Multilocus Sequence Typing System for Group B Streptococcus

ABSTRACT A multilocus sequence typing (MLST) system was developed for group B streptococcus (GBS). The system was used to characterize a collection (n = 152) of globally and ecologically diverse human strains of GBS that included representatives of capsular serotypes Ia, Ib, II, III, V, VI, and VIII. Fragments (459 to 519 bp) of seven housekeeping genes were amplified by PCR for each strain and sequenced. The combination of alleles at the seven loci provided an allelic profile or sequence type (ST) for each strain. A subset of the strains were characterized by restriction digest patterning, and these results were highly congruent with those obtained with MLST. There were 29 STs, but 66% of isolates were assigned to four major STs. ST-1 and ST-19 were significantly associated with asymptomatic carriage, whereas ST-23 included both carried and invasive strains. All 44 isolates of ST-17 were serotype III clones, and this ST appeared to define a homogeneous clone that was strongly associated with neonatal invasive infections. The finding that isolates with different capsular serotypes had the same ST suggests that recombination occurs at the capsular locus. A web site for GBS MLST was set up and can be accessed at http://sagalactiae.mlst.net. The GBS MLST system offers investigators a valuable typing tool that will promote further investigation of the population biology of this organism.

[1]  Stephanie Schrag,et al.  Prevention of Perinatal Group B Streptococcal Disease Revised Guidelines from CDC , 2002 .

[2]  Carmen Buchrieser,et al.  Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease , 2002, Molecular microbiology.

[3]  Ian T. Paulsen,et al.  Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[4]  B. Foxman,et al.  Group B Streptococcus colonization in male and nonpregnant female university students: a cross-sectional prevalence study. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[5]  F. Kong,et al.  Serotype Identification of Group B Streptococci by PCR and Sequencing , 2002, Journal of Clinical Microbiology.

[6]  E. Adderson,et al.  Long-Range Mapping of the Streptococcus agalactiae Phylogenetic Lineage Restriction Digest Pattern Type III-3 Reveals Clustering of Virulence Genes , 2002, Infection and Immunity.

[7]  Martin C. J. Maiden,et al.  Bioinformatics Applications Note Sequence Type Analysis and Recombinational Tests (start) , 2022 .

[8]  L. Hammitt,et al.  Phylogenetic classification of serotype III group B streptococci on the basis of hylB gene analysis and DNA sequences specific to restriction digest pattern type III-3. , 2001, The Journal of infectious diseases.

[9]  Brian G. Spratt,et al.  Multilocus Sequence Typing of Streptococcus pyogenes and the Relationships between emm Type and Clone , 2001, Infection and Immunity.

[10]  A. Schuchat,et al.  Invasive group B streptococcal disease in Maryland nursing home residents. , 2001, The Journal of infectious diseases.

[11]  L. Hammitt,et al.  Genetic Polymorphisms of Group B StreptococcusscpB Alter Functional Activity of a Cell-Associated Peptidase That Inactivates C5a , 2000, Infection and Immunity.

[12]  H. Yim,et al.  The Serotype of Type Ia and III Group B Streptococci Is Determined by the Polymerase Gene within the Polycistronic Capsule Operon , 2000, Journal of bacteriology.

[13]  K. Chow,et al.  Group B streptococcal meningitis in adults: recent increase in Southeast Asia. , 2000, Australian and New Zealand journal of medicine.

[14]  R. Quentin,et al.  Genetic Features of Streptococcus agalactiae Strains Causing Severe Neonatal Infections, as Revealed by Pulsed-Field Gel Electrophoresis and hylB Gene Analysis , 1999, Journal of Clinical Microbiology.

[15]  A. Schuchat.  Group B streptococcus , 1999, The Lancet.

[16]  B. Spratt,et al.  A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. , 1998, Microbiology.

[17]  A. Schuchat.  Epidemiology of Group B Streptococcal Disease in the United States: Shifting Paradigms , 1998, Clinical Microbiology Reviews.

[18]  M. Achtman,et al.  Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[19]  B. Spratt,et al.  Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae , 1998, Molecular microbiology.

[20]  R Staden,et al.  The staden sequence analysis package , 1996, Molecular biotechnology.

[21]  M. Kilian,et al.  Population structure of Streptococcus agalactiae reveals an association between specific evolutionary lineages and putative virulence factors but not disease , 1996, Infection and immunity.

[22]  R. Selander,et al.  Characterization of Streptococcus agalactiae strains by multilocus enzyme genotype and serotype: identification of multiple virulent clone families that cause invasive neonatal disease , 1995, Journal of clinical microbiology.

[23]  H. Etemadi,et al.  Group B Streptococcal Meningitis , 1995 .

[24]  T. Embley.  The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes , 1991, Letters in applied microbiology.

[25]  J. Musser,et al.  Identification of a high-virulence clone of type III Streptococcus agalactiae (group B Streptococcus) causing invasive neonatal disease. , 1989, Proceedings of the National Academy of Sciences of the United States of America.