Magnetic DNA affinity purification of yeast transcription factor.

Publisher Summary This chapter describes the preparation of magnetic DNA affinity beads and their use in the purification of sequence-specific DNA-binding proteins. The protein chosen for the illustration of the method is yeast transcription factor z––one of the most complex transcription factors characterized so far. The chapter presents an improved variant of the previously reported magnetic DNA affinity purification method: starting with a partially purified protein fraction (purity

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