rhBMP-2 significantly enhances guided bone regeneration-Wikesj-2004-Clinical Oral Implants Research

Background: Previous studies have shown a limited potential for bone augmentation following guided bone regeneration (GBR) in horizontal alveolar defects. Surgical implantation of recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge carrier (ACS) significantly enhances bone regeneration in such defects; however, sufficient quantities of bone for implant dentistry are not routinely obtained. The objective of this studywas to evaluate the potential of rhBMP-2/ACS to enhance GBR using a space-providing,macro-porous expandedpolytetrafluoroethylene (ePTFE) device. Methods: Bilateral, critical size, supra-alveolar, peri-implant defects were surgically created in four Hound Labrador mongrel dogs. Two turned and one surface-etched 10-mm titanium dental implant were placed 5mm into the surgically reduced alveolar ridge creating 5-mm supra-alveolar defects. rhBMP-2/ACS (rhBMP-2 at 0.2mg/ml) or buffer/ACS was randomly assigned to left and right jaw quadrants in subsequent animals. The space-providing, macroporous ePTFE device was placed to cover rhBMP-2/ACS and control constructs and dental implants. Gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analysis. Results: Bone formation was significantly enhanced in defects receiving rhBMP-2/ACS compared to control. Vertical bone gain averaged ( SD) 4.7 0.3 and 4.8 0.1mm, and new bone area 10.3 2.0 and 8.0 2.5mm at turned and surface-etched dental implants, respectively. Corresponding values for the control were 1.8 2.0 and 1.3 1.3mm, and 1.8 1.3and 1.2 0.6 mm. Bone–implant contact in rhBMP-2-induced bone averaged 6.4 1.4% and 9.6 7.5% for turned and surface-etched dental implants, respectively (P1⁄40.399). Corresponding values for the control were 14.6 19.4% and 23.7 9.7% (P1⁄40.473). Bone–implant contact in resident bone ranged between 43% and 58% without significant differences between dental implant surfaces. Conclusions: rhBMP-2/ACS significantly enhances GBR at turned and surface-etched dental implants. The dental implant surface technology does not appear to substantially influence

[1]  R. Nishimura,et al.  Long-term functional loading of dental implants in rhBMP-2 induced bone. A histologic study in the canine ridge augmentation model. , 2003, Clinical oral implants research.

[2]  R. C. Thomson,et al.  Periodontal repair in dogs: space-providing ePTFE devices increase rhBMP-2/ACS-induced bone formation. , 2003, Journal of clinical periodontology.

[3]  R. C. Thomson,et al.  Periodontal repair in dogs: rhBMP-2 significantly enhances bone formation under provisions for guided tissue regeneration. , 2003, Journal of clinical periodontology.

[4]  J. Wozney,et al.  Periodontal repair in dogs: evaluation of a bioabsorbable space-providing macroporous membrane with recombinant human bone morphogenetic protein-2. , 2003, Journal of periodontology.

[5]  J. Wozney,et al.  RhBMP-2/alphaBSM induces significant vertical alveolar ridge augmentation and dental implant osseointegration. , 2002, Clinical implant dentistry and related research.

[6]  J. Wozney,et al.  Bone repair following recombinant human bone morphogenetic protein-2 stimulated periodontal regeneration. , 2002, Journal of periodontology.

[7]  J. Wozney,et al.  Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) on healing in 3-wall intrabony defects in dogs. , 2002, Journal of periodontology.

[8]  T. Sigurdsson,et al.  Alveolar ridge augmentation with rhBMP-2 and bone-to-implant contact in induced bone. , 2001, The International journal of periodontics & restorative dentistry.

[9]  M. Nunn,et al.  Effect of recombinant human bone morphogenetic protein-2 on bone regeneration and osseointegration of dental implants. , 2001, Clinical oral implants research.

[10]  S. Shabahang,et al.  An evaluation of bone induction delivery materials in conjunction with root-form implant placement. , 2001, The International journal of periodontics & restorative dentistry.

[11]  J. Wozney,et al.  Hyaluronan supports recombinant human bone morphogenetic protein-2 induced bone reconstruction of advanced alveolar ridge defects in dogs. A pilot study. , 2001, Journal of periodontology.

[12]  J. Wozney,et al.  Augmentation of Alveolar Bone and Dental Implant Osseointegration: Clinical Implications of Studies with rhBMP-2 A Comprehensive Review , 2001, The Journal of bone and joint surgery. American volume.

[13]  M. Block,et al.  Bone gap healing in the dog using recombinant human bone morphogenetic protein-2. , 2000, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[14]  U. Wikesjö,et al.  Ridge augmentation following implantation of recombinant human bone morphogenetic protein-2 in the dog. , 2000, Journal of periodontology.

[15]  S. Shabahang,et al.  Evaluation of the long-term effect of function on rhBMP-2 regenerated hemimandibulectomy defects. , 1999, The British journal of oral & maxillofacial surgery.

[16]  J. Wozney,et al.  Mandibular Reconstruction Using Bone Morphogenetic ProteinLong‐Term Follow‐up in a Canine Model , 1999, The Laryngoscope.

[17]  R. Schmelzeisen,et al.  Comparison of the late results of mandibular reconstruction using nonvascularized or vascularized grafts and dental implants. , 1999, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[18]  J. Wozney,et al.  Effect of human bone morphogenetic protein 2 implant on tooth eruption in an experimental design. , 1999, The Journal of craniofacial surgery.

[19]  J. Wozney,et al.  Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment. , 1999, Journal of clinical periodontology.

[20]  J. Wozney,et al.  Recombinant human bone morphogenetic protein-2 stimulation of bone formation around endosseous dental implants. , 1999, Journal of periodontology.

[21]  P. Boyne,et al.  Human recombinant BMP-2 in osseous reconstruction of simulated cleft palate defects. , 1998, The British journal of oral & maxillofacial surgery.

[22]  M. Bošković,et al.  Bone formation and reosseointegration in peri-implantitis defects following surgical implantation of rhBMP-2. , 1997, The International journal of oral & maxillofacial implants.

[23]  J. Wozney,et al.  Bone formation and osseointegration stimulated by rhBMP-2 following subantral augmentation procedures in nonhuman primates. , 1997, The International journal of oral & maxillofacial implants.

[24]  D. Cochran,et al.  Radiographic analysis of regenerated bone around endosseous implants in the canine using recombinant human bone morphogenetic protein-2. , 1997, The International journal of oral & maxillofacial implants.

[25]  U. Wikesjö,et al.  Effect of allogeneic, freeze-dried, demineralized bone matrix on guided bone regeneration in supra-alveolar peri-implant defects in dogs. , 1997, The International journal of oral & maxillofacial implants.

[26]  A. Linde,et al.  Importance of delivery systems for growth-stimulatory factors in combination with osteopromotive membranes. An experimental study using rhBMP-2 in rat mandibular defects. , 1997, Journal of biomedical materials research.

[27]  I. Ishikawa,et al.  Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs. , 1997, Journal of periodontology.

[28]  J. Wozney,et al.  Periodontal repair in dogs: evaluation of rhBMP-2 carriers. , 1996, The International journal of periodontics & restorative dentistry.

[29]  J. Wozney,et al.  Maxillary alveolar cleft repair in dogs using recombinant human bone morphogenetic protein-2 and a polymer carrier. , 1996, Plastic and reconstructive surgery.

[30]  P. Boyne.  Animal studies of application of rhBMP-2 in maxillofacial reconstruction. , 1996, Bone.

[31]  C. Kirker-Head,et al.  Bone formation in the goat maxillary sinus induced by absorbable collagen sponge implants impregnated with recombinant human bone morphogenetic protein-2. , 1996, The International journal of periodontics & restorative dentistry.

[32]  D. Buser,et al.  Osseointegration of titanium implants in bone regenerated in membrane-protected defects: a histologic study in the canine mandible , 1996 .

[33]  J. Wozney,et al.  Periodontal repair in dogs: recombinant human bone morphogenetic protein-2 significantly enhances periodontal regeneration. , 1995, Journal of periodontology.

[34]  R. Schenk,et al.  Supracrestal bone formation around dental implants: an experimental dog study. , 1995, The International journal of oral & maxillofacial implants.

[35]  P. Trisi,et al.  Vertical ridge augmentation using a membrane technique associated with osseointegrated implants. , 1994, The International journal of periodontics & restorative dentistry.

[36]  I. Ishikawa.  Regenerative therapy in periodontal diseases. Histological observations after implantation of rhBMP-2 in the surgically created periodontal defects in adult dogs , 1994 .

[37]  D. Buser,et al.  Healing pattern of bone regeneration in membrane-protected defects: a histologic study in the canine mandible. , 1994, The International journal of oral & maxillofacial implants.

[38]  E. Wang,et al.  Mandibular reconstruction with a recombinant bone-inducing factor. Functional, histologic, and biomechanical evaluation. , 1991, Archives of otolaryngology--head & neck surgery.

[39]  V. Rosen,et al.  Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[40]  E. Drier,et al.  OP‐1 cDNA encodes an osteogenic protein in the TGF‐beta family. , 1990, The EMBO journal.

[41]  V. Rosen,et al.  Recombinant human bone morphogenetic protein induces bone formation. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[42]  V. Rosen,et al.  Novel regulators of bone formation: molecular clones and activities. , 1988, Science.

[43]  K. Donath,et al.  A method for the study of undecalcified bones and teeth with attached soft tissues. The Säge-Schliff (sawing and grinding) technique. , 1982, Journal of oral pathology.

[44]  M. Urist,et al.  Bone: Formation by Autoinduction , 1965, Science.