In situ and ultrasensitive DNA detection based on a graphene-coated silica fiber taper interferometer

A high sensitivity biosensor based on graphene coated silica fiber taper interferometer is presented. Thank to the combination of graphene coating and the optical fiber taper interferometer structure, the biosensor demonstrates improved DNA concentration sensitivity of 0.4 nm/log M and good linearity, yielding the lower detection limit of 10 pM. This high sensitivity and biocompatibility enable the biosensor in precision in-situ DNA detection, even in ultra-diluted DNA solution. Based on our work, the graphene coating could convert the concentration information of target molecular to the RI variation, and further to light signals by the taper.

[1]  Bai-Ou Guan,et al.  High-efficiency ultraviolet inscription of Bragg gratings in microfibers , 2012, IEEE Photonics Journal.

[2]  B. Guan,et al.  In-situ DNA hybridization detection with a reflective microfiber grating biosensor. , 2014, Biosensors & bioelectronics.

[3]  Long Jin,et al.  Bending effect on modal interference in a fiber taper and sensitivity enhancement for refractive index measurement. , 2013, Optics express.

[4]  K. Jaruwongrungsee,et al.  Piezoresistive microcantilever-based DNA sensor for sensitive detection of pathogenic Vibrio cholerae O1 in food sample. , 2015, Biosensors & bioelectronics.

[5]  Long Jin,et al.  Temperature-Insensitive Humidity Sensor Based on a Silica Fiber Taper Interferometer , 2013, IEEE Photonics Technology Letters.

[6]  F. Caruso,et al.  Quartz crystal microbalance study of DNA immobilization and hybridization for nucleic Acid sensor development. , 1997, Analytical chemistry.

[7]  Nan Wu,et al.  Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules , 2011, Sensors.

[8]  Xudong Fan,et al.  Optical ring resonators for biochemical and chemical sensing , 2011, Analytical and bioanalytical chemistry.

[9]  Bai-Ou Guan,et al.  Temperature-Compensated Refractive-Index Sensing Using a Single Bragg Grating in an Abrupt Fiber Taper , 2013, IEEE Photonics Journal.

[10]  J Wang,et al.  Mismatch-sensitive hybridization detection by peptide nucleic acids immobilized on a quartz crystal microbalance. , 1997, Analytical chemistry.