Cyclic strain improves strength and function of a collagen-based tissue-engineered vascular media.

Tissue-engineered blood vessels may provide a solution to the lack of suitable blood vessels for coronary and peripheral vessel bypass grafting. Cyclic strain can be used to provide a more physiological environment that may result in tissue that more closely resembles native artery. In this study, cyclic strain is applied to a collagen-based, tissue-engineered vascular medium. An increased culture time was used to allow the tissue to adhere to the silastic sleeve and to eliminate longitudinal compaction. Cyclic strain improved tissue strength through increased collagen content as well as some radial tissue compaction. Mechanical stimulation promoted a more contractile phenotype and led to a greater contractile response to the vasoconstrictor endothelin-1.

[1]  Robert M Nerem,et al.  Mechanical strain-stimulated remodeling of tissue-engineered blood vessel constructs. , 2003, Tissue engineering.

[2]  R. Sodian,et al.  Tissue engineering of small caliber vascular grafts. , 2001, European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery.

[3]  S. Andreadis,et al.  Fibrin-based tissue-engineered blood vessels: differential effects of biomaterial and culture parameters on mechanical strength and vascular reactivity. , 2005, Tissue engineering.

[4]  Construction of functional soft tissues from premodulated smooth muscle cells using a bioreactor system. , 2006, Artificial organs.

[5]  Wei Liu,et al.  Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor. , 2008, Biomaterials.

[6]  James A. Russell,et al.  Engineering of fibrin-based functional and implantable small-diameter blood vessels. , 2005 .

[7]  L. Niklason,et al.  Effect of pulse rate on collagen deposition in the tissue-engineered blood vessel. , 2003, Tissue engineering.

[8]  Zong-Lai Jiang,et al.  Frequency-Dependent Phenotype Modulation of Vascular Smooth Muscle Cells under Cyclic Mechanical Strain , 2007, Journal of Vascular Research.

[9]  James A Russell,et al.  Functional tissue-engineered blood vessels from bone marrow progenitor cells. , 2007, Cardiovascular research.

[10]  J. Nishimura,et al.  Contractile Properties of the Cultured Vascular Smooth Muscle Cells: The Crucial Role Played by RhoA in the Regulation of Contractility , 2005, Circulation research.

[11]  A. Ratcliffe.  Tissue engineering of vascular grafts. , 2000, Matrix biology : journal of the International Society for Matrix Biology.

[12]  Robert M. Nerem,et al.  The Role of Matrix Metalloproteinase-2 in the Remodeling of Cell-Seeded Vascular Constructs Subjected to Cyclic Strain , 2001, Annals of Biomedical Engineering.

[13]  Jennifer L. West,et al.  Physiologic Pulsatile Flow Bioreactor Conditioning of Poly(ethylene glycol)-based Tissue Engineered Vascular Grafts , 2007, Annals of Biomedical Engineering.

[14]  L. Germain,et al.  Tissue-engineered human vascular media produced in vitro by the self-assembly approach present functional properties similar to those of their native blood vessels. , 2006, Tissue engineering.

[15]  Robert T. Tranquillo,et al.  Long-Term Cyclic Distention Enhances the Mechanical Properties of Collagen-Based Media-Equivalents , 2003, Annals of Biomedical Engineering.

[16]  Konstantin G. Birukov,et al.  Stretch affects phenotype and proliferation of vascular smooth muscle cells , 1995, Molecular and Cellular Biochemistry.

[17]  Jan Feijen,et al.  Biological characterisation of vascular grafts cultured in a bioreactor. , 2006, Biomaterials.

[18]  S. Andreadis,et al.  Composite Fibrin Scaffolds Increase Mechanical Strength and Preserve Contractility of Tissue Engineered Blood Vessels , 2008, Pharmaceutical Research.

[19]  L. Germain,et al.  Tissue-Engineered Human Vascular Media With a Functional Endothelin System , 2005, Circulation.

[20]  M S Conte,et al.  The ideal small arterial substitute: a search for the Holy Grail? , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[21]  Guillermo A. Ameer,et al.  In Vitro Characterization of a Compliant Biodegradable Scaffold with a Novel Bioreactor System , 2007, Annals of Biomedical Engineering.

[22]  Jacqueline T Johanas,et al.  Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts. , 2009, Biomaterials.

[23]  Lucie Germain,et al.  Adventitia contribution in vascular tone: insights from adventitia‐derived cells in a tissue‐engineered human blood vessel , 2006, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[24]  Jan P. Stegemann,et al.  Phenotype Modulation in Vascular Tissue Engineering Using Biochemical and Mechanical Stimulation , 2003, Annals of Biomedical Engineering.

[25]  L. Germain,et al.  A human tissue‐engineered vascular media: a new model for pharmacological studies of contractile responses , 2001, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[26]  J. Cha,et al.  Time-dependent modulation of alignment and differentiation of smooth muscle cells seeded on a porous substrate undergoing cyclic mechanical strain. , 2006, Artificial organs.

[27]  Robert T Tranquillo,et al.  Cyclic distension of fibrin-based tissue constructs: Evidence of adaptation during growth of engineered connective tissue , 2008, Proceedings of the National Academy of Sciences.

[28]  David J. Mooney,et al.  Cyclic mechanical strain regulates the development of engineered smooth muscle tissue , 1999, Nature Biotechnology.

[29]  R M Nerem,et al.  Vascular tissue engineering. , 2001, Annual review of biomedical engineering.

[30]  R. Haut,et al.  Mechanical properties of the canine aorta following hypercholesterolemia. , 1980, Journal of Biomechanical Engineering.

[31]  C. Hunt,et al.  Cryopreservation of the common carotid artery of the rabbit. , 1994, Cryobiology.

[32]  H. Ives,et al.  Mechanical strain increases smooth muscle and decreases nonmuscle myosin expression in rat vascular smooth muscle cells. , 1996, Circulation research.

[33]  K. Webb,et al.  Variation of cyclic strain parameters regulates development of elastic modulus in fibroblast/substrate constructs , 2008, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[34]  P. Hagen,et al.  Remodeling of an acellular collagen graft into a physiologically responsive neovessel , 1999, Nature Biotechnology.

[35]  L. Niklason,et al.  Feasibility of vitrification as a storage method for tissue-engineered blood vessels. , 2006, Tissue engineering.

[36]  Michael J. Taylor,et al.  Vitreous cryopreservation maintains the function of vascular grafts , 2000, Nature Biotechnology.

[37]  R Langer,et al.  Functional arteries grown in vitro. , 1999, Science.

[38]  C. Hunt,et al.  Cryopreservation of the common carotid artery of the rabbit: optimization of dimethyl sulfoxide concentration and cooling rate. , 1995, Cryobiology.

[39]  Frank P. T. Baaijens,et al.  Effect of Strain Magnitude on the Tissue Properties of Engineered Cardiovascular Constructs , 2007, Annals of Biomedical Engineering.

[40]  F A Auger,et al.  A completely biological tissue‐engineered human blood vessel , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[41]  Dany J. Munoz-Pinto,et al.  Impact of endothelial cells and mechanical conditioning on smooth muscle cell extracellular matrix production and differentiation. , 2009, Tissue engineering. Part A.

[42]  Robert M. Nerem,et al.  Dynamic Mechanical Conditioning of Collagen-Gel Blood Vessel Constructs Induces Remodeling In Vitro , 2000, Annals of Biomedical Engineering.