Experimental immunisation of hamsters with I i po po I ysa c c h a r i d e a n t i g e n s of L eptospira interrogans

Summary. Hamsters were immunised with leptospiral lipopolysaccharide (LPS) or the polysaccharide (PS) fraction of LPS from Leptospira interrogans serovar copenhageni and the antibody responses were measured by agglutination tests. Maximum titres were observed approximately 6 weeks after immunisation and protection against lethal challenge with the homologous strain was afforded by immunisation with as little as 2-5 pg of LPS or PS. All animals produced IgM agglutinins but a higher proportion of animals immunised with PS produced IgG agglutinins than did those immunised with LPS. Immunisation of guinea-pigs with autoclaved PS showed that the preparation retained some but not all of its immunogenic activity.

[1]  B. Adler,et al.  Antigens recognised by the human immune response to infection with Leptospira interrogans serovar hardjo. , 1988, Journal of medical microbiology.

[2]  S Faine,et al.  Opsonic monoclonal antibodies against lipopolysaccharide antigens of Leptospira interrogans serovar hardjo. , 1987, Journal of medical microbiology.

[3]  S Faine,et al.  A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea pigs against leptospirosis. , 1986, Journal of medical microbiology.

[4]  B. Adler,et al.  Ultrastructure and chemical composition of lipopolysaccharide extracted from Leptospira interrogans serovar copenhageni. , 1986, Journal of general microbiology.

[5]  B. Adler,et al.  PHAGOCYTOSIS OF VIRULENT AND AVIRULENT LEPTOSPIRES BY GUINEA‐PIG AND HUMAN POLYMORPHONUCLEAR LEUKOCYTES IN VITRO , 1984, Pathology.

[6]  B. Adler,et al.  Immunogenicity of leptospiral vaccines grown in protein-free medium. , 1982, Journal of medical microbiology.

[7]  E. Banfi,et al.  The role of antibodies and serum complement in the interaction between macrophages and leptospires. , 1982, Journal of general microbiology.

[8]  H. Muller,et al.  MATURATION OF HUMORAL IMMUNE RESPONSE DETERMINES THE SUSCEPTIBILITY OF GUINEA‐PIGS TO LEPTOSPIROSIS , 1980, Pathology.

[9]  B. Adler,et al.  The antibodies involved in the human immune response to leptospiral infection. , 1978, Journal of medical microbiology.

[10]  B. Adler,et al.  Serological and protective-antibody responses of rabbits to leptospiral antigens. , 1978, Journal of medical microbiology.

[11]  R. Germain,et al.  Enhancement of carrier-specific helper T cell function by the synthetic adjuvant, N-acetyl muramyl-L-alanyl-D-isoglutamine (MDP). , 1978, Journal of immunology.

[12]  B. Adler,et al.  Host immunological mechanisms in the resistance of mice to leptospiral infections , 1977, Infection and immunity.

[13]  M. Torten,et al.  A new leptospiral vaccine for use in man. I. Development of a vaccine from Leptospira grown on a chemically defined medium. , 1973, The Journal of infectious diseases.

[14]  R. C. Johnson,et al.  Electron Microscopy of Immune Disruption of Leptospires: Action of Complement and Lysozyme , 1968, Journal of bacteriology.

[15]  S. Faine,et al.  VIRULENCE-LINKED COLONIAL AND MORPHOLOGICAL VARIATION IN LEPTOSPIRA , 1964, Journal of bacteriology.

[16]  J. Smith.  Vaccination of guinea-pigs and human beings against leptospiral infections , 1937, Journal of Hygiene.