fluorescence response to ALP.

[1]  S. P. Fletcher,et al.  Potential for minimal self-replicating systems in a dynamic combinatorial library of equilibrating imines , 2017 .

[2]  Jianlong Wang,et al.  Novel coumarin-based containing denrons selective fluorescent chemosesor for sequential recognition of Cu2+ and PPi , 2019, Tetrahedron.

[3]  S. Maiti,et al.  Dissipative self-assembly of vesicular nanoreactors. , 2016, Nature chemistry.

[4]  Jong‐In Hong,et al.  Highly sensitive chemosensor for detection of PPi with improved detection limit , 2011 .

[5]  Qiang Zhao,et al.  Highly efficient organic photosensitizer with aggregation-induced emission for imaging-guided photodynamic ablation of cancer cells , 2018, Tetrahedron Letters.

[6]  R. Schröder,et al.  Truxene-Based Hyperbranched Conjugated Polymers: Fluorescent Micelles Detect Explosives in Water. , 2017, ACS applied materials & interfaces.

[7]  Wenli Zhang,et al.  A fluorescent chemosensor for Cu2+ ions and its application in cell imaging , 2017 .

[8]  A. Ray,et al.  An acac-BODIPY dye as a reversible “ON-OFF-ON” fluorescent sensor for Cu2+ and S2- ions based on displacement approach , 2017 .

[9]  Jingjing Deng,et al.  Real-time ratiometric fluorescent assay for alkaline phosphatase activity with stimulus responsive infinite coordination polymer nanoparticles. , 2015, Analytical chemistry.

[10]  D. Liang,et al.  A coumarin based highly selective fluorescent chemosensor for sequential recognition of Cu2+ and PPi , 2018, Tetrahedron Letters.

[11]  Ricardo Mallavia,et al.  Fluorescent Biosensor for Phosphate Determination Based on Immobilized Polyfluorene-Liposomal Nanoparticles Coupled with Alkaline Phosphatase. , 2017, ACS applied materials & interfaces.

[12]  K. Schanze,et al.  Pyrophosphate Sensor Based on Principal Component Analysis of Conjugated Polyelectrolyte Fluorescence , 2016, ACS omega.

[13]  X. Ren,et al.  A Turn-On Fluorescent Sensor for Selective and Sensitive Detection of Alkaline Phosphatase Activity with Gold Nanoclusters Based on Inner Filter Effect. , 2017, ACS Applied Materials and Interfaces.