Dispersion of single-walled carbon nanotubes in poly(diallyldimethylammonium chloride) for preparation of a glucose biosensor

Abstract Poly(diallyldimethylammonium chloride) (PDDA) was chosen to disperse single-walled carbon nanotubes (SWCNTs). The optimal conditions to prepare stable PDDA–SWCNTs aqueous dispersions were presented. Then, the positively charged PDDA–SWCNTs composite and negatively charged glucose oxidase (GOD) were employed to fabricate multilayer films on platinum (Pt) electrodes by layer-by-layer self-assembly technique. The consecutive growth of the multilayer films was confirmed by quartz crystal microbalance. Electrochemical measurements were used to study the properties of the proposed biosensor. Results demonstrated that SWCNTs were evenly dispersed within the PDDA films and efficiently improved the conductivity of the resulting films. Among the biosensors, the one based on seven layers of multilayer films got the best performance. It showed wide linear range of 0.05–12 mM, high sensitivity of 63.84 μA/(mM cm 2 ), low detection limit of about 4 μM and small value of the apparent Michaelis–Menten constant, 8.46 mM. In addition, the biosensor also exhibited good suppression of interference and long-term operational stability. This protocol could be used to immobilize other enzymes to construct a range of biosensors.

[1]  Yanxia Hou.  Study of pure urease Langmuir–Blodgett film and application for biosensor development , 2002 .

[2]  S. Iijima.  Helical microtubules of graphitic carbon , 1991, Nature.

[3]  Haoshen Zhou,et al.  Effect of solution pH and ionic strength on the stability of poly(acrylic acid)-encapsulated multiwalled carbon nanotubes aqueous dispersion and its application for NADH sensor. , 2006, Biosensors & bioelectronics.

[4]  Sandro Carrara,et al.  Improved nanocomposite materials for biosensor applications investigated by electrochemical impedance spectroscopy , 2005 .

[5]  Lei Liu,et al.  Tunable single-walled carbon nanotube microstructure in the liquid and solid states using poly(acrylic acid). , 2006, Nano letters.

[6]  L. Mao,et al.  Novel electrochemical method for sensitive determination of homocysteine with carbon nanotube-based electrodes. , 2004, Biosensors & bioelectronics.

[7]  P. He,et al.  Electrochemical DNA biosensors based on platinum nanoparticles combined carbon nanotubes , 2005 .

[8]  H. Fukumoto,et al.  Layer-by-layer self-assembly of polyelectrolyte and water soluble cyanine dye , 1998 .

[9]  M. L. Mena,et al.  Electrochemical biosensors based on colloidal gold–carbon nanotubes composite electrodes , 2007 .

[10]  Minghui Yang,et al.  Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors. , 2006, Biomaterials.

[11]  Beng Kang Tay,et al.  Multilayer assembly of positively charged polyelectrolyte and negatively charged glucose oxidase on a 3D Nafion network for detecting glucose. , 2007, Biosensors & bioelectronics.

[12]  Rodney Andrews,et al.  Carbon nanotube aqueous sol-gel composites: enzyme-friendly platforms for the development of stable biosensors. , 2004, Analytical biochemistry.

[13]  Liang Ding,et al.  Development of an amperometric biosensor based on glucose oxidase immobilized through silica sol–gel film onto Prussian Blue modified electrode , 2004 .

[14]  J. W. Whittaker,et al.  The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor. , 2007, Biosensors & bioelectronics.

[15]  Zhao Song,et al.  Amperometric aqueous sol–gel biosensor for low-potential stable choline detection at multi-wall carbon nanotube modified platinum electrode , 2006 .

[16]  Guodong Liu,et al.  Biosensor based on self-assembling acetylcholinesterase on carbon nanotubes for flow injection/amperometric detection of organophosphate pesticides and nerve agents. , 2006, Analytical chemistry.

[17]  J. Savéant,et al.  Electron Transfer in Organized Assemblies of Biomolecules. Construction and Dynamics of Avidin/Biotin Co-immobilized Glucose Oxidase/Ferrocene Monolayer Carbon Electrodes , 1998 .

[18]  D. Yan,et al.  Polyelectrolyte-functionalized multiwalled carbon nanotubes : preparation, characterization and layer-by-layer self-assembly , 2005 .

[19]  S. Hou,et al.  Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode. , 2007, Biosensors & bioelectronics.

[20]  Yuehe Lin,et al.  Amperometric glucose biosensor based on self-assembling glucose oxidase on carbon nanotubes , 2006 .

[21]  K. Ariga,et al.  Ultrathin films of charged polysaccharides assembled alternately with linear polyions. , 1998, Journal of biomaterials science. Polymer edition.

[22]  S. Shahrokhian,et al.  Application of thionine-nafion supported on multi-walled carbon nanotube for preparation of a modified electrode in simultaneous voltammetric detection of dopamine and ascorbic acid , 2007 .

[23]  L. Qian,et al.  Composite film of carbon nanotubes and chitosan for preparation of amperometric hydrogen peroxide biosensor. , 2006, Talanta.

[24]  Guo-Li Shen,et al.  Amperometric glucose biosensor based on chitosan with improved selectivity and stability , 2004 .

[25]  G. Rivas,et al.  Dispersion of multi-wall carbon nanotubes in polyethylenimine: A new alternative for preparing electrochemical sensors , 2007 .

[26]  Jijun Zhao,et al.  Electronic Properties of Carbon Nanotubes with Covalent Sidewall Functionalization , 2004 .

[27]  P. Pehrsson,et al.  Water-soluble and optically pH-sensitive single-walled carbon nanotubes from surface modification. , 2002, Journal of the American Chemical Society.

[28]  Katsuhiko Ariga,et al.  ASSEMBLY OF MULTICOMPONENT PROTEIN FILMS BY MEANS OF ELECTROSTATIC LAYER-BY-LAYER ADSORPTION , 1995 .

[29]  H. Ju,et al.  Multilayer membranes for glucose biosensing via layer-by-layer assembly of multiwall carbon nanotubes and glucose oxidase. , 2006, Analytical biochemistry.

[30]  J. Xin,et al.  Decoration of carbon nanotubes with chitosan , 2005 .

[31]  G. S. Wilson,et al.  Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer , 1980 .

[32]  Chen Yang,et al.  Study of the nonenzymatic glucose sensor based on highly dispersed Pt nanoparticles supported on carbon nanotubes. , 2007, Talanta.

[33]  Vladimir Tvarozek,et al.  Self-assembled BLMs: biomembrane models and biosensor applications , 1997 .

[34]  R. Yu,et al.  Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode. , 2007, Talanta.