Kinetically controlled synthesis of ampicillin and cephalexin in highly condensed systems in the absence of a liquid aqueous phase
暂无分享,去创建一个
C. Ebert | L. Gardossi | A. Basso | P. Linda | M. Toniutti | P. Spizzo
[1] R. Sheldon,et al. Immobilization of Penicillin G Acylase: The Key to Optimum Performance , 2005 .
[2] R. Giordano,et al. Selectivity of the enzymatic synthesis of ampicillin by E. coli PGA in the presence of high concentrations of substrates , 2005 .
[3] Q. Broxterman,et al. Quantitative enzymatic protection of d-amino acid methyl esters by exploiting ‘relaxed’ enantioselectivity of penicillin-G amidase in organic solvent , 2004 .
[4] D. Janssen,et al. Structural and kinetic studies on ligand binding in wild-type and active-site mutants of penicillin acylase. , 2004, Protein engineering, design & selection : PEDS.
[5] V. Svedas,et al. Penicillin acylase‐catalyzed synthesis of β‐lactam antibiotics in highly condensed aqueous systems: Beneficial impact of kinetic substrate supersaturation , 2004, Biotechnology and bioengineering.
[6] D. Janssen,et al. Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of beta-lactam antibiotics. , 2003, European journal of biochemistry.
[7] S. Biffi,et al. Glutaryl-7-ACA acylase catalyses the synthesis of amide bond in heterogeneous substrate mixtures , 2002 .
[8] Vytas K Svedas,et al. Quantitative characterization of the nucleophile reactivity in penicillin acylase-catalyzed acyl transfer reactions. , 2002, Biochimica et biophysica acta.
[9] V. Svedas,et al. Penicillin Acylase‐Catalyzed Solid‐State Ampicillin Synthesis , 2002 .
[10] R. Sheldon,et al. Penicillin acylase-catalyzed ampicillin synthesis using a pH gradient: a new approach to optimization. , 2002, Biotechnology and bioengineering.
[11] D. Janssen,et al. The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase. , 2002, European journal of biochemistry.
[12] C. Ebert,et al. Selectivity of penicillin G acylase towards phenylacetic acid derivatives in amide bond synthesis in toluene , 2001 .
[13] R. Sheldon,et al. Highly efficient synthesis of ampicillin in an "aqueous solution-precipitate" system: repetitive addition of substrates in a semicontinuous process. , 2001, Biotechnology and bioengineering.
[14] C. Ebert,et al. Activity of covalently immobilised PGA in water miscible solvents at controlled aw , 2001 .
[15] C. Ebert,et al. A novel support for enzyme adsorption: properties and applications of aerogels in low water media , 2000 .
[16] R. Sheldon,et al. Penicillin acylase-catalyzed synthesis of ampicillin in "aqueous solution-precipitate" systems. High substrate concentration and supersaturation effect , 2000 .
[17] C. Ebert,et al. d-Phenylglycine and d-4-hydroxyphenylglycine methyl esters via penicillin G acylase catalysed resolution in organic solvents , 2000 .
[18] C. Ebert,et al. Controlling the hydration of covalently immobilised penicillin G amidase in low-water medium : properties and use of Celite R-640 , 2000 .
[19] C. Ebert,et al. High isolated yields in thermodynamically controlled peptide synthesis in toluene catalysed by thermolysin adsorbed on Celite R-640 , 2000 .
[20] R. Sheldon,et al. Enzymatic synthesis of β‐lactam antibiotics using penicillin‐G acylase in frozen media , 1999, FEBS letters.
[21] R. Fernández-Lafuente,et al. The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized penicillin G acylase , 1998 .
[22] C. Ebert,et al. Activity of immobilised penicillin amidase in toluene at controlled water activity , 1998 .
[23] R. Fernández-Lafuente,et al. Use of aqueous two-phase systems for in situ extraction of water soluble antibiotics during their synthesis by enzymes immobilized on porous supports. , 1998, Biotechnology and bioengineering.
[24] C. Ebert,et al. Control of enzyme hydration in penicillin amidase catalysed synthesis of amide bond , 1996 .
[25] E. Bárzana,et al. Effect of pH in the synthesis of ampicillin by penicillin acylase , 1996 .
[26] M. V. Savchenko,et al. Enantioselective Penicillin Acylase—catalyzed Reactions , 1996 .
[27] Min-Gon Kim,et al. Effect of organic solvents on penicillin acylase-catalyzed reactions: interaction of organic solvents with enzymes , 1996 .
[28] Min-Gon Kim,et al. Penicillin acylase-catalyzed synthesis of β-lactam antibiotics in water-methanol mixtures: effect of cosolvent content and chemical nature of substrate on reaction rates and yields , 1996 .
[29] R. Fernández-Lafuente,et al. Immobilization-stabilization of Penicillin G acylase fromEscherichia coli , 1990, Applied biochemistry and biotechnology.
[30] J. Guisán. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes , 1988 .
[31] V. Kasche,et al. Ionic strength and pH effects in the kinetically controlled synthesis of benzylpenicillin by nucleophilic deacylation of free and immobilized phenyl-acetyl-penicillin amidase with 6-aminopenicillanic acid. , 1982, Biochemical and biophysical research communications.
[32] V. Svedas,et al. Enzymatic synthesis of β-lactam antibiotics: A thermodynamic background , 1980 .