ROS Generation and Mitochondrial Dysfunction in the Apoptotic Response to Bortezomib , a Novel Proteasome Inhibitor , in Human H 460 Non-Small Cell Lung Cancer Cells

The abbreviations used are: DYm, mitochondrial membrane potential; DiOC6(3), 3,3’dihexyloacarbocyanine; DTT, 1,4,-dithiothreitol; FACS, fluorescence-activated cell sorter; HEPES, n-(2-hydroxyethyl)piperazine-N’-(4-butanesulfonic acid); MTT, 3-(4,5-dimethylthiazol2yl)-2,5-diphenyltetrazolium bromide; PARP, poly(ADP-ribose) polymerase; PBS, phosphatebuffered saline; ROS, reactive oxygen species; TBST, Tris-buffed saline with Tween 20; Tiron, 4,5-dihydroxy-1,3-benzendisulfonic acid disodium salt; Z-VAD-fmk, benzyloxycarbiny-Val-AlaDL-Aspfluoromethylketone.