论文信息 - Analysis of Vitamin B12 in Food by Silica Gel 60 TLC and Bioautography with Vitamin B12-Dependent Escherichia coli 215

Analysis of Vitamin B12 in Food by Silica Gel 60 TLC and Bioautography with Vitamin B12-Dependent Escherichia coli 215

Abstract To evaluate whether certain foods contain vitamin B12 or inactive corrinoids, a simple technique, bioautography with vitamin B12-dependent Escherichia coli mutant after separation of the sample by silica gel 60 thin-layer chromatography, is available. By using the method, vitamin B12-compounds found in some edible cyanobacteria are readily identified. This bioautography has great advantages (simplicity, speed, and inexpensiveness) for the analysis of vitamin B12-compounds in food.

Yuri Tanioka | Y. Yabuta | E. Miyamoto | H. Inui | F. Watanabe

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