Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device

Abstract In this study, a natural cotton thread immunoassay device combined with gold nanorod (GNR) reporter probe is developed for the rapid, sensitive and quantitative electrochemical determination of human ferritin, a lung cancer related biomarker. Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device. An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle (GNP) report probe. The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry (ASV) testing, which meet the requirement for clinical diagnosis.

[1]  James F Rusling,et al.  Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer. , 2010, The Analyst.

[2]  Liang Tang,et al.  Gold nanorod biochip functionalization by antibody thiolation. , 2015, Talanta.

[3]  R. Vaia,et al.  Growth Mechanism of Gold Nanorods , 2013 .

[4]  F. Olasagasti,et al.  Miniaturized technology for protein and nucleic acid point-of-care testing , 2012, Translational Research.

[5]  R. G. Freeman,et al.  Preparation and Characterization of Au Colloid Monolayers , 1995 .

[6]  Tien-Sheng Chao,et al.  Label-free and real-time detection of ferritin using a horn-like polycrystalline-silicon nanowire field-effect transistor biosensor , 2016 .

[7]  Niina J. Ronkainen,et al.  Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers , 2014, Materials.

[8]  Tingting Song,et al.  Novel gold nanoparticle trimer reporter probe combined with dry-reagent cotton thread immunoassay device for rapid human ferritin test. , 2015, Analytica chimica acta.

[9]  David Juncker,et al.  Immunochromatographic assay on thread. , 2012, Analytical chemistry.

[10]  G. Whitesides,et al.  Thread as a matrix for biomedical assays. , 2010, ACS applied materials & interfaces.

[11]  B. Limoges,et al.  An electrochemical metalloimmunoassay based on a colloidal gold label. , 2000, Analytical chemistry.

[12]  X. Mao,et al.  Disposable dry-reagent cotton thread-based point-of-care diagnosis devices for protein and nucleic acid test. , 2015, Biosensors & bioelectronics.

[13]  Alaaldin M. Alkilany,et al.  The Many Faces of Gold Nanorods , 2010 .

[14]  N. Morgan,et al.  Electrochemical immunosensors for detection of cancer protein biomarkers. , 2012, ACS nano.

[15]  Ross D. Peterson,et al.  A photonic crystal biosensor assay for ferritin utilizing iron-oxide nanoparticles. , 2014, Biosensors & bioelectronics.

[16]  Wei Shen,et al.  Thread as a versatile material for low-cost microfluidic diagnostics. , 2010, ACS applied materials & interfaces.

[17]  E. S. Jacobs.  Anodic Stripping Voltammetry of Gold and Silver with Carbon Paste Electrodes. , 1963 .

[18]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[19]  Xu Li,et al.  Flow control concepts for thread-based microfluidic devices. , 2011, Biomicrofluidics.