Molecular Cloning of the Gene (SodCc1) that Encodes a Cytosolic Copper/Zinc-Superoxide Dismutase from Rice (Oryza sativa L.)

SOD (superoxide:superoxide oxidoreductase; EC 1.15.1.1) is considered a crucial component in biological defense against oxidative stress (Bowler et al., 1992). In higher plants, there are three known forms of the enzyme: Cu/Zn-, Mn-, and Fe-SODs. These SOD activities are typ- ically associated with specific subcellular locations such as mitochondria (Mn-SOD), plastids (Cu/Zn-SOD and/or Fe- SOD), and the cytosol (Cu/Zn-SOD) and display a differ- ential expression pattern in response to environmental and chemical stresses (Perl-Treves and Galun, 1991; Tsang et al., 1991). We have isolated SOD cDNA clones for a mito- chondrial Mn protein and two cytosolic Cu/Zn isozymes from a rice (Oryza