Simple sensor for the determination of phenol and its derivatives in water based on enzyme tyrosinase

Abstract Design of a new sensor, based on enzyme tyrosinase, suitable for phenol determination in water was described. The influence of measuring parameters such as pH of the sample and enzyme concentration on analytical performance of the sensor was evaluated. Calibration curves were constructed for catechol, 4-methylcatechol, 4-tertbutylcatechol, phenol, 4-chlorophenol, 2-tertbutylphenol and cresols (p and m isomers of cresol were treated separately) and sensitivity as well as LOD and LOQ were estimated for these phenols. Additionally, the influence of sample matrix components on the electrode response was studied according to the Plackett–Burman experimental design. The following potential interferents which are usually met in waters were taken into account in the examination: Mg 2+ , Ca 2+ , HCO 3 − , SO 4 2− , Cl − as well as Cu 2+ . It was found that among tested ions only Cu 2+ directly affected the electrode response. Determination of phenol index in tap water samples together with recovery studies was also performed. Obtained results suggest that developed sensor can be successfully used for the determination of phenols in concentration range covering its environmental levels.

[1]  Klaus Danzer,et al.  Analytical Chemistry: Theoretical and Metrological Fundamentals , 2006 .

[2]  A. Kende,et al.  Determination of phenols and chlorophenols as trimethylsilyl derivatives using gas chromatography-mass spectrometry. , 2008, Journal of chromatography. A.

[3]  R. Plackett,et al.  THE DESIGN OF OPTIMUM MULTIFACTORIAL EXPERIMENTS , 1946 .

[4]  J. Zabicky.  Analytical Aspects of Phenolic Compounds , 2009 .

[5]  Pradyot Patnaik,et al.  A comprehensive guide to the hazardous properties of chemical substances , 2007 .

[6]  J. L. Hidalgo-Hidalgo-de-Cisneros,et al.  Structural characteristics and cyclic voltammetric behaviour of Sonogel-Carbon tyrosinase biosensors. A detailed comparative study of three immobilization matrixes , 2008 .

[7]  L. Kubota,et al.  Influence of EDTA on the electrochemical behavior of phenols , 2003 .

[8]  G. Wallace,et al.  Development of Conducting Polymer Modified Electrodes for the Detection of Phenol , 2002 .

[9]  A. Calafat,et al.  Automated on-line column-switching HPLC-MS/MS method for measuring environmental phenols and parabens in serum. , 2008, Talanta.

[10]  F. Borrull,et al.  Strategies for determining environmental pollutants at trace levels in aqueous samples by capillary electrophoresis , 1999 .

[11]  P. Spinelli,et al.  Deactivation and regeneration of Pt anodes for the electro-oxidation of phenol , 2005 .

[12]  Cecilia Lete,et al.  Electrochemical sensors based on platinum electrodes modified with hybrid inorganic–organic coatings for determination of 4-nitrophenol and dopamine , 2009 .

[13]  Devendra Kumar,et al.  Recent progress in the development of nano-structured conducting polymers/nanocomposites for sensor applications , 2009 .

[14]  Germano Tremiliosi-Filho,et al.  Electrode passivation caused by polymerization of different phenolic compounds , 2006 .

[15]  L. Fonseca,et al.  Applications of polymers for biomolecule immobilization in electrochemical biosensors , 2008 .

[16]  J. Kochana,et al.  Titania sol–gel-derived tyrosinase-based amperometric biosensor for determination of phenolic compounds in water samples. Examination of interference effects , 2008, Analytical and bioanalytical chemistry.

[17]  M. C. Pietrogrande,et al.  GC-MS analytical methods for the determination of personal-care products in water matrices , 2007 .

[18]  Qingsheng Wu,et al.  Enhancement of surfactants in the electrochemical determination of phenols and the mechanisms research , 2008 .

[19]  F. Hernández,et al.  Determination of priority organic micro-pollutants in water by gas chromatography coupled to triple quadrupole mass spectrometry. , 2007, Analytica chimica acta.

[20]  M. Hasani,et al.  Application of principal component-artificial neural network models for simultaneous determination of phenolic compounds by a kinetic spectrophotometric method. , 2008, Journal of hazardous materials.

[21]  F. E. Suliman,et al.  Analysis of phenols in water by high-performance liquid chromatography using coumarin-6-sulfonyl chloride as a fluorogenic precolumn label. , 2006, Journal of chromatography. A.

[22]  Thorsten Reemtsma,et al.  Liquid chromatography-mass spectrometry and strategies for trace-level analysis of polar organic pollutants. , 2003, Journal of chromatography. A.

[23]  S. Cosnier,et al.  Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix. , 2007, Biosensors & bioelectronics.

[24]  Xiangqin Lin,et al.  Glassy carbon electrodes modified with mixed covalent monolayers of choline, glycine, and glutamic acid for the determination of phenolic compounds , 2006 .

[25]  A. Savall,et al.  Improvement of the electrocatalytic activity of platinum in oxidation of aromatic compounds , 2008 .

[26]  D. Cui,et al.  Adsorption stripping voltammetry of phenol at Nafion-modified glassy carbon electrode in the presence of surfactants. , 2001, Talanta.

[27]  K. Kim,et al.  Gas chromatographic profiling and screening for phenols as isobutoxycarbonyl derivatives in aqueous samples. , 2000, Journal of chromatography. A.

[28]  Baohong Liu,et al.  Phenol biosensor based on Sonogel-Carbon transducer with tyrosinase alumina sol-gel immobilization. , 2008, Analytica chimica acta.

[29]  R. Cela,et al.  Highly selective and efficient determination of US Environmental Protection Agency priority phenols employing solid-phase extraction and non-aqueous capillary electrophoresis. , 2000, Journal of chromatography. A.

[30]  Serge Cosnier,et al.  Calcium carbonate nanoparticles: a host matrix for the construction of highly sensitive amperometric phenol biosensor. , 2007, Biosensors & bioelectronics.

[31]  M. Miró,et al.  Multi-syringe flow injection solid-phase extraction system for on-line simultaneous spectrophotometric determination of nitro-substituted phenol isomers. , 2007, Analytica chimica acta.

[32]  Y. Tsai,et al.  Amperometric biosensors based on multiwalled carbon nanotube-Nafion-tyrosinase nanobiocomposites for the determination of phenolic compounds , 2007 .