Analysis of regional left ventricular function in the post-infarct mouse by magnetic resonance imaging with retrospective gating

Our aim was to test, in a murine myocardial infarction (MMI) model, the feasibility of a new MRI protocol with no ECG/respiratory gating, and compare normal mice versus MMI, in terms of global and regional LV function. A control group (C, 10 mice) and a MMI group (18 mice) were studied. MRI with retrospective gating was performed. End-diastolic (EDV) and end-systolic (ESV) LV volumes with ejection fraction (EF) were computed. In addition, regional fractional area change (RFAC) was used as index of regional wall motion. In MMI, LV size (in muL) was larger compared to C (EDV: 92plusmn25 vs 36plusmn4, ESV: 66plusmn27 vs 10plusmn3), with a significant decrease in EF (31plusmn10 vs 72plusmn7%). RFAC in C showed heterogeneous values. In MMI, RFAC decreased from LV base to apex, with a min (26%) in the anterior segment, in agreement with the occluded LAD. MRI with retrospective gating is feasible in mice, and regional LV function analysis allows the localization of the induced infarction.

[1]  Pamela S Douglas,et al.  Distinct mouse coronary anatomy and myocardial infarction consequent to ligation , 2005, Coronary artery disease.

[2]  Stefan Neubauer,et al.  Quantitative 3-Dimensional Echocardiography for Accurate and Rapid Cardiac Phenotype Characterization in Mice , 2004, Circulation.

[3]  André Constantinesco,et al.  Cardiovascular Disorders: Insights into In Vivo Cardiovascular Phenotyping , 2008 .

[4]  Dara L Kraitchman,et al.  Cardiac magnetic resonance imaging in small rodents using clinical 1.5 T and 3.0 T scanners. , 2007, Methods.

[5]  R G Weiss,et al.  Dynamic magnetic resonance images of murine cardiac function at physiologic heart rates. , 2000, Circulation.

[6]  R M Lang,et al.  Combined Assessment of Myocardial Perfusion and Regional Left Ventricular Function by Analysis of Contrast-Enhanced Power Modulation Images , 2001, Circulation.

[7]  Kieran Clarke,et al.  Fast, high‐resolution in vivo cine magnetic resonance imaging in normal and failing mouse hearts on a vertical 11.7 T system , 2003, Journal of magnetic resonance imaging : JMRI.

[8]  Stuart S Berr,et al.  MR tagging early after myocardial infarction in mice demonstrates contractile dysfunction in adjacent and remote regions , 2002, Magnetic resonance in medicine.

[9]  A Haase,et al.  Developmental changes of cardiac function and mass assessed with MRI in neonatal, juvenile, and adult mice. , 2000, American journal of physiology. Heart and circulatory physiology.

[10]  Qing Nie,et al.  Mouse cardiac surgery: comprehensive techniques for the generation of mouse models of human diseases and their application for genomic studies. , 2004, Physiological genomics.

[11]  Martin Hrabé de Angelis,et al.  Standards of mouse model phenotyping , 2006 .

[12]  N. Richard,et al.  Cardiac and respiratory self‐gated cine MRI in the mouse: Comparison between radial and rectilinear techniques at 7T , 2007, Magnetic resonance in medicine.

[13]  G Allan Johnson,et al.  High-resolution imaging of murine myocardial infarction with delayed-enhancement cine micro-CT. , 2007, American journal of physiology. Heart and circulatory physiology.

[14]  J C Ehrhardt,et al.  Measurement of left ventricular mass in vivo using gated nuclear magnetic resonance imaging. , 1986, Journal of the American College of Cardiology.

[15]  Martin J. Lohse,et al.  Dobutamine-Stress Magnetic Resonance Microimaging in Mice: Acute Changes of Cardiac Geometry and Function in Normal and Failing Murine Hearts , 2001, Circulation research.

[16]  Pierre Croisille,et al.  Cardiac and respiratory double self‐gated cine MRI in the mouse at 7 T , 2006, Magnetic resonance in medicine.

[17]  P. Zardini.  [Left ventricular remodeling after myocardial infarction]. , 1995, Cardiologia.

[18]  W J Manning,et al.  In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation. , 1994, The American journal of physiology.

[19]  S. Cerutti,et al.  Automated interpretation of regional left ventricular wall motion from cardiac magnetic resonance images. , 2006, Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance.

[20]  Kai Hu,et al.  Cardiac magnetic resonance imaging in small animal models of human heart failure , 2003, Medical Image Anal..

[21]  H. Wen,et al.  DENSE: displacement encoding with stimulated echoes in cardiac functional MRI. , 1999, Journal of magnetic resonance.

[22]  Klaas Nicolay,et al.  Comparison between prospective and retrospective triggering for mouse cardiac MRI , 2007, NMR in biomedicine.

[23]  Jun Dazai,et al.  Retrospective gating for mouse cardiac MRI , 2006, Magnetic resonance in medicine.

[24]  Klaas Nicolay,et al.  Quantification of Left Ventricular Volumes and Ejection Fraction in Mice Using PET, Compared with MRI , 2008, Journal of Nuclear Medicine.

[25]  Stuart S Berr,et al.  Simultaneous Evaluation of Infarct Size and Cardiac Function in Intact Mice by Contrast-Enhanced Cardiac Magnetic Resonance Imaging Reveals Contractile Dysfunction in Noninfarcted Regions Early After Myocardial Infarction , 2004, Circulation.

[26]  R. Levy,et al.  Cardiovascular research: decades of progress, a decade of promise. , 1982, Science.

[27]  B. Janssen,et al.  Long‐term structural and functional consequences of cardiac ischaemia–reperfusion injury in vivo in mice , 2004, Experimental physiology.

[28]  Sashwati Roy,et al.  Characterization of the structural and functional changes in the myocardium following focal ischemia-reperfusion injury. , 2008, American journal of physiology. Heart and circulatory physiology.

[29]  V. Ferrari,et al.  Quantitative assessment of regional myocardial function in a rat model of myocardial infarction using tagged MRI , 2004, Magnetic Resonance Materials in Physics, Biology and Medicine.

[30]  Fang Yang,et al.  Myocardial Infarction and Cardiac Remodelling in Mice , 2002, Experimental physiology.

[31]  Stefan Neubauer,et al.  Magnetic resonance microimaging for noninvasive quantification of myocardial function and mass in the mouse , 1998, Magnetic resonance in medicine.

[32]  A. Ross,et al.  PATHOPHYSIOLOGY AND NATURAL HISTORY , 2005 .

[33]  Pierre Croisille,et al.  Gender and Strain Variations in Left Ventricular Cardiac Function and Mass Determined With Magnetic Resonance Imaging at 7 Tesla in Adult Mice , 2007, Investigative radiology.

[34]  C. Higgins,et al.  Interstudy reproducibility of dimensional and functional measurements between cine magnetic resonance studies in the morphologically abnormal left ventricle. , 1990, American heart journal.