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Dissection of rat hippocampus using high-definition stereoscopic microscope system is demonstrated. Many surgical operation or animal anatomies are anatomized with stereoscopic microscope. With stereoscopic microscope system, the environments of operation is important factor to dissector. Lights, resolution of acquired stereo images, reflected on the liquid, colors and eye fatigue are fatal factors to dissector. We reduced reflections with two incident angle of lights at 45 degree and used a complementary color at the basement and reform the stereoscopic microsystem. Dissector has felt more comfortable after compensation and operation time is more continuous.