Quantitative evaluation of healthy epidermis by means of multiphoton microscopy and fluorescence lifetime imaging microscopy

Background/purpose: Multiphoton microscopy (MPM) enables the assessment of unstained living biological tissue with submicron resolution, whereas fluorescence lifetime imaging microscopy (FLIM) generates image contrast between different states of tissue characterized by various fluorescence decay rates. The aim of this study was to compare the healthy skin of young individuals with that of older subjects, as well as to assess the skin at different body sites, by means of MPM and FLIM.

[1]  P. Elias,et al.  Integrity and permeability barrier function of photoaged human epidermis. , 1997, Archives of dermatology.

[2]  B R Masters,et al.  Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin. , 1997, Biophysical journal.

[3]  B. R. Masters,et al.  Optical Biopsy of In Vivo Human Skin: Multi-photon Excitation Microscopy , 1998, Lasers in Medical Science.

[4]  Andreas Volkmer,et al.  Femtosecond Two‐photon Excited Fluorescence of Melanin* , 1999, Photochemistry and photobiology.

[5]  J. Nürnberger,et al.  Three‐dimensional imaging of human skin and mucosa by two‐photon laser scanning microscopy , 2002, Journal of cutaneous pathology.

[6]  P. Elias,et al.  The aged epidermal permeability barrier: basis for functional abnormalities. , 2002, Clinics in geriatric medicine.

[7]  Iris Riemann,et al.  High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. , 2003, Journal of biomedical optics.

[8]  W. Webb,et al.  Conformational Dependence of Intracellular NADH on Metabolic State Revealed by Associated Fluorescence Anisotropy*♦ , 2005, Journal of Biological Chemistry.

[9]  J. Lévêque,et al.  Skin capacitance imaging, a new technique for investigating the skin surface. , 2006, European journal of dermatology : EJD.

[10]  Chen-Yuan Dong,et al.  Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging. , 2006, Optics letters.

[11]  K. König,et al.  Two-photon microscopes and in vivo multiphoton tomographs--powerful diagnostic tools for tissue engineering and drug delivery. , 2006, Advanced drug delivery reviews.

[12]  Chen-Yuan Dong,et al.  Multiphoton microscopy: a new paradigm in dermatological imaging. , 2007, European journal of dermatology : EJD.

[13]  S. Xie,et al.  Visualizing extracellular matrix and sensing fibroblasts metabolism in human dermis by nonlinear spectral imaging , 2007, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[14]  Hachiro Tagami,et al.  Functional characteristics of the stratum corneum in photoaged skin in comparison with those found in intrinsic aging , 2008, Archives of Dermatological Research.

[15]  N. Ramanujam,et al.  In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia , 2007, Proceedings of the National Academy of Sciences.

[16]  Maria Smedh,et al.  Multiphoton laser scanning microscopy on non-melanoma skin cancer: morphologic features for future non-invasive diagnostics. , 2008, The Journal of investigative dermatology.

[17]  Karsten König,et al.  Clinical multiphoton tomography , 2008, Journal of biophotonics.

[18]  Karsten König,et al.  Morphological skin ageing criteria by multiphoton laser scanning tomography: non‐invasive in vivo scoring of the dermal fibre network , 2008, Experimental dermatology.

[19]  Chen-Yuan Dong,et al.  Multiphoton microscopy in dermatological imaging. , 2009, Journal of dermatological science.

[20]  Karsten König,et al.  Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma. , 2009, The Journal of investigative dermatology.

[21]  Karsten König,et al.  Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis , 2009, Experimental dermatology.

[22]  Maria Smedh,et al.  Multiphoton laser scanning microscopy--a novel diagnostic method for superficial skin cancers. , 2009, Seminars in cutaneous medicine and surgery.