Synergistic Anti-tumoral Effect of Achillea millefolium Combined with Bleomycin on Prostate Cancer Cells

Background: The aim of this study was to investigate the effec t of methanolic extract of Achillea millefolium L. (MEA) on the antiproliferative activity of bleomycin on human prostate cancer and normal skin cells. Materials and Methods: Human prostate cancer cell (DU-145) and human nonmalignant fibroblast cell (HFFF2) were treated with MEA at various concentrations (20, 100, 500, 1000 and 2000 µg/ml), using bleomycin alone or combined with MEA. Also, the effect of MEA and bleomycin on cell viability was evaluated. Free radical scavenging property was det ermined for this herbal extract. Results: The combination of MEA and bleomycin significantly increased the inhibition of cell growth in cancer cell. The extra ct significantly enhanced cytotoxicity induced by bleomycin showing 60% and 49% survival rate at doses of 1000 and 2000 µg/ml, respectively. In bleomycin-tre ated cells the survival rate reached 85%. MEA did not exhibit any cytotoxicity on HFFF2 cells. Conclusion: This study found that the methanolic extract of Achillea millefolium enhanced the cell toxicity induced by bleomycin in the prostate cancer cell without any significant toxicity on normal cells.

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